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It is now common knowledge that many water resources stresses relate to access to water within a basin. Yi River Basin, a typical river basin characterized by intensive agricultural processes, significant population growth, and water management, has been undergoing grave water problems. In this paper, the long-term trend of precipitation and streamflow in Yi River Basin, from 1964 to 2010, was investigated via Mann-Kendall test. The change point occurred in the year 1965 dividing the long-term series into two periods. Climate elasticity method and linear regression method were implemented to quantify the impact of precipitation and human activities on runoff and presented basically consistent results of the percentage change in an annual runoff for the postchange period. The results reveal that the decline of annual runoff in postchange period is mainly attributed to precipitation variability of 53.66–58.25% and human activities of 46.34–41.74%, as estimated by climate elasticity method and linear regression method, respectively. This study detected the changes in the precipitation-streamflow relationship and investigated the possible causes in the Yi River, which will be helpful for providing a reference for the management of regional water resources. [ABSTRACT FROM AUTHOR]