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Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. - Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish - Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text - Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins - Includes more sample problems in every chapter for readers to practice concepts

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts

Calculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. Mathematics, as a vital component of the successful design and interpretation of basic research, is used daily in laboratory work. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis.•A comprehensive guide to calculations for a wide variety of problems encountered in the basic research laboratory.• Example calculations are worked through from start to finish in easy-to-follow steps• Key chapters devoted to calculations encountered when working with bacteria, phage, PCR, radioisotopes, recombinant DNA, centrifugation, oligonucleotides, protein, and forensic science.•Written for students and laboratory technicians but a useful reference for the more experienced researcher.•A valuable teaching resource.

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.Key Features• Includes a glossary of over 200 terms important for understanding molecular biology• Uses an inexpensive source of eukaryotic cells - great for schools on a budget• Includes Methods Locator that provides instant access to the latest methods• Contain clearly written, easy-to-follow, student-tested instructions:• Sterile techniques• Phage titration• Gel electrophoresis of DNA• Restriction enzyme digestion• Plasmid isolation• Transformation of E. Coli• Recombinant DNA cloning• Nick translation labeling • Nonradioactive primer labelling• Nonradioactive DNA detection• Southern blotting• Colony hybridization• Purification of plant DNA• RNA purification• Northern blotting• Purification of poly A+ RNA• Polymerase chain reaction (PCR)

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! - Concepts explained - Methods detailed - Trouble-shooting emphasized - Novel applications highlighted - Key concepts for PCR - Analysis of PCR products - Research applications - Alternative amplification strategies