1. Sustained-Release Synthetic Biomarkers for Monitoring Thrombosis and Inflammation Using Point-of-Care Compatible Readouts.
- Author
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Dudani, Jaideep S., Buss, Colin G., Akana, Reid T. K., Kwong, Gabriel A., and Bhatia, Sangeeta N.
- Subjects
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CONTROLLED release preparations , *BIOMARKERS , *THROMBOSIS , *INFLAMMATION , *DISEASE management , *POLYETHYLENE glycol - Abstract
Postoperative infection and thromboembolism represent significant sources of morbidity and mortality but cannot be easily tracked after hospital discharge. Therefore, a molecular test that could be performed at home would significantly impact disease management. The laboratory has previously developed intravenously delivered 'synthetic biomarkers' that respond to dysregulated proteases to produce a urinary signal. These assays, however, have been limited to chronic diseases or acute diseases initiated at the time of diagnostic administration. Here, a subcutaneously administered sustained-release system, using small poly(ethylene glycol) scaffolds (<10 nm) to promote diffusion into the bloodstream over a day, is formulated. The utility of a thrombin sensor to identify thrombosis and an Matrix metalloproteinase (MMP) sensor to measure inflammation is demonstrated. Finally, a companion paper ELISA (Enzyme-linked immunosorbent sssay), using printed wax barriers, with nanomolar sensitivity for urinary reporters for point-of-care detection is developed. The approach for subcutaneous delivery of nanosensors combined with urinary paper analysis may enable facile monitoring of at-risk patients. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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