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This textbook addresses global and local environmental problems and the involvement of microorganisms in their development and remediation. In particular, methodological aspects, some of them molecular genetic, for the study of microbial communities are considered. Overall, the prominent role of microorganisms in various material cycles is presented. In addition to biochemical principles for the degradation of environmental pollutants, the use of microorganisms in environmental biotechnological processes for the purification of air, water or soil as well as in environmentally friendly production processes is discussed. The book is intended for biologists with an interest in environmental microbiological issues, but also for students of process or environmental engineering, geoecology or geology, as well as students of other environmental science disciplines. For the 3rd edition, the authors have completely revised, corrected, updated and supplemented the book.

For centuries, Chinese medicinal materials have been used for therapeutic purposes. Chinese medicinal materials are traditionally identified by their organoleptic characteristics, such as the texture or the odor. With the advancement of DNA technology, a molecular approach has become an important tool to complement organoleptic, morphological, anatomical and chemical techniques for the authentication and quality assurance of Chinese medicinal materials.In 2002, the authors published Authentication of Chinese Medicinal Materials by DNA Technology which is the first international reference in this field. After 20 years, this new edition updates the various useful techniques and describes the new techniques developed for molecular authentication. The procedures of each DNA technique are provided in detail for'step-by-step'experiments.This book is divided into 5 parts with 18 chapters. Part I reviews the current status of molecular authentication and introduces a wide range of DNA techniques. Part II lists the experimental procedures for molecular authentication. Part III describes the DNA fingerprinting-based techniques. Part IV describes the DNA sequencing-based techniques. Finally, Part V provides an account on the recent advancement in molecular authentication, including guidelines for setting up a proper DNA laboratory and concluding remarks.

The field of industrial microbiology involves a thorough knowledge of the microbial physiology behind the processes in the large-scale, profit-oriented production of microbe-related goods which are the subject of the field. In recent times a paradigm shift has occurred, and a molecular understanding of the various processes by which plants, animals and microorganisms are manipulated is now central to industrial microbiology. Thus the various applications of industrial microbiology are covered broadly, with emphasis on the physiological and genomic principles behind these applications. Relevance of the new elements such as bioinformatics, genomics, proteomics, site-directed mutation and metabolic engineering, which have necessitated the paradigm shift in industrial microbiology are discussed.

In his research, David Dannheisig investigates the influence of lysine129 acetylation on the biological function of survivin including alteration of nucleocytoplasmic shuttling as well as dimerization behavior. Since survivin participates in two major hallmarks of oncogenesis, namely cell death inhibition and chromosomal segregation during the cell cycle, it reflects a valuable target in cancer therapy and research. The author establishes proximity-dependent, fluorescence-microscopic methods to quantify the interaction of survivin with the export receptor Crm1 as well as the homodimerization itself. In the future, those systems can be usedto examine the feasible effect of chemical modulators which are targeting these interactions in a cellular background. The outcome achieved is an essential step towards the enhancement of potential cancer therapies.

Genetic engineering is the process of manually adding new DNA to an organism. The goal is to add one or more new traits that are not already found in that organism. Genes are the chemical blueprints that determine an organism's traits. Moving genes from one organism to another transfers those traits. The use of recombinant DNA technology has become commonplace as new products from genetically altered plants, animals, and microbes have become available for human use. Sientists have discovered two new methods capable of enhancing the efficiency of genetic engineering, and these new gene modification techniques could be huge when it comes to future research studies. Whatever may be the techniques used in biotechnology, the ultimate aim is to make use of the ability of cells to produce certain valuable compounds or the genetic improvements of plants and animals for the benefit of mankind. A must have book for students and all those working in biotechnology fields.

Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. - Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish - Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text - Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins - Includes more sample problems in every chapter for readers to practice concepts

A single source reference covering every aspect of biotechnology, Biotechnology Fundamentals, Second Edition breaks down the basic fundamentals of this discipline, and highlights both conventional and modern approaches unique to the industry. In addition to recent advances and updates relevant to the first edition, the revised work also covers ethics in biotechnology and discusses career possibilities in this growing field. The book begins with a basic introduction of biotechnology, moves on to more complex topics, and provides relevant examples along the way. Each chapter begins with a brief summary, is illustrated by simple line diagrams, pictures, and tables, and ends with a question session, an assignment, and field trip information. The author also discusses the connection between plant breeding, cheese making, in vitro fertilization, alcohol fermentation, and biotechnology.Comprised of 15 chapters, this seminal work offers in-depth coverage of topics that include: Genes and Genomics Proteins and Proteomics Recombinant DNA Technology Microbial Biotechnology Agricultural Biotechnology Animal Biotechnology Environmental Biotechnology Medical Biotechnology Nanobiotechnology Product Development in Biotechnology Industrial Biotechnology Ethics in Biotechnology Careers in Biotechnology Laboratory Tutorials Biotechnology Fundamentals, Second Edition provides a complete introduction of biotechnology to students taking biotechnology or life science courses and offers a detailed overview of the fundamentals to anyone in need of comprehensive information on the subject.

Microorganisms play an important role in the maintenance of the ecosystem structure and function. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Much advancement has been taken place in the field of research on bacterial systems. This book summarizes the experimental setups required for applied microbiological studies. Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. Researchers in any field who utilize bacterial systems will find this book very useful. In addition to microbiology and bacteriology, this book will also find useful in molecular biology, genetics, and pathology and the volume should prove to be a valuable laboratory resource in clinical and environmental microbiology, microbial genetics and agricultural research. Unique features • Easy to follow by the users as the experiments have been written in simple language and step-wise manner. • Role of each reagents to be used in each experiment have been described which will help the beginners to understand quickly and design their own experiment. • Each experiment has been equipped with the coloured illustrations for proper understanding of the concept. • Trouble-shootings at the end of each experiment will be helpful in overcoming the problems faced by the users. • Flow-chart of each experiment will quickly guide the users in performing the experiments.

The book is intended for both undergraduates and postgraduates, with a view to encourage them to synthesize what they have learned in traditional courses on their way to making creative contributions in science. The authors claim that scientists already working in related fields will gain an appreciation for the vast sweep of microbial biotechnology, and that the book will be a useful source of information for the nonscientist. The book should indeed perform admirably in these roles, although its use to nonscientists is likely to be limited by the prerequisite specialist knowledge required for an understanding of the processes and approaches described. In the authors'preamble they say that they regard the book to be unique, because they make no artificial attempt to confine their discussion to a single scientific discipline. The book examines the fundamental principles and facts that underline current practical applications of DNA, Fermentors and techniques of Biotechnology, describes these applications and examines future prospects for related technologies. As usual in books on biotechnology, judgments on appropriate categories of products for inclusion have been made. This reviewer considers the content to be appropriate but there are a few surprises. Salient Features • Text Book of Pharmaceutical Biotechnology is a studentfriendly book which aims to simply understand the basic fundamentals of Pharmaceutical Biotechnology. • Perfect merger of fundamentals of all the major areas of Pharmaceutical Biotechnology like Enzymes, Enzyme Immobilization, Immunology, Antigen – Antibody Reaction, Monoclonal Antibodies, Recombinant DNA Technology, Protoplast Fusion, Gene Therapy, Microbial Transformation, Industrial Biotechnology, Biomass and Bioenergy, Animal Cell Culture, Tissue Culture, Healthcare Biotechnology, Biotechnology and Ethics. • Unique examoriented book to help students in honoring their skills for examinations. • The text matter is simple, easy to read and remember. • Additional tables, boxes and charts have been given to enhance the utility value of text. • The outlines give practical based examples. Authors / Publisher2015063001 General/trade13 Epublication based on (print product)15 ISBN-139789380012759Other2015197893800127599789387813076EPUBActive04 ActiveTextbook Of Pharmaceutical BiotechnologyStudium PressStudium PressAuthorAshish Dixit, Pawan Tiwari, Vivekanand Kishan ChatapAFisrtAsecond08 Citizen ofINAgencyUSD - US Dollar109.50000001 For unrestricted sale with exclusive rights in the specific countries or territoriesINROW2015063020150630English382INBiotechnology And Genetics; Pharmacy; The book is intended for both undergraduates and postgraduates, with a view to encourage them to synthesize what they have learned in traditional courses on their way to making creative contributions in science. The authors claim that scientists already working in related fields will gain an appreciation for the vast sweep of microbial biotechnology, and that the book will be a useful source of information for the nonscientist. The book should indeed perform admirably in these roles, although its use to nonscientists is likely to be limited by the prerequisite specialist knowledge required for an understanding of the processes and approaches described. In the authors'preamble they say that they regard the book to be unique, because they make no artificial attempt to confine their discussion to a single scientific discipline. The book examines the fundamental principles and facts that underline current practical applications of DNA, Fermentors and techniques of Biotechnology, describes these applications and examines future prospects for related technologies. As usual in books on biotechnology, judgments on appropriate categories of products for inclusion have been made. This reviewer considers the content to be appropriate but there are a few surprises. Salient Features &

This book provides a comprehensive description of phosphate solubilizing microorganisms and highlights methods for the use of microphos in different crop production systems. The focus is on understanding both the basic and applied aspects of phosphate solubilizing microorganisms and how phosphorus-deficient soils can be transformed into phosphorus-rich ones by applying phosphate solubilizing microorganisms. The interaction of rhizosphere phosphate solubilizing microorganisms and environmental variables, as well as their importance in the production of crops such as legumes, cereals, vegetables etc. are discussed and considered. The use of cold-tolerant phosphate solubilizing microorganisms to enhance crop productivity in mountainous regions is examined, as are the ecological diversity and biotechnological implications of phosphate solubilizing microorganisms. Lastly, the role of phosphate solubilizing microorganisms in aerobic rice cultivation is highlighted. This volume offers a broad overview of plant disease management using phosphate solubilizing microbes and presents strategies for the management of cultivated crops. It will therefore be of special interest to both academics and professionals working in the fields of microbiology, soil microbiology, biotechnology and agronomy, as well as the plant protection sciences. This timely reference book provides an essential and comprehensive source of material, as it includes recent findings on phosphate solubilizing microorganisms and their role in crop production.

Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enablesthe reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology

Preface Adhesion is a phenomenon architects and civil engineers are not very familiar with. In other disciplines knowledge about surface properties and the background of bonding energies is also far from satisfactory; nevertheless there are many important· applications in concrete engineering, where adhesion is necessary for success and durability. These include: - coating and painting - repair of concrete surfaces - bonding of fresh to old concrete - crack injection - glueing of precast elements - glueing of steel to concrete, etc. In 1981 RILEM established the technical committee 52-RAC'Resin Adherence to Concrete'. The main aims of the committee's work were - to collect research results and practical experiences - to initiate and coordinate research programs - to develop, on a scientific base, test methods for field and for laboratory purposes. One of the results of the committee's work is a state-of-the-art report, which will be presented orally as a General Report at the International Symposium ISAP'86, and will be printed either in the RILEM journal Materials and Structures or separately. Several test recommendations have been elaborated and will be prepared as drafts for the participants ofISAP'86. These are: - direct tensile test - pull-off test - direct shear test - slant shear test - four-point bending test - dynamic loading test - thermal compatibility test (two versions) - injectibility test.

This book imparts an enriching experience to the readers by providing extensive information about the techniques in biotechnology. The fundamentals in this book are critical to the success of researchers and students alike. This book covers techniques of DNA isolation, nucleic acid hybridization, RNA isolation, PCR, molecular marker techniques, Immune techniques, spectroscopy and other techniques applied in biotechnology. The book not only provides the protocols for various techniques but also gives theoretical information regarding it. The principle and working of each technique clarifies the concepts and enhances understanding of the subject. Step-by-step protocols are provided along with preparations of solutions and buffers. Numerous illustrations and diagrammatic representations of the protocol add pictorial value to the theoretical explanation. Troubleshooting guide is an invaluable tool and unique feature of this book, which will help researchers in avoiding pitfalls and obtaining targeted results. Review questions at the end of each chapter, is one more reader friendly unique feature, which will be useful for the teachers, students and researchers alike. The topics covered in this book are in accordance with the postgraduate syllabi of UGC recognized universities and institutions. This book will also be helpful for those who are preparing for competitive exams like NET, JRF, GATE, SET, SRF etc., and for the ones who are engaged in biotechnology research.

Real time quantitative PCR (qPCR) technology has revolutionized almost all areas of microbiology including clinical microbiology, food microbiology, industrial microbiology, environmental microbiology and microbial biotechnology. Various modifications and improvements have enhanced the overall performance of this highly versatile technology and the qPCR instrumentation and strategies currently available are more sensitive, faster and affordable than ever before. Written by experts in the field and aimed specifically at microbiologists, this volume describes and explains the most important aspects of current qPCR strategies, instrumentation and software. Renowned authors cover the application of qPCR technology in various areas of applied microbiology and comment on future trends. Topics covered include instrumentation, fluorescent chemistries, quantification strategies, data analysis software, environmental microbiology, water microbiology, food microbiology, gene expression studies, validation of microbial microarray data and future trends in qPCR technology. The editor and authors have produced an outstanding book that will be invaluable for all microbiologists. A recommended book for all microbiology laboratories.

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts

The book is of interest to academic and industrial researchers but also clinicians who are working on inflammation related topics and would like to extend their knowledge about microarrays and the application thereof. After reading the book a researcher or clinician should be in the position to plan, perform and analyse or to critically review microarray experiments related to inflammation research. So far, non of the published books covers all aspects which need to be taken into account when planning and performing a microrray experiment in inflammation research. Normally, for a researcher or clinician doing a microarray experiment it is not enough to have knowledge about microarrays, as the experiment does not start with purified and quality controlled RNA and does not end with signal intensities. Things like appropriate cell sorting techniques, stabilisation and transport of RNA or cells, the knowledge how to deal with whole blood as well as options for automation are crucial for a successful application of microarrays. Moreover these aspects are the main obstacles for transferring microarray based inflammation analysis from bench to bedside and the limiting factors for higher through put as needed in multicenter studies.

An emerging, ever-evolving branch of science, bioinformatics has paved the way for the explosive growth in the distribution of biological information to a variety of biological databases, including the National Center for Biotechnology Information. For growth to continue in this field, biologists must obtain basic computer skills while computer spe

Description based on print version record.

Calculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. Mathematics, as a vital component of the successful design and interpretation of basic research, is used daily in laboratory work. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis.•A comprehensive guide to calculations for a wide variety of problems encountered in the basic research laboratory.• Example calculations are worked through from start to finish in easy-to-follow steps• Key chapters devoted to calculations encountered when working with bacteria, phage, PCR, radioisotopes, recombinant DNA, centrifugation, oligonucleotides, protein, and forensic science.•Written for students and laboratory technicians but a useful reference for the more experienced researcher.•A valuable teaching resource.

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.Key Features• Includes a glossary of over 200 terms important for understanding molecular biology• Uses an inexpensive source of eukaryotic cells - great for schools on a budget• Includes Methods Locator that provides instant access to the latest methods• Contain clearly written, easy-to-follow, student-tested instructions:• Sterile techniques• Phage titration• Gel electrophoresis of DNA• Restriction enzyme digestion• Plasmid isolation• Transformation of E. Coli• Recombinant DNA cloning• Nick translation labeling • Nonradioactive primer labelling• Nonradioactive DNA detection• Southern blotting• Colony hybridization• Purification of plant DNA• RNA purification• Northern blotting• Purification of poly A+ RNA• Polymerase chain reaction (PCR)

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! - Concepts explained - Methods detailed - Trouble-shooting emphasized - Novel applications highlighted - Key concepts for PCR - Analysis of PCR products - Research applications - Alternative amplification strategies