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Bacterial levels in Buffalo Bayou in Houston commonly exceed contact recreation standards. Potential sources of bacteria include wastewater treatment plants, sanitary sewer overflows, septic systems, wet and dry nonpoint-source discharges via direct runoff and pipes, direct deposition, and sediment. A water-quality model in the Hydrologic Simulation Program-FORTRAN (HSPF) was calibrated and validated for hydrology, sediment, and Escherichia coli and subsequently used to evaluate the impacts of the bacterial sources in the watershed. In addition, simple estimates of bacterial loads were calculated along with source evaluations from load duration curves. Load reductions based upon the simple estimates indicated that water-quality standards were met by reducing dry-weather indicator bacterial loads by 69% and wet-weather loads by 98%. When these load reductions were implemented in the HSPF model, however, standards were not met under dry-weather conditions. Residual nonpoint-source loading was found to cause the discrepancy between simple load estimate calculations and the developed water-quality model. This paper demonstrates that runoff can play a significant role in maintaining high levels of bacteria under all flow conditions and that understanding the temporal variations in bacterial source loading is critical to ensure that load reductions will achieve water-quality standards. [ABSTRACT FROM AUTHOR]

The model developed in this paper, the bacteria loading estimator spreadsheet tool (BLEST), was designed as an easy to use indicator bacteria model that can overcome the shortcomings of many of the simpler total maximum daily load (TMDL) modeling approaches by integrating spatial variation into load estimates. BLEST was applied to the Buffalo Bayou watershed in Houston, Texas and incorporated loading from point and nonpoint sources, such as wastewater treatment plants, sanitary sewer overflows, septic systems, storm sewer leaks, runoff, bed sediment resuspension, and direct deposition. The dry weather Escherichia coli load in Buffalo Bayou was estimated using BLEST to be 244 billion MPN/day and would require an overall 48% reduction to meet the contact recreation standard, while wet weather loads would need to be reduced by 99.7%. Dry weather loads were primarily caused by animal direct deposition, septic systems and discharges from storm sewers under dry weather conditions, while wet weather loads were mostly attributable to runoff and resuspension from sediment. Unlike most simple TMDL load allocation strategies, BLEST can be used to evaluate spatially variable load reduction strategies. For example, septic system load reductions implemented in less than 10% of the subwatersheds resulted in a decrease in bayou loading of more than 20%. [ABSTRACT FROM AUTHOR]