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Introduction The number of people who are considered to be either overweight or obese continues to increase, worldwide. The aim of the paper was to determine the influence of overweight on the quality of life of health workers. Material and methods The study of prevalence was used. We observed 108 health workers both men and women from the Institute for Public Health in the City of Niš. The questionnaire Obesity Related Well-Being 97 (ORWELL97) was applied in all health workers. Results A hundred and five health workers filled in the ORWELL97 questionnaire. There were 40% of men and 60% of women; 72% of them were married and 28% of them were single; 68% were from the urban area and 32% were from the rural area. The average age was 29.5 (SD=3.2) and the average value of BMI was 25.4 (SD=4, 0). There were 54 (48.6%) health workers having normal weight and there were 51(5.4%) who were considered overweight. No one was obese among them The difference between the average sum of scores in the group of health workers who had normal weight and the group of health workers who were overweight was not statistically significant (t=1.1 i p<0.05). Nor was the difference statistically significant for the average sum of scores of subquestion O (t=3.6 and p<0.05), and subquestion R (t=2.9 and p<0.05). BMI showed a weak positive correlation with both occurrence and relevance of symptoms. A weak positive correlation of BMI was found with average sum of scores in the first, second and third groups of questions in ORWELL 97 questionnaire. Conclusion According to the presented results, health workers who were overweight did not have lower quality of life than health workers who had normal weight. [ABSTRACT FROM AUTHOR]