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Through the use of paper chromatographic separation, and fluorometric and spectrophotometric analysis, three caffeoylquinic acid isomers and scopolin have been quantitated in tobacco as a function of age and chilling temperature. Cold-chilled plants exhibited nitrate deficiency symptoms and 4-5 fold increases over control plants in chlorogenic acid (CGA) concentration in all plant sections except the roots. Varying lesser increases in neochlorogenic acid, "Band 510", and scopolin concentration were also observed in the above ground sections of chilled Plants. hoots of cold-chilled plants contained lesser concentrations of CGA and scopolin than control plants after 15 days of treatment. Leaves from the same control plant were found to decrease in CGA, Band 510 and scopolin concentration with age, while neochlorogenic acid concentrations changed little. In stem sections CGA concern ration also decreased basipetally while the concentrations of scopolin increased. Similar leaf age studies done with cold -chilled plants revealed phenolic concentration changes comparable to those of control plants, even though their concentrations were appreciably higher. [ABSTRACT FROM AUTHOR]