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[Objective] The present paper aimed to identify CBF family members using whole genome data of Prunus sibirica, and to analyze the characteristics and functions of the conserved domains and their expression patterns after low temperature stress of CBF transcription fac- tors. [Method] By the P. sibirica whole genome database, the CBF genes of P. sibirica were searched using BLASTP and HMM, and their conserved structural domains were verified by CDD and MEME. Bioinformatics tools such as Prot-Param, WOLF PSORT, MAGA X, Phyre2 and PlantCARE were used to analyze protein physicochemical properties, subcellular localization, phylogeny, tertiary structure, chromosom- al localization and cis-elements, respectively. Quantitative real-time PCR (RT-qPCR) was used to analyze the expression pattern of CBF gene after low temperature stress in P. sibirica. [Result] In the study, four PSCBF's were identified, all of which possessed the conserved structural domains PKKPAGR and DSAWR sequences of the AP2 gene family. The PSCBFs contained 206-246 amino acids, with a mean molecular weight of 25. 80 KDa and a mean isoelectric point of 5.92. PSCBF3 and PSCBF4 were similar in gene structure and had closer ge- netic relationship. The subcellular localization of 4 PSCBFs was located in the nucleus. Promoter cis-acting element analysis showed that CBF family members could participate in plant hormone and abiotic stress responses. Four CBF members were all acidic and hydrophilic pro- teins. The expression of PSCBF's was higher mainly in the early stage (15 min) after low temperature stress. [Conclusion] The study shows more complete information on the CBF gene family of P. sibirica, which offers new insights into the complex mechanisms and pathways of PSCBF in response to low-temperature stress at different stages. It lays an important theoretical foundation for further investigations into the biological functions of CBF family members. [ABSTRACT FROM AUTHOR]

【Objective】The paper aimed to effectively solve the problem of pollen pollution and cultivate male sterile Lilium using molecular biology technology. The role of ATPase3 gene, a member of the ATPase family, in the male sterility Lilium was studied, which would provide a theoretical basis for the application of this gene in the pollen-free molecular breeding of Lilium.【Method】Anthers of fertile lines and sterile lines of Liliums were used as experimental materials, and a significantly up-regulated ATPase3 gene was screened from the results of transcriptome sequencing of fertile lines. Relative expression of ATPase3 gene in different parts of Lilium was detected by fluorescence quantitative qRT-PCR during the period of microsporocyte. Full-length cDNA sequence of anther ATPase3 gene of Lilium was amplified by qRT-PCR and Race technology, and the structure and physicochemical properties of Lilium ATPase3 were analyzed by various online bioinformatics software（Prot Param, Prot Scale, NCBI Conserved Domain, SWISS-MODEL）. 【Result】The expression of ATPase3 gene in Lilium was the highest in flower organs, and the expression in anthers of fertile lines was 7 times higher than that of sterile lines. Full-length ORF of Lilium ATPase3 gene was 327 bp, encoding 109 amino acids and containing a typical conserved ATPase3 domain; Relative molecular weight of the protein was 12.14 KD and theoretical isoelectric point was 7.71. It was a hydrophilic protein with transmembrane structure, and the secondary and tertiary structures of the protein were predicted. Bioinformatics analysis showed that the predicted protein had typical characteristics of ATP synthase. Homologous sequence analysis showed that the protein had the closest homology with Arabidopsis thaliana plasma membrane ATPase3 and H[+]ATPase1, H[+]ATPase2, H[+]ATPase3, H[+]ATPase8. This protein was a plasma membrane ion transporter that provided energy in plants. It was speculated that the expression level of this protein was down-regulated in the anthers of Lilium sterile lines, which led to the male sterility of Lilium.【Conclusion】In the study, ATPase3 gene was verified to be related to male sterility in Lilium, and ATPase3 gene was successfully cloned and analyzed by bioinformatics. It provided a theoretical basis for the occurrence of male sterility in Lilium caused by the loss of plasma membrane ion transporter function which was encoded by ATPase3 gene. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to identify and analyze the KUP/HAK/KT gene family in sweet potato and provided a theoretical basis for further study on the function of IbHAK gene. 【Method】Based on bioinformatics, the KUP/HAK/KT gene family of sweet potato was identified at the whole genome level by the genome data of Taizhong No.6, and the basic characteristics, phylogenetic evolution, gene replication and expression patterns of family members were further analyzed. 【Result】A total of 22 IbHAK genes were identified in sweet potato. The length of IbHAK protein ranged from 227 aa to 1252 aa, the molecular weight ranged from 18 297.95 to 140 640.46 u, and the isoelectric point ranged from 5.54 to 9.31, all IbHAK genes were located on the plasma membrane, and the complete secondary structure of IbHAK protein had 4 to 13 transmembrane regions. According to the phylogenetic relationship, it could be divided into four evolutionary clusters: Cluster Ⅰ, Cluster Ⅱ, Cluster Ⅲ and Cluster Ⅳ. Chromosome mapping showed that 22 genes were distributed on 11 chromosomes, among which Chr4 chromosome was the most distributed, with 4 IbHAK genes, and 8 replication genes were produced by 22 IbHAK genes. All of the 22 IbHAK genes contained TATA and CAAT elements related to plant growth and development as well as light-responsive elements. All of the IbHAK genes contained at least one stress-responsive element and one hormone-responsive element. IbHAK genes showed tissue expression specificity, among which IbHAK6, IbHAK8, IbHAK9 and IbHAK22 had the highest expression levels in roots, and the expression levels of many IbHAK genes changed under stress. 【Conclusion】Twenty-two KUP/HAK/KT gene family members were identified from the whole genome of sweet potato. IbHAK6, IbHAK8, IbHAK9 and IbHAK22 gene family members were conserved in evolution. Most of IbHAK genes were able to respond to stress. [ABSTRACT FROM AUTHOR]