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Plant Stress Tolerance: Molecular Mechanisms and Breeding Strategies, Volume One provides effective ways for organizing precision and sustainable agriculture. The methods include the use of advanced molecular techniques covering multiple omics, high-throughput technology, computational biology, epigenetic manipulation, and CRISPR genome editing. These methods can advance the development of high-yield, high-quality, and stress-resilient crops that meet the requirements for supporting global food and nutrition security.The book proposes strategies for omics-assisted and speed breeding techniques, exploring molecular mechanisms of plant abiotic stress caused by temperature, drought, salinity, and various pollutants. These are uncovered by quantitative trait loci analysis and mapping, genomic selection, functional genomics, multiple omics, high-throughput sequencing, and high-throughput phenotyping, and are integrated into the various systems of crop improvement.Plant Stress Tolerance: Molecular Mechanisms and Breeding Strategies, Volume One presents emerging and comprehensive knowledge and is an ideal reference for students, researchers, teachers, and professors. It inspires ideas for investigations in the fields of plant stress physiology, plant functional genomics, plant multiple omics, plant genetic engineering, systems biology, and crop breeding.

This is a great book to explore the science underlying the Food Biotechnology, which explores and presents current biotechnological advances and approaches to improving the nutritional value of modern-foods. Novel fermentation and enzyme technological processes, protein engineering, genetic engineering, metabolic engineering, bioengineering, quorum sensing and nanobiotechnology have been incorporated to fetch into new dimensions in current food biotechnology research and this book provides a deep insight on all these aspects as a comprehensive resource for anybody interested in all the types of foods, latest processing, preservation technology and safety. Written by leading scientists in the field, the book will be a valuable resource for students and researchers in the fields of food chemistry, nutritional science, taste physiology, and neuroscience, as well as for professionals in the food industry.

This edited book deals with the distribution, classification and diversity of halophytic ecosystems, ecology of mangroves, coastal agroforestry, adaptations and mechanisms of salt tolerance in glycophytes verses halophytes, scope of biosaline agriculture, and potential utilization of halophytes in abiotic stressed environments in arid and semiarid regions and coastal areas. In this era of global population increase and global environmental change, there is need to provide food to the ever-growing population, combating climate change and conserving biodiversity. Keeping in view the rich biodiversity of halophytes, there is wide scope in food industry, phytoremediation, as well as a source of bioactive compounds including modern drugs. The new technologies for the cultivation of halophytes help to utilize saline and arid wastelands and also waterlogged areas sustainably for humans and the livestock. This book creates interest in educationists, researchers, industrialists, investors, soil and climate change scientists, development/extension workers, environmentalists, policy/decision makers, and government and non-government organizations. Also, the book serves as reference material for undergraduate and graduate students of agriculture, ecology, soil science, and environmental sciences. National and international soil and agricultural scientists, ecologists, policy makers will also find this book immensely useful.

This compendium presents comprehensive information on more than 25 important spice crops commercially grown in India and traded globally, apart from over 40 spices that have the potential to be popularized. In 70 chapters the book covers the achievements in research and development made in India for the past 75 years in various organizations including research institutes, agricultural universities and private sector laboratories. Spices are natural products of plant origin, used primarily for flavouring and seasoning or for adding pungency and flavour to foods and beverages. The flavour and fragrance of Indian spices had a magic spell on human culture since very ancient days. The importance of spices in Indian life and its contribution to the economy are substantial. India, as the world's leading producer of spices is also a significant stakeholder in spices export trade globally. Indian spices being sources of many high value compounds, are also gaining muchimportance for other diversified uses especially for their pharmaceutical and nutraceutical properties. A wide variety of 52 spices are grown in India including black pepper, chillies, cardamom, ginger, turmeric, cinnamon, nutmeg, garlic, onion, cumin, coriander, saffron and vanilla. This book complies a comprehensive, holistic review on the subject, written by the best experts in the field in India representing diverse agencies. This book is a single point reference book for all those involved in the research, study, teaching and use of spices in India and abroad.

This textbook provides an introduction to the fundamental and applied aspects of biophysics for advanced undergraduate and graduate students of physics, chemistry, and biology. The application of physics principles and techniques in exploring biological systems has long been a tradition in scientific research. Biological systems hold naturally inbuilt physical principles and processes which are popularly explored. Systematic discoveries help us understand the structures and functions of individual biomolecules, biomolecular systems, cells, organelles, tissues, and even the physiological systems of animals and plants. Utilizing a physics- based scientific understanding of biological systems to explore disease is at the forefront of applied scientific research.This textbook covers key breakthroughs in biophysics whilst looking ahead to future horizons and directions of research. It contains models based on both classical and quantum mechanical treatments of biological systems. It explores diseases related to physical alterations in biomolecular structures and organizations alongside drug discovery strategies. It also discusses the cutting- edge applications of nanotechnologies in manipulating nanoprocesses in biological systems.Key Features:• Presents an accessible introduction to how physics principles and techniques can be used tounderstand biological and biochemical systems.• Addresses natural processes, mutations, and their purposeful manipulation.• Lays the groundwork for vitally important natural scientific, technological, and medical advances.

This book uncovers the basic principles of microbial photosynthesis and the latest technological interventions of this crucial phenomenon. In the recent past, the basic principles of microbial photosynthesis were technologically articulated to engineer several cell factories that can utilize waste resources and generate different groups of industrially valuable products. Also, the list of model organisms for specific usage have been increasing enormously. This volume covers the material in four sections; each of the part dealing with the basic principles of microbial photosynthesis in an applied orientation focusing on waste valorization and circular bioeconomy. Furthermore, the following chapters deal with the very recent advancements in metabolic engineering and artificial photosynthesis with respect to value addition. Not only will this book be available for graduate and postgraduate students in microbiology, biotechnology, plant sciences, environmental sciences, energy engineering, and renewable energy, it is also an excellent material for researchers needing a multidisciplinary approach.

This book offers a comprehensive analysis of microalgal cultivation methods and optimization of astaxanthin production for various applications, including clinical uses, algae polymers, proteins and pigments, food applications and packaging, algae forming, cosmetics, and more. Microalgae are unicellular living forms and are the primary producers that play a major role in the ecosystem. Commercially, while many documents are available, some recent fields are yet to be explored. The book comprises 19 chapters contributed by experts and reviews the recent developments in the cultivation, harvest, and genetic engineering of H. pluvialis-derived astaxanthin. It also discusses their bottlenecks and challenges in commercial-scale production, as well as current and prospective global market. Current research supports the exploration of new topics and practical applications of microalgae and their products, which will also benefit academia. The book will be an importantresource for researchers and industry, providing comprehensive knowledge on broad topics. Flow charts, updated methods, and colour images are included to help the readers'understanding.

The crop plants cater not only to our basic F5 (food, feed, fiber, fuel, and furniture) needs but also provide a number of nutraceuticals with potential nutritional, safety and therapeutic properties. Many crop plants provide an array of minerals, vitamins, and antioxidant-rich bioactive phytochemicals. Increasing incidences of chronic diseases such as cancer, diabetes and HIV, and malnutrition necessitate global attention to health and nutrition security with equal emphasis to food security. This compendium compiles results of researches on biochemical, physiological and genetic mechanisms underlying biosynthesis of the health and nutrition related nutraceuticals. It also explores the precise breeding strategies for augmentation of their content and amelioration of their quality in crop plants under all commodity categories including cereals and millets, oilseeds, pulses, fruits and nuts, and vegetables. The compendium comprise 5 sections dedicated to these 5 commodity groups and presents enumeration on the concepts, strategies, tools and techniques of nutraceutomics. These sections include 50 chapters devoted to even number of major crop plants. These chapters present deliberations on the biochemistry and medicinal properties of the nutracuticals contained; genetic variation in their contents; classical genetics and breeding for their quantitative and qualitative improvement; tissue culture and genetic engineering for augmentation of productivity and quality; and sources of genes underlying their biosynthesis. They also include comprehensive enumeration on genetic mapping of the genes and QTLs controlling the contents and profile of the nutraceuticals and molecular breeding for their further improvement through marker assisted selection and backcross breeding tools. Prospects of post-genomic precise breeding strategies including genome-wide association mapping, genomic selection, allele mining, and genome editing are also discussed. This compendium fills the gap in academia, and research and development wings of the private sector industries interested in an array of subjects including genetics, genomics, tissue culture, genetic engineering, molecular breeding, genomics-assisted breeding, bioinformatics, biochemistry, physiology, pathology, entomology, pharmacognosy, IPR, etc., and will also facilitate understanding of the policy making agencies and people in the socio-economic domain and research sponsoring agencies.

This book provides a comprehensive introduction and review of state-of-the-art biotechnological tools in enhancement of bioactive compounds in medicinal and aromatic plants. Plant bioactive compounds are plant-based natural products that display a variety of pharmacological applications. These bioactive compounds are important as medicines, pigments, flavorings and drugs because most of the pharmaceutical industries are highly dependent on medicinal plants and their extraction. The book introduces a systematic overview of state-of-the-art biotechnological techniques such as Omics, Crisper /Cas9 and RNAi to enhance the plant bioactive contents as well as enlists various in vitro techniques, hairy root culture and transgenic technology to enhance plant bioactive contents using plant tissue culture approaches. The book also provides an overview of the role of induced mutation, biotic and abiotic stress to increase the bioactive contents in plants and discusses the significant role of endophytes to enhance the contents of plant bioactive compound. The book also opens discussions related to standard operating procedures using hydroponics system of cultivation for significant enhancement of bioactive compound(s) and much more. This book serves as an excellent reference book for the researchers working in the field of plant secondary metabolites and pharmaceutical industries at global level.

Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms.Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement.Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Diatoms are the most species rich group of algae, and they contribute about 20% of annual global carbon fixation. They play major roles in ocean food webs and global biogeochemical cycles. They are also a target of the biotechnology industry because of their nano-patterned silica cell wall and high lipid content. Diatoms have received increasing attention as more genomes became available and because of the development of genome editing tools such as the CRISPR/Cas9 technology, which has made diatoms as genetically tractable as well-established biological model species. This book provides an overview on diatom molecular biology. It brings together international leading experts in the field to discuss the latest data and developments from genes to ecosystems. As the understanding of diatoms is currently experiencing a step change, it is critical to allow for synergistic approaches on diverse aspects of diatom biology and evolution. The books offers fundamental insights into the molecular life of diatoms; at the same time new scientific concepts are developed based on the application of the latest molecular tools and genomic information to explore the fascinating lifestyle of diatoms.

Cotton, the most important natural fiber crop, has been improved by conventional breeding—largely through planned hybridization of different cotton genotypes, since the discovery of Mendelian genetics. All these efforts resulted in the development of resilient high yielding cotton varieties. However, the progress through conventional breeding procedures is slow because of long lag periods for developing a variety, little control over the new genetic combinations, unwanted traits and lack of foolproof performance testing system. Genomic assays discovered over the last two decades have made it possible to understand the “language” of the genome by associating the genes with specific traits. Together with the more recently established gene-editing tools like CRISPR-Cas9, the cotton genome can be tailored much more precisely than ever before. In this regard, genetic information has been harnessed, through (i) sequencing of the progenitor and cultivated cotton species, (ii) ongoing mega pan-genome sequencing projects, (iii) genetic and physical mapping, and (iv) introgression of genes from alien sources, that resulted in the development of resilient cotton cultivars. These technologies have been deployed or are attempting to overcome the challenges of water shortage, excessive heat in most cotton growing regions, infectious diseases and infestation of insect pests, as well as rising production cost, for sustainable cotton production beyond 2030. In this book, new knowledge generated by the cotton research community and its application for developing resilient cotton are comprehensively summarized. This book contributed by well-known cotton researchers is a timely collection of the challenges and successes of precision cotton breeding in a changing environment.

Agriculture faces many challenges to fulfil the growing demand for sustainable food production and ensure high-quality nutrition for a rapidly growing population. To guarantee adequate food production, it is necessary to increase the yield per area of arable land. A method for achieving this goal has been the application of growth regulators to modulate plant growth. Plant growth regulators (PGRs) are substances in specific formulations which, when applied to plants or seeds, have the capacity to promote, inhibit, or modify physiological traits, development and/or stress responses. They maintain proper balance between source and sink for enhancing crop yield. PGRs are used to maximize productivity and quality, improve consistency in production, and overcome genetic and abiotic limitations to plant productivity. Suitable PGRs include hormones such as cytokinins and auxins, and hormone-like compounds such as mepiquat chloride and paclobutrazol. The use of PGRs in mainstream agriculture has steadily increased within the last 20 years as their benefits have become better understood by growers. Unfortunately, the growth of the PGR market may be constrained by a lack of innovation at a time when an increase in demand for new products will require steady innovation and discovery of novel, cost-competitive, specific, and effective PGRs.A plant bio-stimulant is any substance or microorganism applied to plants with the aim to enhance nutrition efficiency, abiotic stress tolerance and/or crop quality traits, regardless of its nutrients content. Apart from traditional PGRs, which are mostly plant hormones, there are a number of substances/molecules such as nitric oxide, methyl jasmonate, brassinosteroids, seaweed extracts, strigolactones, plant growth promoting rhizobacteria etc. which act as PGRs. These novel PGRs or bio-stimulants have been reported to play important roles in stress responses and adaptation. They can protect plants against various stresses, including water deficit, chilling and high temperatures, salinity and flooding. This book includes chapters ranging from sensing and signalling in plants to translational research. In addition, the cross-talk operative in plants in response to varied signals of biotic and abiotic nature is also presented. Ultimately the objective of this book is to present the current scenario and the future plan of action for the management of stresses through traditional as well as novel PGRs. We believe that this book will initiate and introduce readers to state-of-the-art developments and trends in this field of study.

This book assembles state-of-the-art approaches for harnessing light energy as a model to develop natural systems such as biofuels. After the basics and potential of photosynthesis of microalgae it discusses topics from engineering micro-algae towards increased photosynthetic efficiency till the optimization of photobioreactor techniques for enhanced biotechnological applications such as cyanobacteria.

This book encompasses current knowledge of soil microbiomes and their potential biotechnological application for plant growth, crop yield, and soil health under the natural as well as harsh environmental conditions for sustainable agriculture. The microbes are ubiquitous in nature. The soil is a natural hotspot of the soil microbiome. The soil microbiome plays a critical role in the maintenance of global nutrient balance and ecosystem functioning. The soil microbiomes are associated with plant ecosystems through the intense network of plant–microbe interactions. The microbes present in bulk soil move toward the rhizospheric region due to the release of different nutrients by plant systems. The rhizospheric microbes may survive or proliferate in rhizospheric zone depending on the extent of influences of the chemicals secreted into the soil by roots. The root exudates contain the principal nutrients factors (amino acids, glucose, fructose, and sucrose). The microbes present in rhizospheric region have capabilities to fix atmospheric nitrogen, produce different phytohormones, and solubilize phosphorus, potassium, and zinc. The plant systems take these nutrients for their growth and developments. These soil and plant associated microbes also play an important role in protection of plants from different plant pathogenic organisms by producing different secondary metabolites such as ammonia, hydrogen cyanide, siderophores, and hydrolytic enzymes. The soil microbiomes with plant growth-promoting (PGP) attributes have emerged as an important and promising tool for sustainable agriculture. The soil microbiomes promote the plant growth and enhance the crop yield and soil fertility via directly or indirectly different plant growth-promoting mechanism. The soil microbes help the plant for adaptation in extreme habitats by mitigating the abiotic stress of high/low temperatures, hypersalinity, drought, and acidic/alkaline soil. These PGP microbes are used as biofertilizers/bioinoculants to replace the harmful chemical fertilizers for sustainable agriculture and environments.The aim of the book “Soil Microbiomes for Sustainable Agriculture” is to provide the recent advances in mechanisms of plant growth promotion and applications of soil microbiomes for mitigation of different abiotic stresses in plants. The book is useful to scientists, researchers, and students related to microbiology, biotechnology, agriculture, molecular biology, environmental biology, and related subjects.

This book provides all aspects of the physiology, stress responses and tolerance to abiotic stresses of the Brassicaceae plants. Different plant families have been providing food, fodder, fuel, medicine and other basic needs for the human and animal since the ancient time. Among the plant families, Brassicaceae has special importance for their agri-horticultural importance and multifarious uses apart from the basic needs. Interest understanding the response of Brassicaceae plants toward abiotic stresses is growing considering the economic importance and the special adaptive mechanisms. The knowledge needs to be translated into improved elite lines that can contribute to achieve food security. The physiological and molecular mechanisms acting on Brassicaceae introduced in this book are useful to students and researchers working on biology, physiology, environmental interactions and biotechnology of Brassicaceae plants.

'Microalgae Biotechnology for Food, Health and High Value Products” presents the latest technological innovations in microalgae production, market status of algal biomass-based products, and future prospects for microalgal applications. It provides stimulating overviews from different perspectives of application that demonstrate how rapidly the commercial production of microalgae-based food, health and high value products is advancing. It also addresses a range of open questions and challenges in this field. The book highlights the latest advances of interest to those already working in the field, while providing a comprehensive overview for those readers just beginning to learn about the promise of microalgae as a sustainable source of both specialty and commercial products. It offers a valuable asset for commercial algae producers, algae product developers, scientific researchers and students who are dedicated to the advancement of microalgae biotechnology forapplications in health, diet, nutrition, cosmetics, biomaterials etc.

This book focuses on the conventional breeding approach, and on the latest high-throughput genomics tools and genetic engineering / biotechnological interventions used to improve rice quality. It is the first book to exclusively focus on rice as a major food crop and the application of genomics and genetic engineering approaches to achieve enhanced rice quality in terms of tolerance to various abiotic stresses, resistance to biotic stresses, herbicide resistance, nutritional value, photosynthetic performance, nitrogen use efficiency, and grain yield. The range of topics is quite broad and exhaustive, making the book an essential reference guide for researchers and scientists around the globe who are working in the field of rice genomics and biotechnology. In addition, it provides a road map for rice quality improvement that plant breeders and agriculturists can actively consult to achieve better crop production.

New research tools have revealed many surprising aspects of the dynamic nature of lipids and their participation in processes such as recognition, intra- and inter-cellular signalling, deterrence and defense against pathogens, membrane trafficking and protein function. This is in addition to new information on the more established roles of plant lipids as structural components of membranes and as long-term storage products. Plant lipids are also increasingly being seen as sources of a new generation of environmentally friendly, biodegradable, and renewable industrial products, including biopolymers and high-grade lubricants.Plant Lipids: Biology, Utilisation and Manipulation provides a broad overview of plant lipid research and its many applications. Linking various disciplines, the editor brings together researchers from major international laboratories to review the history and current state of progress in this quickly evolving field. The text starts by providing a fascinating historical perspective on the study of plant lipids, from its inception as a branch of alchemy in the seventeenth century to the current post-genomic era. It then offers a detailed discussion on the formation, modification and utilization of fatty acids. This is followed by an exploration of the major classes of macromolecular structures formed by plant lipids, including bilayer membranes and storage bodies. From there, the contributors consider other types of macromolecular lipid assemblies in plants, examining proteins and the key plant lipid structure - the cuticle. The final chapters look at diverse classes of plant lipids that are linked to various aspects of signaling. This text provides an excellent resource for researchers and professionals in plant biochemistry, molecular biology, biotechnology and genetics, in both the academic and industrial sectors. It also meets the needs of students looking for a comprehensive introduction to this field, as well as direction for fut

The use of fossil fuels results in rising CO2 and other greenhouse gas (GHG) emissions, causing global temperature rise and climate change that will negatively impact human health, the food supply, and eventually worsen hunger and misery. Presently, fossil fuels meet 88% of the energy demand, resulting in rising CO2/GHG emissions at alarming rates. The increased use of biofuels would help to mitigate climate change. Efficiently designing methods for the production of biofuels and plant-derived high-value products requires a deeper understanding of photosynthetic processes as a prerequisite for applying novel biotechnologies. Accordingly, this book provides ample information and a wealth of illustrative examples. The book's eighteen richly illustrated chapters are divided into three thematic parts. I: Photosynthesis and Biomass Production under Changing Conditions, II: Microalgae and Engineered Crops for Production of Biofuels and High-value Products, and III: Genetic Resources and Engineering Methods to Improve Crop Plants. Readers will find the latest information on the molecular basis of photosynthetic processes in plants (including the regulatory principles that allow plants to maintain homeostasis under changing conditions), stress resistance and synthetic pathways. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops capable of growing under stress conditions with improved productivity, are presented. The book sets the course for future research in the field of biofuel development and production and provides both general and specific information for students, teachers, academic researchers, industrial teams, and general readers who are interested in new developments concerning the production of biofuels with value-added properties.

Industrial Biotechnology summarizes different aspects of plant biotechnology such as using plants as sustainable resources, phytomedical applications, phytoremedation and genetic engineering of plant systems. These topics are discussed from an academic as well industrial perspective and thus highlight recent developments but also practical aspects of modern biotechnology.

This book provides an up-to-date review and analysis of the carrot's nuclear and organellar genome structure and evolution. In addition, it highlights applications of carrot genomic information to elucidate the carrot's natural and agricultural history, reproductive biology, and the genetic basis of traits important in agriculture and human health. The carrot genome was sequenced in 2016, and its relatively small diploid genome, combined with the fact that it is the most complete root crop genome released to date and the first-ever Euasterid II genome to be sequenced, mean the carrot has an important role in the study of plant development and evolution. In addition, the carrot is among the top ten vegetables grown worldwide, and the abundant orange provitamin A carotenoids that account for its familiar orange color make it the richest crop source of vitamin A in the US diet, and in much of the world. This book includes the latest genetic maps, genetic tools and resources, and covers advances in genetic engineering that are relevant for plant breeders and biologists alike.

In this book, researchers and practitioners working in the field present the major promises of algae biotechnology and they critically discuss the challenges arising from applications. Based on this assessment, the authors explore the great scientific, industrial and economic potential opened up by algae biotechnology. The first part of the book presents recent developments in key enabling technologies, which are the driving force to unleash the enormous potential of algae biotechnology. The second part of the book focuses on how practical applications of algae biotechnology may provide new solutions to some of the grand challenges of the 21st century.Algae offer great potential to support the building of a bio-based economy and they can contribute new solutions to some of the grand challenges of the 21st century. Despite significant progress, algae biotechnology is yet far from fulfilling its potential. How to unleash this enormous potential is the challenge that the own field is facing. New cultivation technologies and bioprocess engineering allow for optimization of the operation strategy of state-of the art industrial-scale production systems and they reduce the production costs. Parallel to this, new molecular technologies for genetic and metabolic engineering of (micro)algae develop quickly. The optimization of existing biochemical pathways or the introduction of pathway components makes high-yield production of specific metabolites possible. Novel screening technologies including high-throughput technologies enables testing of extremely large numbers of samples and, thus, allow for large scale modelling of biomolecular processes, which would have not been possible in the past. Moreover, profitable production can demand for integrated biorefining, which combines consecutive processes and various feedstocks to produce both transportation fuel, electric energy and valuable chemicals.

This book gathers the latest findings on the microbial ecology of saline habitats, plant-microbe interactions under saline conditions, and saline soil reclamation for agricultural use. The content is divided into four main parts: Part I outlines the definition of salinity, its genesis and impacts, and microbial diversity in saline habitats. Part II deals with impact of salinity on microbial and plant life/health. Part III highlights plant – microbe interactions in saline environments, and Part IV describes strategies for mitigation and reclamation of saline soils. The salinization of arable land is steadily increasing in many parts of the world. An excessive concentration of soluble salts (salinity) in soils or irrigation water adversely affects plant growth and survival. This problem is exacerbated in arid and semiarid areas due to their low precipitation and high evaporation rates. In turn, poor management practices and policies for using river water forthe irrigation of agriculture crops often lead to the secondary salinization of soils.Considering the growing demands of a constantly expanding population, understanding the microbial ecology and interactions under saline conditions and their implications for sustainable agriculture is of utmost importance. Providing both an essential review of the status quo and a future outlook, this book represents a valuable asset for researchers, environmentalists and students working in microbiology and agriculture..

Advancement in Crop Improvement Techniques presents updates on biotechnology and molecular biological approaches which have contributed significantly to crop improvement. The book discusses the emerging importance of bioinformatics in analyzing the vast resources of information regarding crop improvement and its practical application and utilization. Throughout this comprehensive resource, emphasis is placed on various techniques used to improve agricultural crops, providing a common platform for the utility of these techniques and their combinations. Written by an international team of contributors, this book provides an in-depth analysis of existing tools and a framework for new research. - Reviews techniques used for crop improvement, from selection and crossing over, to microorganismal approaches - Explores the role of conventional biotechnology in crop improvement - Summarizes the combined approaches of cytogenetics and biotechnology for crop improvement, including the importance of molecular techniques in this process - Focuses on the emerging role of bioinformatics for crop improvement

Soil salinity is a key abiotic-stress and poses serious threats to crop yields and quality of produce. Owing to the underlying complexity, conventional breeding programs have met with limited success. Even genetic engineering approaches, via transferring/overexpressing a single ‘direct action gene'per event did not yield optimal results. Nevertheless, the biotechnological advents in last decade coupled with the availability of genomic sequences of major crops and model plants have opened new vistas for understanding salinity-responses and improving salinity tolerance in important glycophytic crops. Our goal is to summarize these findings for those who wish to understand and target the molecular mechanisms for producing salt-tolerant and high-yielding crops. Through this 2-volume book series, we critically assess the potential venues for imparting salt stress tolerance to major crops in the post-genomic era. Accordingly, perspectives on improving crop salinity tolerance by targeting the sensory, ion-transport and signaling mechanisms are presented here in volume 1. Volume 2 will focus on the potency of post-genomic era tools that include RNAi, genomic intervention, genome editing and systems biology approaches for producing salt tolerant crops.

Cold stress is one of the prevalent environmental stresses affecting crop productivity, particularly in temperate regions. Numerous plant types of tropical or subtropical origin are injured or killed by non-freezing low temperature, and display a range of symptoms of chilling injury such as chlorosis, necrosis, or growth retardation. In contrast, chilling tolerant species thrive well at such temperatures. To thrive under cold stress conditions, plants have evolved complex mechanisms to identify peripheral signals that allow them to counter varying environmental conditions. These mechanisms include stress perception, signal transduction, transcriptional activation of stress-responsive target genes, and synthesis of stress-related proteins and other molecules, which help plants to strive through adverse environmental conditions. Conventional breeding methods have met with limited success in improving the cold tolerance of important crop plants through inter-specific or inter-generic hybridization. A better understanding of physiological, biochemical and molecular responses and tolerance mechanisms, and discovery of novel stress-responsive pathways and genes may contribute to efficient engineering strategies that enhance cold stress tolerance. It is therefore imperative to accelerate the efforts to unravel the biochemical, physiological and molecular mechanisms underlying cold stress tolerance in plants.Through this new book, we intend to integrate the contributions from plant scientists targeting cold stress tolerance mechanisms using physiological, biochemical, molecular, structural and systems biology approaches. It is hoped that this collection will serve as a reference source for those who are interested in or are actively engaged in cold stress research.

This book highlights some of the most important biochemical, physiological and molecular aspects of plant stress, together with the latest updates. It is divided into 14 chapters, written by eminent experts from around the globe and highlighting the effects of plant stress (biotic and abiotic) on the photosynthetic apparatus, metabolites, programmed cell death, germination etc. In turn, the role of beneficial elements, glutathione-S-transferase, phosphite and nitric oxide in the adaptive response of plants under stress and as a stimulator of better plant performance is also discussed. A dedicated chapter addresses research advances in connection with Capsicum, a commercially important plant, and stress tolerance, from classical breeding to the recent use of large-scale transcriptome and genome sequencing technologies. The book also explores the significance of the liliputians of the plant kingdom (Bryophytes) as biomonitors/bioindicators, and general and specialized bioinformatics resources that can benefit anyone working in the field of plant stress biology. Given the information compiled here, the book will offer a valuable guide for students and researchers of plant molecular biology and stress physiology alike.

The natural environment for plants is composed of a complex set of abiotic and biotic stresses; plant responses to these stresses are equally complex. Systems biology allows us to identify regulatory hubs in complex networks. It also examines the molecular “parts” (transcripts, proteins and metabolites) of an organism and attempts to combine them into functional networks or models that effectively describe and predict the dynamic activities of that organism in different environments. This book focuses on research advances regarding plant responses to abiotic stresses, from the physiological level to the molecular level. It highlights new insights gained from the integration of omics datasets and identifies remaining gaps in our knowledge, outlining additional focus areas for future crop improvement research. Plants have evolved a wide range of mechanisms for coping with various abiotic stresses. In many crop plants, the molecular mechanisms involved in a single type of stress tolerance have since been identified; however, in order to arrive at a holistic understanding of major and common events concerning abiotic stresses, the signaling pathways involved must also be elucidated. To date several molecules, like transcription factors and kinases, have been identified as promising candidates that are involved in crosstalk between stress signalling pathways. However, there is a need to better understand the tolerance mechanisms for different abiotic stresses by thoroughly grasping the signalling and sensing mechanisms involved. Accordingly, this book covers a range of topics, including the impacts of different abiotic stresses on plants, the molecular mechanisms leading to tolerance for different abiotic stresses, signaling cascades revealing cross-talk among various abiotic stresses, and elucidation of major candidate molecules that may provide abiotic stress tolerance in plants.

This book examines the utilization of algae for the development of useful products and processes with the emphasis towards green technologies and processes, and the requirements to make these viable. Serving as a complete reference guide to the production of biofuels and other value added products from micro and macro algae, it covers various aspects of algal biotechnology from the basics to large scale cultivation, harvesting and processing for a variety of products. It is authored and edited by respected world experts in the field of algal biotechnology and provides the most up to date and cutting edge information on developments in the field. Over the past decade there has been substantial focus and related literature on the application of algal biomass for the generation of novel processes and products. ‘Algae Biotechnology: Products and Processes'encompasses a holistic approach to critically evaluating developments in the field of algal biotechnology whilst taking into account recent advances and building on the body of knowledge. Aspects of the effects of harmful algae are also discussed, as well as the potential commercial application of algal biotechnology, the techno-economic feasibility of algal biodiesel production and the use of genetic and metabolic engineering for the improvement of yield. Other bioenergy sources such as alcohol fuels, aviation fuels, biohydrogen and biogas are also covered. This book is intended for postgraduates and researchers working in the biofuels and algal industry; it constitutes ideal reference material for both early stage and established researchers.

This book covers the state-of-the-art of microalgae physiology and biochemistry (and the several –omics). It serves as a key reference work for those working with microalgae, whether in the lab, the field, or for commercial applications. It is aimed at new entrants into the field (i.e. PhD students) as well as experienced practitioners. It has been over 40 years since the publication of a book on algal physiology. Apart from reviews and chapters no other comprehensive book on this topic has been published. Research on microalgae has expanded enormously since then, as has the commercial exploitation of microalgae. This volume thoroughly deals with the most critical physiological and biochemical processes governing algal growth and production.

This book describes the strategy used for sequencing, assembling and annotating the tomato genome and presents the main characteristics of this sequence with a special focus on repeated sequences and the ancestral polyploidy events. It also includes the chloroplast and mitochondrial genomes. Tomato (Solanum lycopersicum) is a major crop plant as well as a model for fruit development, and the availability of the genome sequence has completely changed the paradigm of the species'genetics and genomics. The book describes the numerous genetic and genomic resources available, the identified genes and quantitative trait locus (QTL) identified, as well as the strong synteny across Solanaceae species. Lastly, it discusses the consequences of the availability of a high-quality genome sequence of the cultivated species for the research community. It is a valuable resource for students and researchers interested in the genetics and genomics of tomato and Solanaceae.

Biotechnology is the technical application that uses living organisms or biological systems to make products that have a profound impact on agriculture, environment, and human health.In this text book, a color-coded classification is used to present basic chapters on white, red, green and blue biotechnology. Beside traditional biotechnical processes, the book will address principles of modern biotechnology research and applications.Each chapter has a general introduction and concluding paragraph, gives key terms, will address problems, and recommends additional readings.This text book is ideally suited for advanced graduate or master students and will also be a good reference for PhD students, physicians, engineers, attorneys, or non-specialist with an interest into biotechnology.

This book provides detailed and comprehensive information on oxidative damage caused by stresses in plants with especial reference to the metabolism of reactive oxygen species (ROS).In plants, as in all aerobic organisms, ROS are common by-products formed by the inevitable leakage of electrons onto O2 from the electron transport activities located in chloroplasts, mitochondria, peroxisomes and in plasma membranes or as a consequence of various metabolic pathways confined in different cellular loci. Environmental stresses such as heat, cold, drought, salinity, heavy-metal toxicity, ozone and ultraviolet radiation as well as pathogens/contagion attack lead to enhanced generation of ROS in plants due to disruption of cellular homeostasis. ROS play a dual role in plants; at low concentrations they act as signaling molecules that facilitate several responses in plant cells, including those promoted by biotic and abiotic agents. In divergence, at high levels they cause damage to cellular constituents triggering oxidative stress. In either case, small antioxidant molecules and enzymes modulate the action of these ambivalent species.

In this book, authors who are experts in their fields describe current advances on commercial crops and key enabling technologies that will underpin future advances in biotechnology. They discuss state of the art discoveries as well as future challenges. Tremendous progress has been made in introducing novel genes and traits into plant genomes since the first creation of transgenic plants thirty years ago, and the first commercialization of genetically modified maize in 1996. Consequently, cultivation of biotech crops with useful traits has increased more than 100-fold from 1.7 million hectares in 1996 to over 175 million hectares globally in 2013. This achievement has been made possible by continued advances in understanding the basic molecular biology of regulatory sequences to modulate gene expression, enhancement of protein synthesis and new technologies for transformation of crop plants.This book has three sections that encompass knowledge on genetically modified (GM) food crops that are currently used by consumers, those that are anticipated to reach the market place in the near future and enabling technologies that will facilitate the development of next generation GM crops. Section I focuses only on genetically modified maize and soybean (3 chapters each), while Section II discusses the GM food crops rice, wheat, sorghum, vegetables and sugar cane. Section III covers exciting recent developments in several novel enabling technologies, including gene targeting, minichromosomes, and in planta transient expression systems.

Plastids are the sites of conversion of solar energy into the chemical energy usable to sustain life. They are also responsible for the production of the vast majority of the oxygen in the atmosphere. Through these activities they play a unique role in the biosphere, producing two critical products upon which life on Earth depends. It covers in 21 chapters nearly all actively investigated areas of plastid biology, from biosynthesis to function to their uses in biotechnology. The editors have compiled an extensive list of international experts from whom to solicit chapters. As is evident from the suggested Table of Contents, the book will start with a discussion of genetic material and its expression, followed by differentiation and development of different plastid types and internal organization. This is followed by an in depth look at biogenesis and assembly of plastid proteins and protein complexes and then by the important metabolic functions in plastids. The book will end with two chapters discussing the role of plastid biology in protein expression biotechnology and in hydrogen and biofuel production.

The bioactive compounds of plants have world-wide applications in pharmaceutical, nutraceutical and food industry with a huge market. In this book, a group of active researchers have addressed on the most recent advances in plant cell and organ cultures for the production of biomass and bioactive compounds using bioreactors.Tremendous efforts have been made to commercialize the production of plant metabolites by employing plant cell and organ cultures in bioreactors. This book emphasizes on the fundamental topics like designing of bioreactors for plant cell and organ cultures, various types of bioreactors including stirred tank, airlift, photo-bioreactor, disposable bioreactor used for plant cell and organ cultures and the advantages and disadvantages of bioreactor cultures. Various strategies for biomass production and metabolite accumulation have been discussed in different plant systems including Korean/Chinese ginseng, Siberian ginseng, Indian ginseng, Echinacea, St. John's wort, Noni, Chinese licorice, Caterpillar fungus and microalgae. Researches on the industrial application of plant cells and organs with future prospects as well as the biosafety of biomass produced in bioreactors are also described. The topics covered in this book, such as plant cell and organ cultures, hairy roots, bioreactors, bioprocess techniques, will be a valuable reference for plant biotechnologists, plant biologists, pharmacologists, pharmacists, food technologists, nutritionists, research investigators of healthcare industry, academia, faculty and students of biology and biomedical sciences. The multiple examples of large-scale applications of cell and organ cultures will be useful and significant to industrial transformation and real commercialization.

A comprehensive and mechanistic perspective on fruit ripening, emphasizing commonalities and differences between fruit groups and ripening processes. Fruits are an essential part of the human diet and contain important phytochemicals that provide protection against heart disease and cancers. Fruit ripening is of importance for human health and for industry-based strategies to harness natural variation, or genetic modification, for crop improvement. This book covers recent advances in the field of plant genomics and how these discoveries can be exploited to understand evolutionary processes and the complex network of hormonal and genetic control of ripening. The book explains the physiochemical and molecular changes in fruit that impact its quality, and recent developments in understanding of the genetic, molecular and biochemical basis for colour, flavour and texture. It is a valuable resource for plant and crop researchers and professionals, agricultural engineers, horticulturists, and food scientists. Summary: Reviews the physiochemical and molecular changes in fruit which impact flavour, texture, and colour Covers recent advances in genomics on the genetic, molecular, and biochemical basis of fruit quality Integrates information on both hormonal and genetic control of ripening Relevant for basic researchers and applied scientists

Crops experience an assortment of environmental stresses which include abiotic viz., drought, water logging, salinity, extremes of temperature, high variability in radiation, subtle but perceptible changes in atmospheric gases and biotic viz., insects, birds, other pests, weeds, pathogens (viruses and other microbes). The ability to tolerate or adapt and overwinter by effectively countering these stresses is a very multifaceted phenomenon. In addition, the inability to do so which renders the crops susceptible is again the result of various exogenous and endogenous interactions in the ecosystem. Both biotic and abiotic stresses occur at various stages of plant development and frequently more than one stress concurrently affects the crop. Stresses result in both universal and definite effects on plant growth and development. One of the imposing tasks for the crop researchers globally is to distinguish and to diminish effects of these stress factors on the performance of crop plants,especially with respect to yield and quality of harvested products. This is of special significance in view of the impending climate change, with complex consequences for economically profitable and ecologically and environmentally sound global agriculture. The challenge at the hands of the crop scientist in such a scenario is to promote a competitive and multifunctional agriculture, leading to the production of highly nourishing, healthy and secure food and animal feed as well as raw materials for a wide variety of industrial applications. In order to successfully meet this challenge researchers have to understand the various aspects of these stresses in view of the current development from molecules to ecosystems. The book will focus on broad research areas in relation to these stresses which are in the forefront in contemporary crop stress research.

With the high interest in renewable resources, the field of algal biotechnology has undergone a huge leap in importance in recent years. The book Microalgae Biotechnology - Integration and Economy treats integrated approaches to bring the high potential of microalgae into application, accelerate the development of really working production processes and put finally the products on the market. Close interaction of biology and process engineering becomes visible in the described processes. The big impact of microalgal biotechnology on our future society is outlined as a desirable consequence of scientific progress. This book will allow protagonists in academia and industry as well as decision makers in industry and politics to get a clear picture of current possibilities and future trends in microalgal biotechnology.

Mitochondria are the product of a long evolutionary history. It is now a well established fact that mitochondria did evolve from free living bacteria being the common ancestor of both, eukaryotic mitochondria and α-proteobacteria. Advances in genome sequencing, the establishment of in organello and in vitro assays to name only a few, contributed significantly to advances in plant mitochondrial research. Second generation sequencing and the ability to directly sequence and analyse the whole plant transcriptome certainly will help to develop the research on plant mitochondria to another level in the future. In this book the current knowledge about plant mitochondria is presented in a series of detailed chapters, which have been organized in five main sections: (i) dynamics, genes and genomes; (ii) transcription and RNA processing; (iii) translation and import; (iv) biochemistry, regulation and function; and (v) mitochondrial dysfunction and repair. These sections consist of two to five chapters, each written by well-known specialists in the field. This book thus provides a comprehensive inside in the field of plant mitochondria for the specialist. The addition of a glossary and text boxes to each chapter provides easy access for readers from other subjects and hopefully will attract young scientist to the fascinating and exiting field of plant mitochondria.

Understanding the mechanisms whereby plant development is regulated is crucial for crop improvement using genetic engineering. This work, comprising two volumes, reviews recent advances in plant developmental biology and explores the possibility of their practical applications from biotechnological perspectives. Volume 1 deals with the plant model and its life cycle. Topics include the formation of shoots, roots, flowers and gametes, pollen germination, fertilization, fruit development and ripening, seed development, dormancy and germination, apomixis, male sterility and self-incompatibility. Volume 2 focuses on development-related areas, including tissue culture (somatic embryogenesis, microspore embryogenesis, somaclonal variation), plant processes (photosynthesis, seed maturation and seed proteins, fatty acids, vitamins, alkaloids, flower pigments and scent), signalling (amino compound-containing lipids, auxins, cytokinins and light), and molecular genetics of developmental regulation (RNA silencing, DNA methylation, epigenetics, activation tagging, homologous recombination and synthetic promoter engineering). This work is a key reference for plant breeders, researchers and graduate students in the fields of plant biotechnology, agronomy, horticulture, genetics and functional genomics, and cell and molecular biology.

The past two decades have witnessed an enormous progress in our understanding of plastid (chloroplast) biology. Basic principles of plastid biogenesis, genome structure and function, gene expression and its regulation as well as plastid-nuclear interaction and communication pathways have been elucidated. In addition, the possibility to directly alter the genetic information of the plastid by transformation has facilitated the study of virtually all aspects of plastid biology in vivo and, moreover, has paved the way to diverse applications of transgenic plastids in biotechnology. The present book provides a comprehensive overview of our current knowledge on plastid biogenesis, plastid-nuclear communication, the regulation of plastid gene expression at all levels, and also assesses the state of the art in key technologies, such as proteomics and chloroplast transformation. Written by recognized experts in the field, it covers plastid differentiation and division, genome structure and function, plastid inheritance, recombination, DNA replication and repair, transcription, RNA processing and translation as well as crucial posttranslational processes in plastid biogenesis and function, including protein processing, the assembly of multiprotein complexes, protein stability and degradation, protein import and sorting.

Genome Mapping and Molecular Breeding in Plants presents the current status of the elucidation and improvement of plant genomes of economic interest. The focus is on genetic and physical mapping, positioning, cloning, monitoring of desirable genes by molecular breeding and the most recent advances in genomics. The series comprises seven volumes: Cereals and Millets; Oilseeds; Pulses, Sugar and Tuber Crops; Fruits and Nuts; Vegetables; Technical Crops; and Forest Trees. Vegetables contains reviews in 12 chapters contributed by 31 authors from 10 countries. Covered are tomato, lettuce, radish, carrot, beet, eggplant, cucurbits, onion, garlic and other crops included in Brassica rapa, Brassica oleracea, capsicums and cucurbits. The impressive work that has been done on most of these crops is presented in this volume. Genome projects already initiated on vegetable crops, particularly on Solanaceae and Brassicaceae species, may ignite further interest in other vegetables as well.