1. Treatment of pulp and paper mill effluent by a novel bacterium Bacillus sp. IITRDVM-5 through a sequential batch process.
- Author
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Sonkar, Madan, Kumar, Madan, Dutt, Dharm, and Kumar, Vivek
- Subjects
PAPER pulp ,PAPER mills ,PULP mills ,BACILLUS (Bacteria) ,BATCH processing ,LIGNINS ,WATER filters - Abstract
The various types of biorefractory organic pollutants present in pulp and paper mill effluent is obnoxious to the environment and severely affects the biotic and abiotic components. The present study investigates the isolation and characterization of a novel strain isolated from pulp and paper mill effluent. Among the isolates, the strain Bacillus sp. IITRDVM-5 showed maximum degradation and decolorization of 100% filter sterilized effluent, supplemented with only nitrogen and phosphorus in a sequential batch treatment. Under optimized conditions, the maximum reduction in TOC, COD, BOD and color was 82.22, 89.50, 93.33 and 73.01%, respectively after 72 h treatment. In addition, the reduction in total phenol, AOX and lignin was 88.5, 75.63 and 64.10%, respectively. The UV–Vis spectral scan identified the degradation pattern of contaminants during treatment. FTIR and GC-MS analysis indicated significant change in functional groups composition of organic pollutants and confirmed their transformation after treatment. Significant degradation of phenolic and chlorolignin derivatives was observed through GC-MS. Furthermore, the evaluation of toxicity with V. radiata and T. foenum-graecum seeds confirmed the toxic nature of effluent, of which detoxification was observed post bacterial treatment. The results highlight the degradation and decolorization potential of Bacillus sp. IITRDVM-5 and its future application in pulp and paper effluent treatment. Display Omitted • The paper mill effluent treatment was done without addition of buffer or additional carbon source. • 88.5, 75.63, 64.10, 82.22 and 73.01% reduction in total phenol, AOX, lignin, TOC and color was observed. • UV–Vis scan, FTIR and GC-MS confirmed the degradation of lignin and chlorolignin derivatives. • Detoxification of effluent post treatment was confirmed by germination in V. radiata and T. foenum-graecum seeds. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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