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To solve the environmental pollution and harm to human health caused by the use of adhesives in traditional paperboard, the development of environmentally friendly mycelium paperboard has become a hot topic. Due to the high dipole moment and hydrogen bonding between macromolecules, hyphae can form clamp shaped connection structures for nutrient reproduction and transfer, and can grow on natural agricultural waste, forming a natural biocomposite material. This study determined the combination of Ganoderma weberianum/corn straw, and designed single factor cultivation conditions such as cultivation time, straw particle size, and fructose addition to explore the effects on the mechanical properties, waterproof properties, and thermal decomposition performance of the hyphal paperboard formed by hot pressing. The optimal cultivation process was obtained through orthogonal experiments. The experimental results indicate that various factors have varying degrees of influence on the edgewise strength, flexural stiffness, and static bending strength and waterproofness of paperboard. Firstly, when the cultivation time was 30 days, the flexural stiffness reached 16.63 mN/m, which was 21.2% higher than the flexural stiffness of kraft paper (13.10 mN/m). Moreover, with the increase of cultivation time, the water absorption (24 hours) had also decreased from 3.92% to 1.27%. Secondly, previous research results have shown that the addition of fructose, glucose, and sucrose increases the biomass growth rate of strains. In this study, fructose was chosen as an exogenous nutrient, and the results showed that when a mass fraction of 5% fructose was added, the mechanical properties were the best, with a flexural stiffness of 18.87 mN/m, which was 30.5% higher than that of kraft paper (13.10 mN/m). Thirdly, the particle size of the sbstrate has a significant impact on the appearance and properties of the material. The results show that, from the appearance perspective, the mycelium paperboard made of 0.165～0.250 mm particle size has the closest appearance and feel to kraft paper. At the same time, during the testing period, it was found that the mycelium paperboard made of 0.165～0.250 mm straw was the least prone to deformation and loosening, and the molding effect was significantly improved compared to other components. In terms of mechanical properties, the edgewise strength increased by 17.4%, the static bending strength increased by 14.9%, and the flexural stiffness increased by 57.3%. The experimental results show that the degree of influence of various factors on the edgewise strength, flexural stiffness, and static bending strength of paperboard is in the order of particle size, cultivation time,fructose addition amount. When the particle size is 0.165～0.250 mm, the cultivation time is 30 days, and the fructose addition of 5.0%, the edgewise strength of the prepared paperboard is 14.36 N/mm, the static bending strength is 10.89 MPa, and the flexural stiffness is 33.09 mN/m. As the particle size decreases, the water absorption rate and water thickness expansion rate of the paperboard after 24 hours of water absorption decrease to 0.86% and 0.59%. The mycelium paperboard prepared by different single factors has the same thermal decomposition process, starting at around 150 ℃ and reaching its maximum at 350 ℃. This study provides a reference basis for the development and application of mycelium composite materials in the future. [ABSTRACT FROM AUTHOR]

Banana fusarium wilt has a significant adverse effect on the banana industry. It is worth noting that fusarium oxysporum f.sp. cubense 4(Foc4) is one of the most devastating and widespread pathogens of banana fusarium wilt in the world. It is a catastrophic threat to the global banana industry. At present, there is still no technology that can cure bananas infected with banana fusarium wilt. Therefore, it is extremely important for banana planting industry to detect banana fusarium wilt in time and do a good job in prevention and control. In order to realize the early accurate detection of banana fusarium wilt race 4, this study proposed a detection method of double probe paper-based sensor based on colloidal gold, which increased the use of signal enhancement probes to increase the binding amount of colloidal gold, thereby reducing the detection limit. Two kinds of colloidal gold particles with different particle sizes were combined with the corresponding detection probes and signal enhancement probes to form a double gold label probe system. The large particle size colloidal gold was used with the detection probe, while the small particle size colloidal gold was paired with the signal enhancement probe. The sample being tested was mixed with this double gold-labeled probe system. The Foc4 target sequence in the sample would be paired with the double gold-labeled probe to form a ' gold-labeled probe-target sequence-T-line probe ' complex, which continued to migrate to the water-absorbing pad. The complex was captured on the test area of the sensor. Within 10 minutes, the test paper would generate a visible target product that could be observed by the naked eye at both the test line and the quality control line areas, thus achieving qualitative detection. For quantitative detection of Foc4, the light intensity of the test strip would be further analyzed using software. The light intensity peak area was obtained and substituted into the standard curve to obtain the concentration of Foc4 detected and realize quantitative detection. Experimental results demonstrate that the detection limit of the dual-probe paper-based sensor is 0.001 nmol/L, which is 100 times of that of the traditional paper-based sensor, and the detection sensitivity is improved. The sensitivity test results demonstrated a strong linear relationship between the concentration of Foc4 and the peak area of the test line light intensity within the concentration range of 0.001-1 000.000 nmol/L. This finding suggests that the sensor can be effectively employed for quantitative detection. To assess specificity, a high concentration of non-complementary probe was utilized as the target sequence for detection and compared with the detection results of samples containing Foc4. The experiment revealed the absence of a red band at the test line when the test paper was detected by the noncomplementary sequence. Importantly, this observation indicated that the presence of the non-complementary sequence had minimal impact on the detection effect, thus affirming the sensor's excellent specificity. Finally, the paper-based sensor was used to detect Foc4 in banana leaves. The average recovery rate was 77.6%-102.3%, and the relative standard deviation was 7.4%-7.7%, indicating that it can be used for on-site detection of Foc4. The detection technology possesses the characteristics of being low-cost and easy to operate. In comparison to traditional detection methods, it enables timely, rapid and accurately determination of the presence of Foc4. Compared with the existing molecular detection technology, the detection can be completed without the need of expensive equipment or specialized personnel. This feature makes it highly suitable for widespread use. The dual-probe paper-based sensor realizes the combination of nucleic acid lateral chromatography detection technology and banana fusarium wilt detection. Its potential for widespread adoption in the early disease warning system of the banana industry is substantial. Furthermore, this technology can be combined with traditional polymerase chain reaction (PCR) and other techniques to facilitate on-site detection of Foc4. [ABSTRACT FROM AUTHOR]

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Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Heat exchangers serve as pivotal components in heat exchange systems. Compared with the straight channels, spiral channels have better performance due to the curvature of the centrifugal force generated by the secondary flow. Consequently, heat exchangers employing spiral channels find widespread application in agricultural engineering, including greenhouse air conditioning, water circulation systems, and agricultural product processing and storage for effective heat management. In this paper, we propose enhancing the heat transfer efficiency of spiral channels by introducing corrugations with longitudinal fins on the outer channel wall. To elucidate the impact of corrugation, we establish three-dimensional models of smooth spiral channels, corrugated spiral channels, and corrugated spiral channels with longitudinal fins. Initially, we investigate the influence of corrugated number on flow and heat transfer performance numerically. Through comparative analysis involving the development and variation of velocity and vorticity fields, average Nusselt number Nu, fanning friction factor f, average Dean number Dnm, secondary flow intensity Se under different corrugated numbers, and the Performance Evaluation factor of heat exchanger based on Comprehensive (PEC0), insights are drawn. Subsequently, longitudinal fins are integrated into the corrugated spiral channel, and a numerical study is conducted to assess the effect of varying geometric parameters of the longitudinal fin on the fluid flow and heat transfer performance inside the channel. Analysis of different longitudinal fin widths and heights on velocity and temperature fields, Nu, f, and Performance Evaluation factor of heat exchanger based on Comprehensive (PECw) of the channel is performed. The findings indicate that corrugated spiral channels outperform smooth spiral channels in heat transfer and flow performance. With increasing corrugated number n, secondary flow intensity within the corrugated spiral channel intensifies. Notably, at a Reynolds number of 550, a multi-vortex structure emerges in the corrugated spiral channel with a corrugated number of 21. At Reynolds number 750 and corrugated number 21, the Nusselt number of corrugated spiral channel increases by 47.08% compared to the smooth spiral channel. At the same time, the resistance loss in the channel also increases, and the Fanning friction factor of the corrugated spiral channel increases by 59.20%. When the Reynolds number is 750, the PEC0 of the corrugated spiral channel with 18 corrugations is the highest, which increases by 27.66% compared to the smooth spiral channel. On this basis, longitudinal fins are added to the corrugated spiral channel, and it is observed by numerical simulation that symmetrical longitudinal vortices are induced by the fins. Further, the longitudinal fins induce symmetrical longitudinal vortices, enhancing flow and heat transfer in the channel's middle section. Under the condition of constant fin width and increasing fin height, the comprehensive heat transfer performance of the channel has a maximum value, that is, when fin width w=1/3W, fin height h=1/6H and Reynolds number Re=250, the highest PECw is 1.157. When the fin height is fixed, increasing the fin width w will also make the PECw of the channel first increase and then decrease. The correlation formulas for Nusselt number Nu, Fanning friction factor f and secondary flow intensity Se in corrugated spiral channel are fitted, with deviations within ±14.0%, ±10.2% and ±4.4%, respectively. This provides a certain reference for the application of corrugated spiral channels with longitudinal fins in the thermal design and usage of heat exchangers. [ABSTRACT FROM AUTHOR]

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Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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