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El objetivo de esta monografía es poner a disposición de científicos y estudiantes de países en vías de desarrollo una lectura introductoria en el campo de la química combinatoria y metodologías relacionadas, a través de la descripción del desarrollo de las técnicas más importantes y sus aplicaciones. Si bien la química combinatoria requiere en algunos casos de técnicas y equipamiento específicos, la intención de los autores es demostrar que se trata de una disciplina al alcance de laboratorios con niveles estándares de experiencia y equipamiento.

This book brings together drug design practitioners, all leaders in their field, who are actively advancing the field of quantitative methods to guide drug discovery, from structure-based design to empirical statistical models - from rule-based approaches to toxicology to the fields of bioinformatics and systems biology. The aim of the book is to show how various facets of the drug discovery process can be addressed in a quantitative fashion (ie: numerical analysis to enable robust predictions to be made). Each chapter includes a brief review of the topic showing the historical development of quantitative approaches, a survey/summary of the current state-of-the-art, a selection of well chosen examples with some worked through and an appreciation of what problems remain to be overcome as well as an indication of how the field may develop. After an overview of quantitative approaches to drug design the book describes the development of concepts of'drug-like properties', of quantitative structure-activity relationships and molecular modelling, and in particular, structure-based design approaches to guide lead optimisation. How to manage and describe chemical structures, underpins all quantitative approaches to drug design and these are described in the following chapters. The next chapter covers the value of a quantitative approach, and also the challenge which is to describe the confidence in any prediction, and methods to assess predictive model quality. The later chapters describe the application of quantitative approaches to describing and optimising potency, selectivity, drug metabolism and pharmacokinetic properties and toxicology, and the design of chemical libraries to feed the screening approaches to lead generation that underpin modern drug discovery. Finally the book describes the impact of bioinformatics, current status of predicting ligand affinity direct from the protein structure, and the application of quantitative approaches to predicting environmental risk. The book provides a summary of the current state-of-the-art in quantitative approaches to drug design, and future opportunities, but it also provides inspiration to drug design practitioners to apply careful design, to make best use of the quantitative methods that are available, while continuing to improve them. Drug discovery still relies heavily on random screening and empirical screening cascades to identify leads and drugs and the process has many failures to deliver only a small handful of drugs. With the rapidly escalating costs of drug discovery and development together with spiralling delivery, quantitative approaches hold the promise of shifting the balance of success, to enable drug discovery to maintain its economic viability.

When a biomaterial is placed inside the body, a biological response is triggered almost instantaneously. With devices that need to remain in the body for long periods, such interactions can cause encrustation, plaque formation and aseptic loosening on the surface. These problems contribute to the patient's trauma and increase the risk of death. Electrical properties, such as local electrostatic charge distribution, play a significant role in defining biological interactions, although this is often masked by other factors. This book describes the fundamental principles of this phenomenon before providing a more detailed scientific background. It covers the development of the relevant technologies and their applications in therapeutic devices such as MRSA-resistant fabrics, cardiovascular and urological stents, orthopaedic implants, and grafts. Academic and graduate students interested in producing a selective biological response at the surface of a given biomaterial will find the detailed coverage of interactions at the nanometre scale useful. Practitioners will also benefit from guidance on how to pre-screen many inappropriate designs of biomedical devices long before any expensive, animal or potentially risky clinical trials. Enhanced by the use of case studies, the book is divided in to four topical sections. The final section is dedicated to the application of related topics making the book unique in its pragmatic approach to combining high end interdisciplinary scientific knowledge with commercially viable new technologies. Contributing to the newly emerging discipline of'nanomedicine', the book is written not only by experts from each relevant specialty but also by practitioners such as clinicians and device engineers from industry.

The modern synthetic chemist applies all the tools available to identify the drug-like molecules with the best chances of becoming novel drugs. This book will act as a primer for graduates and postgraduates interested in a career in drug discovery. It covers both synthetic technologies currently impacting medicinal chemistry and emerging areas. The chapters focus on topics including: parallel medicinal chemistry; solid supported reagents; microwave assisted chemistry; flow synthesis, and high throughput reaction screening.

This work presents a definitive interpretation of the current status of and future trends in natural products—a dynamic field at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids, and enzymes. With more than 1,800 color figures, Comprehensive Natural Products II features 100% new material and complements rather than replaces the original work (©1999). - Reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine - Stimulates new ideas among the established natural products research community—which includes chemists, biochemists, biologists, botanists, and pharmacologists - Informs and inspires students and newcomers to the field with accessible content in a range of delivery formats - Includes 100% new content, with more than 6,000 figures (1/3 of these in color) and 40,000 references to the primary literature, for a thorough examination of the field - Highlights new research and innovations concerning living organisms and their distinctive role in our understanding and improvement of human health, genomics, ecology/environment, and more - Adds to the rich body of work that is the first edition, which will be available for the first time in a convenient online format giving researchers complete access to authoritative Natural Products content

Publication of a multi-author textbook on the biomedical applications of synchrotron infrared microspectroscopy was a central element in the workplan of the EU project DASIM (Diagnostic Applications of Synchrotron Infrared Microspectroscopy). The project involved nearly 70 scientists and clinicians from 9 European countries, including all synchrotron facilities that have or are planning an infrared beamline. Together with its international associates from the USA, Canada and Australia, the project brought together essentially all recognized experts in the field. The project aims were to coordinate international research effort and to disseminate the relevant information amongst biological researchers and health care professionals and this multi-author textbook was conceived as the most important measure towards the aim of dissemination. The field of biomedical applications of synchrotron IR microspectroscopy, which has recently seen unprecedented growth, is extremely interdisciplinary, involving synchrotron physicists, spectroscopists, biologists and clinicians, with associated difficulties in getting these experts to understand each other. This multi-author book, from leading world experts, presents all aspects of the field in language that all the disparate experts involved can understand. It demystifies the subject both for clinicians and biologists who find synchrotron physics difficult to understand and for physicists who find medical/biological terminology incomprehensible. The book focuses specifically on biomedical IR spectroscopy using synchrotron light sources with particular emphasis on understandable presentation of necessary background knowledge, digestible summaries of research progress and above all as a practical'how to do it'guide for those working in or wishing to enter the field of biomedical synchrotron IR microspectroscopy and imaging. Key features of the book include:- • a'Fundamentals'section, explaining the basics of synchrotrons and FTIR spectroscopy as well as the needs of clinicians and biologists with respect to these technologies • a'Technical Aspects'section, going into depth on optical issues, sample preparation and study design/data analysis • case studies bringing together these 2 elements through practical examples • Raman microspectroscopy, as an alternative approach, is explored in depth • the foreword is written by Henry Mantsch and Gwynn Williams, the two undisputed experts in the fields of biomedical FTIR spectroscopy and synchrotron IR microspectroscopy respectively

Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

In an ever-increasing domain of activity Amino Acids Peptides and Proteins provides an annual compilation of the world's research effort into this important area of biological chemistry. Volume 31 provides a review of literature published during 1998. Comprising a comprehensive review of significant developments at this biology/chemistry interface each volume opens with an overview of amino acids and their applications. Work on peptides is reviewed over several chapters ranging from current trends in their synthesis and conformational and structural analysis to peptidomimetics and the discovery of peptide-related molecules in nature. The application of advanced techniques in structural elucidation is incorporated into all chapters whilst periodic chapters on metal complexes of amino acids, peptides and beta-lactams extend the scope of coverage. Efficient searching of specialist topics is facilitated by the sub-division of chapters into discrete subject areas allowing annual trends to be monitored. All researchers in the pharmaceutical and allied industries and at the biology/chemistry interface in academia will find this an indispensable reference source.

Bridging the gap between laboratory observations and industrial practices, this work presents detailed information on recombinant micro-organisms and their applications in industry and agriculture. All recombinant microbes, bacteria, yeasts and fungi are covered.

This reference/text covers fundamentals of peptide and protein drug delivery, including such considerations as synthesis, physical chemistry and biochemistry, analysis, proteolytic and transport constraints, pharmacokinetics, and pharmacodynamics; bioavailability from routes of administration, detai

Furnishing the latest interdisciplinary information on the most important and frequently the only investigational system available for discovery programs that address the effects of small molecules on newly discovered enzyme and receptor targets emanating from molecular biology, this timely resource facilitates the transition from classical to high