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Traditionally, cellulose nanofiber (CNF) production has primarily relied on virgin cellulose sources. Yet, the shift to using paper mill sludge (PMS) as a source for CNF underscores the significance of reusing and recycling industrial byproducts. PMS contains significant amounts of cellulose that can be extracted as a raw material. The purpose of present study is to provide a sustainable approach to PMS utilization as a paper coating additive in the cellulose nanofibrils (CNFPMS) form via simply scalable wire-wound rod coating method. The effect of CNFPMS additive amounts at two coating layers on microstructure and surface properties of coatings such as porosity, air permeability surface roughness and optical properties such as brightness, gloss and CIE L*a*b* is studied, which they can also provide insight for the eventual print performance. Results indicated that the obtained CNFPMS in paper coating shows 52% decrease in porosity, presenting significant improvement in the coating microstructure. The marginal increase in permeability coefficient and surface roughness, 54% and 10%, respectively, suggests improving color reproduction and preventing color density losses. Optical analysis showed slight decrease in brightness and gloss, as was expected. Notably, the lightness was improved, which also indicates increasing color gamut volume in printing applications. As a result, the current work offers a sustainable approach to manage PMS for use in paper coatings as a high-value-added material. [ABSTRACT FROM AUTHOR]

The pulp and paper industry generates vast quantities of paper sludge, posing significant environmental challenges due to its disposal in landfills or incineration. This study explores the potential of valorizing paper sludge by incorporating it into particleboard production. It aims to optimize sludge content and particle size to enhance board properties—a novel approach to waste management in the wood composites industry. Through systematic variation of sludge content (0–25%) and particle size (< 0.5 to > 2 mm), we assessed the mechanical and physical properties such as internal bond strength (IB), modulus of rupture (MOR), modulus of elasticity (MOE), water absorption (WA), and thickness swelling (TS). The findings indicate that incorporating paper sludge at moderate levels (5–15%) with optimized particle sizes (< 1 mm) significantly improves the mechanical properties of the particleboard, including increased IB, MOR, and MOE while reducing WA and TS. Principal Component Analysis (PCA) further supported these results, revealing that higher-density boards with enhanced mechanical properties absorb less water, highlighting the interrelationship between structural integrity and moisture resistance. The PCA also identified thickness swelling as an independent factor, suggesting that while mechanical properties can be optimized, additional strategies are needed to control swelling. In conclusion, this study demonstrates that up to 15% paper sludge can be effectively used in particleboard production without compromising quality, provided particle size is carefully controlled. This approach not only offers a sustainable solution for managing paper sludge but also contributes to the development of eco-friendly composite materials, aligning with circular economy principles. [ABSTRACT FROM AUTHOR]

Paper and packaging materials a lignocellulose-based natural biodegradable polymer that spontaneously releases acetic acid, aldehydes, alcohol, and ester-based volatile organic compounds (VOCs) upon ageing and these VOCs start degrading the paper products and decline their mechanical strength properties. The reactivity of the paper of unbleached wheat straw pulp towards acetic acid and hexanal, which has been proven to have more degrading effects on paper than other VOCs, was considered in this work. The papers were exposed to these volatile compounds for 90 days in an air-tight vessel under ambient environmental conditions. The results showed that hexanal was more destructive than acetic acid with regards to cellulose degradation and depletion in the mechanical strength properties. The paper properties like, tensile, tear and burst index, viscosity, pH and carbonyl group content was measured. The growth of the carbonyl group, evidence of the ageing effects in the paper, detected more in the paper exposed to acetic acid. However, the strength of paper properties declined more with hexanal. FE-SEM analysis of the sample showed the development of pores and damage of cellulose fibre upon ageing. Similarly, the damaging effects of VOCs on cellulose, hemicelluloses and lignin were confirmed by significantly reduced peak detection through FT-IR \analysis. The high crystallinity index of the paper products due to exposure to VOCs was detected by XRD analysis, which confirmed the degradation of the low molecular weight cellulose molecule. Thus, the results are strongly recommended that VOCs that generates due to natural or artificial ageing could be the leading cause of paper degradation. [ABSTRACT FROM AUTHOR]

Trimethylsilyl cellulose (TMSC) was employed as the coating matrix for the application of zinc oxide nanoparticles (ZnO) onto paper surfaces and the protections of ZnO/TMSC coating against UV-induced damages and fungal spoilage were evaluated. Filter papers were immersed in 2% w/v TMSC solution loaded with ZnO and air-dried. Three ZnO/TMSC suspensions were prepared with 0.1, 0.5, and 1% w/v ZnO NPs. The presences of ZnO/TMSC protective layers were confirmed with ATR-IR spectroscopy. The coated papers exhibited high surface hydrophobicities. After the coated papers were subject to 365-nm UV irradiation at 400 W for 3 h, the contact angles dramatically dropped. The trimethylsilyl (TMS) groups exposed on the surface formed a moisture barrier and were partially removed on UV exposure. ATR-IR revealed that more TMS groups were removed in the protective layer with no ZnO. UV-irradiated papers turned yellow and papers protected with 1% ZnO/TMSC exhibited significantly lower color changes than that of the uncoated one. Compared to the TMSC-coated paper, the addition of ZnO resulted in a significant reduction in tensile strength at maximum. However, after UV irradiation, significant increases in both the strain at break and strength at maximum were only observed in 1% ZnO/TMSC-protected papers. Regarding their anti-fungal properties, the 1% ZnO/TMSC films were effective in growth inhibitions of Aspergillus sp. and Penicillium sp. on the nonirradiated papers. Despite being hydrophilic after UV-irradiation, growths of the molds were severely suppressed on the UV-irradiated paper. [ABSTRACT FROM AUTHOR]

Nucleic acid amplification testing has great potential for point-of-need diagnostic testing with high detection sensitivity and specificity. Current sample preparation is limited by a tedious workflow requiring multiple steps, reagents and instrumentation, hampering nucleic acid testing at point of need. In this study, we present the use of mixed cellulose ester (MCE) paper for DNA binding by ionic interaction under molecular crowding conditions and fluid transport by wicking. The poly(ethylene) glycol-based (PEG) reagent simultaneously provides the high pH for alkaline lysis and crowding effects for ionic binding of the DNA under high salt conditions. In this study, we introduce Paper-based Abridged Solid-Phase Extraction with Alkaline Poly(ethylene) Glycol Lysis (PASAP). The anionic mixed cellulose ester (MCE) paper is used as solid phase and allows for fluid transport by wicking, eliminating the need for pipetting skills and the use of a magnet to retain beads. Following the release of DNA from the cells due to the lytic activity of the PASAP solution, the DNA binds to the anionic surface of the MCE paper, concentrating at the bottom while the sample matrix is transported towards the top by wicking. The paper was washed by dipping it in 40% isopropanol for 10 s. After air-drying for 30 s, the bottom section of the paper (3 mm × 4 mm) was snapped off using the cap of a PCR tube and immersed in the colourimetric loop-mediated isothermal amplification (cLAMP) solution for direct amplification and colourimetric detection. The total sample processing was completed in 15 min and ready for amplification. cLAMP enabled the detection of 102 CFU/mL of Escherichia coli (E. coli) from culture media and the detection of E. coli in milk < 103 CFU/mL (10 CFU) after incubation at 68 °C for 60 min, demonstrating applicability of the method to complex biological samples. [ABSTRACT FROM AUTHOR]

In this paper, three kinds of micro-nano bamboo powder (MBP) and alkyl ketene dimer (AKD) were added to the polyvinyl alcohol/cellulose nanofiber (PVA/CNF) coating to prepare PVA/CNF/MBP coated paper and PVA/CNF/M-MBP/AKD coated paper. The results showed that MBP improved the oleophobicity of PVA/CNF coating, and the grease resistance grade of PVA/CNF/B-MBP and PVA/CNF/M-MBP coated papers reached the highest level, with a kit number of 12. Among the PVA/CNF/MBP coated papers, the PVA/CNF/M-MBP coated paper has the best hydrophobic properties, with the water contact angle and Cobb value of 74° and 21.3 g/m2, respectively. In addition, when the AKD dosage was 0.2% in the PVA/CNF/M-MBP/AKD coating, the kit number of the coated paper was 11, the Cobb value was 15.2 g/m2, the water contact angle was 103°, and the tensile strength was found to increase slightly. Therefore, compared with PVA/CNF coated paper, PVA/CNF/M-MBP/AKD coated paper has good strength and excellent hydrophobic and oleophobic properties. [ABSTRACT FROM AUTHOR]

Microfluidic paper-based analytical devices often are combined with scanners as detectors. In this work, different scanning options offered by scanners: resolution, scanning mode, exposure to radiation, colour restoration, and saving format were tested. Moreover, different attempts to mathematical data treatment based on intensities of three channels—Red, Green and Blue, were studied. All measurements presented in this article were conducted for a model dye—bromothymol blue and a model analyte—zinc(II) ion (complexed with xylenol orange in a paper matrix). The article summarizes the scanning options and possibilities of mathematical calculations. Nevertheless, it is suggested that the best option is to use the prior prepared calculation file to paste obtained intensities and compare all presented in this article (and the most frequently used) equations to process intensities and decide which one should be used in the particular analysis. [ABSTRACT FROM AUTHOR]

Anger suppression is important in our daily life, as its failure can sometimes lead to the breaking down of relationships in families. Thus, effective strategies to suppress or neutralise anger have been examined. This study shows that physical disposal of a piece of paper containing one's written thoughts on the cause of a provocative event neutralises anger, while holding the paper did not. In this study, participants wrote brief opinions about social problems and received a handwritten, insulting comment consisting of low evaluations about their composition from a confederate. Then, the participants wrote the cause and their thoughts about the provocative event. Half of the participants (disposal group) disposed of the paper in the trash can (Experiment 1) or in the shredder (Experiment 2), while the other half (retention group) kept it in a file on the desk. All the participants showed an increased subjective rating of anger after receiving the insulting feedback. However, the subjective anger for the disposal group decreased as low as the baseline period, while that of the retention group was still higher than that in the baseline period in both experiments. We propose this method as a powerful and simple way to eliminate anger. [ABSTRACT FROM AUTHOR]

The present study aimed to investigate indigenous bacteria possibility in recycled paper and cardboard mill (RPCM) wastewater treatment through the isolation and identification of full-scale RPCM indigenous bacteria. The molecular characterization of the isolated bacteria was performed by 16S rRNA gene sequencing. Klebsiella pneumoniae AT-1 (MZ599583), Citrobacter freundii AT-4 (OK178569), and Bacillus subtilis AT-5 (MZ323975) were dominant strains used for RPCM wastewater bioremediation experiments. Under optimal conditions, the maximum values of chemical oxygen demand (COD) and color biodegradation by C. freundii AT-4 were 79.54% and 43.81% after 10 days of incubation, respectively. In the case of B. subtilis strain AT-5 and K. pneumoniae AT-1, the maximum values of COD and color biodegradation were 70.08%, 45.96%, 71.26%, and 32.06%, respectively. The results from optimal conditions regarding efficiency were higher in comparison with the efficiency obtained from the oxidation ditch treatment unit in full-scale RPCM-WWTP. Therefore, the present study introduces the isolated indigenous bacteria strains as a promising candidate for improving the RPCM-WWTP efficiency using bioremediation. [ABSTRACT FROM AUTHOR]

In this work, we demonstrate an effective anion capturing in an aqueous medium using a highly porous carbon paper decorated with ZnO nanorods. A sol–gel technique was first employed to form a thin and compact seed layer of ZnO nanoparticles on the dense network of carbon fibers in the carbon paper. Subsequently, ZnO nanorods were successfully grown on the pre-seeded carbon papers using inexpensive chemical bath deposition. The prepared porous electrodes were electrochemically investigated for improved charge storage and stability under long-term operational conditions. The results show effective capacitive deionization with a maximum areal capacitance of 2 mF/cm2, an energy consumption of 50 kJ per mole of chlorine ions, and an excellent long-term stability of the fabricated C-ZnO electrodes. The experimental results are supported by COMSOL simulations. Besides the demonstrated capacitive desalination application, our results can directly be used to realize suitable electrodes for energy storage in supercapacitors. [ABSTRACT FROM AUTHOR]

The valorization of paper mill sludge (PMS) is the main goal of this study. The emissions of PMS continue to increase at global scale, especially from packaging paper and board sectors. The raw sludge was used to prepare an adsorbent to remove toxic pollutants from wastewater, the methylene blue (MB), an organic dye. Firstly, the physico-chemical characterization of PMS was done determining the crystalline phases of PMS fibers, the content of main elements, and the pH zero point charge, which was determined at around pH 7. The adsorption of MB on PMS powder was studied at 18 °C with an agitation of 200 rpm, being the best operating conditions 30 min of contact time, 250 mg L−1 of initial MB concentration and 0.05 g in 25 mL of adsorbent dose. Experimental data of MB adsorption was fitted to Langmuir and Freundlich isotherm equations. The Langmuir model was more accurate for the equilibrium data of MB adsorption at pH 5.1. The PFOM and PSOM were adjusted to experimental adsorption kinetics data, being PSOM, which describes better the MB adsorption by PMS powder. This was confirmed by calculating the maximum adsorption capacity with PSOM, which was 42.7 mg g−1, being nearly similar of the experimental value of 43.5 mg g−1. The analysis of adsorption thermodynamics showed that the MB was adsorbed exothermically with a ΔH0 = − 20.78 kJ mol−1, and spontaneously with ΔG0 from − 0.99 to − 6.38 kJ mol−1 in the range of temperature from 291 to 363 K, respectively. These results confirm that the sludge from paper industry can be used as biosorbent with remarkable adsorption capacity and low cost for the treatment of wastewater. PMS can be applied in the future for the depollution of the effluents from the textile industry, which are highly charged with dyes. [ABSTRACT FROM AUTHOR]

Energy storage devices (ESD) which are intended to power electronic devices, used in close contact of human skin, are desirable to be safe and non-toxic. In light of this requirement, Zn based energy storage devices seem to provide a viable pathway as they mostly employ aqueous based electrolytes which are safe and non-toxic in their functioning. Additionally, having a flexible ESD will play a crucial role as it will enable the ESD to conform to the varying shapes and sizes of wearable electronics which they energize. In this work, we have developed an inkjet-printed Zinc ion battery (IPZIB) with planar electrode configuration over bond paper substrate. Zn has been used as the negative electrode, MnO2 is used as the positive electrode with Poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) as the active binder. Conducting tracks of reduced graphene oxide (rGO) are used to construct the current collector on the paper substrate. The fabricated IPZIB delivered a high discharge capacity of 300.14 mAh g−1 at a current density of 200 mA g−1. The energy density of the IPZIB is observed as 330.15 Wh kg−1 at a power density of 220 W kg−1 and retains an energy density of 94.36 Wh kg−1 at a high power density of 1650 W kg−1. Finally, we have demonstrated the capability of the IPZIB to power a LED at various bending and folding conditions which indicates its potential to be used in the next generation flexible and wearable electronic devices. [ABSTRACT FROM AUTHOR]

Laserjet printing is a kind of facile and digital do-it-yourself strategies, which is of importance to fabricate inexpensive paper-based microfluidic devices. However, the printed hydrophobic barrier is not hydrophobic enough due to the weak hydrophobicity and requires subsequent heating, which can lead to the pyrolysis of cellulose in the paper and influence the detection results. Here, for the first time, we report a kind of functional toner including toner and polydopamine (PDA) nanocapsules which contains oleic acid modified ferric tetroxide (OA-Fe3O4) and octadecylamine (ODA), which is suitable for printing with desired shapes and sizes to lead to formation of superhydrophobic barriers. Moreover, patterns printed with functional toner have good stability, including resistance to moisture, ultraviolet (UV) and bending. Finally, a proof-of-concept of metal and nitrite ions testing is demonstrated using colorimetric analysis, and the results show that the printed devices successfully perform instant detection of ions. The developed functional toner offers easy fabrication, cost-effectiveness and mass production of paper-based devices. In general, this strategy provides a new idea and technical support for the rapid prototyping of microfluidic paper-based analytical devices (μPADs) using laserjet printing. [ABSTRACT FROM AUTHOR]

Bloodstream infection (BSI) is a major complication in patients with cancers due to therapy-induced neutropenia and underlying conditions, which increases hospitalization time and mortality rate. Targeted and timely antimicrobial management is crucial to save the patients' lives and reduce the social and economic burdens. Blood culture is a routine clinical diagnostic method of BSI with a long turnaround time, and generally identifies monomicrobial BSI. Thus, polymicrobial BSI often goes undetected although it occurs more frequently in these patients and results in more severe outcomes compared to monomicrobial BSI. In this work, we apply glutaric anhydride, N-hydroxysuccinimide and N,N′-dicyclohexylcarbodiimide to fabricate a functional surface on cellulose filter paper. Targeting three pathogens (Escherichia coli, Saccharomyces cerevisiae, and human cytomegalovirus) commonly occurring in BSI in neutropenic patients, we demonstrate rapid and accurate triplex pathogen DNA detection using the functionalized paper. All three pathogen DNA was identified in 1–5 min with a detection limit of 0.1–0.5 ng/µL. The developed test tool has the potential to provide rapid polymicrobial BSI diagnosis in support of timely, accurate antimicrobial treatment, and could be integrated into an automatic sample-to-result portable equipment. [ABSTRACT FROM AUTHOR]

As the social organism, Polistes wasps build a communal nest using woody fibers with saliva for sustaining brood and adult population throughout the season. Limited information exists regarding the identification specific plant materials employed in wasp nest building. Thus, we firstly tested if the DNA metabarcoding approach utilizing rbcL and trnL molecular markers could identify the plant species quantitatively and qualitatively inform the mixed-origin woody samples. A threshold of 0.01 proportion of reads was applied for rbcL and trnL molecular markers, while this threshold for median proportion was 0.0025. In assessing taxa richness, the median proportion demonstrated superior performance, exhibiting higher taxa detection power, however, rbcL marker outperformed in quantitative analysis. Subsequently, we applied DNA metabarcoding to identify the plant materials from the nests of two Polistes species, P. mandarinus and P. rothneyi. The results showed that higher preference of Quercus and Robinia as the major nest building materials regardless of the surrounding plant communities, by two wasp species. Material diversity was higher for P. rothneyi than P. mandarinus, which may explain the abundance of this species possibly with heightened adaptive capacities in their nesting behavior. This study demonstrated that DNA metabarcoding could identify the complex nest-building plant materials of paper wasps and provide insights into their ecological interactions in the natural ecosystem. [ABSTRACT FROM AUTHOR]

The wettability of the paper surface is greatly affected by the wettability of the pulp fibers. We conducted this study in order to understand the relationship between the wettability of a single fiber of recycled pulp and the strength of recycled paper, as well as the inter-fiber bonding strength. The contact angle was determined from a series of photographs of the pulp fiber and the water silhouettes at the point of contact. The contact line and profile history were continuously photographed in every 1 s after the initial contact. The recycled softwood kraft pulp fibers were clearly much less hydrophilic than the original fibers, regardless of whether the fibers had been bleached or not. The contact angle of the original chemi-thermomechanical pulp fiber was much higher than that of the original softwood bleached kraft pulp fiber. Furthermore, increased number of recycling decreased the contact angle of the chemi-thermomechanical pulp fiber. The Page equation was used to evaluate the strength contributions of single fiber and fiber–fiber bonding to tensile strength of paper. As a result, an increase in weakness factor of fiber–fiber bonding strength was obtained for the recycled softwood kraft pulp handsheet. On the other hand, the weakness factor of the original chemi-thermomechanical pulp handsheet decreased with recycling. In addition, the weakness factor of fiber–fiber bonding strength and the contact angles of the provided softwood bleached kraft pulp fibers bore a proportional relationship to each other. [ABSTRACT FROM AUTHOR]

Rapid and accurate bioburden detection has become increasingly necessary for food, health, pharmaceutical and environmental applications. To detect bioburden accurately, and in a highly sensitive manner, we have fabricated a novel microfluidic device with an integrated filter to trap the cells. Bioburden is detected on the filter paper in situ using the redox reaction of fluorescent label resorufin and a portable multichannel fluorometer is used for fluorescence measurement. The microfluidic device was fabricated in a facile, low-cost, and rapid way with microwave-induced thermally assisted bonding. To characterize the bonding quality of the microfluidic cassettes, different tests were performed, and the filter paper material and size were optimized. Primary Bacillus subtilis culture bacterial samples were filtered through the device to validate and investigate the performance parameters. Our results show that a limit of detection (LOD) of 0.037 CFU/mL can be achieved through this microfluidic device whereas the LOD in a normal microfluidic cassette in the fluorometer and the golden standard spectrophotometer are 0.378 and 0.128 CFU/mL respectively. The results depict that three to ten times LOD improvement is possible through this microfluidic cassette and more sensitive detection is possible depending on the volume filtered within a rapid 3 min. This novel microfluidic device along with the fluorometer can be used as a rapid portable tool for highly sensitive, accurate and high-throughput bacterial detection for different applications. [ABSTRACT FROM AUTHOR]

Paper coating plays an important role in the paper properties, printability and application. The nanocoating is a multifunction layer that provides the paper with unique features. In this work, nanocoating formulas were prepared using a green method and component. The nanocoating formulas were based on biopolymers nanostarch NSt and nanochitosan NCh (NCS) decorated with Egyptian kaolinite Ka doped with zinc nanoparticles NCS@xka/ZnONPs (x represents different ratios) support for multifunctional uses. The nanocoating formulas were characterized using a physiochemical analysis as well as a topographical study. FTIR, XRD, SEM and TEM techniques were used. Additionally, the antimicrobial activity of the tested samples was assessed against six microorganisms including Gram-negative and Gram-positive bacteria. The prepared nanocoating formulas affirmed excellent antimicrobial activity as a broad-spectrum antimicrobial active agent with excellent activity against all representative microbial communities. The nanocoating with the highest ratio of Ka/ZnONPs (NCS@40 ka/ZnONPs) showed excellent antimicrobial activity with an inhibition percentage of more than 70% versus all microorganisms presented. The paper was coated with the prepared suspensions and characterized concerning optical, mechanical and physical properties. When Ka/ZnONPs were loaded into NCS in a variety of ratios, the characteristics of coated paper were enhanced compared to blank paper. The sample NCS@40 ka/ZnONPs increased tensile strength by 11%, reduced light scattering by 12%, and improved brightness and whiteness by 1%. Paper coated with NCh suspension had 35.32% less roughness and 188.6% less porosity. When coated with the sample NCS@10 ka/ZnONPs, the coated paper's porosity was reduced by 94% and its roughness was reduced by 10.85%. The greatest reduction in water absorptivity was attained by coating with the same sample, with a reduction percentage of 132%. [ABSTRACT FROM AUTHOR]

Cellulose fibers are a major industrial input, but due to their irregular shape and anisotropic material response, accurate material characterization is difficult. Single fiber tensile testing is the most popular way to estimate the material properties of individual fibers. However, such tests can only be performed along the axis of the fiber and are associated with problems of enforcing restraints. Alternative indirect approaches, such as micro-mechanical modeling, can help but yield results that are not fully decoupled from the model assumptions. Here, we compare these methods with nanoindentation as a method to extract elastic material constants of the individual fibers. We show that both the longitudinal and the transverse elastic modulus can be determined, additionally enabling the measurement of fiber properties in-situ inside a sheet of paper such that the entire industrial process history is captured. The obtained longitudinal modulus is comparable to traditional methods for larger indents but with a strongly increased scatter as the size of the indentation is decreased further. [ABSTRACT FROM AUTHOR]

There is increasing focus on applying deep learning methods to electrocardiograms (ECGs), with recent studies showing that neural networks (NNs) can predict future heart failure or atrial fibrillation from the ECG alone. However, large numbers of ECGs are needed to train NNs, and many ECGs are currently only in paper format, which are not suitable for NN training. We developed a fully-automated online ECG digitisation tool to convert scanned paper ECGs into digital signals. Using automated horizontal and vertical anchor point detection, the algorithm automatically segments the ECG image into separate images for the 12 leads and a dynamical morphological algorithm is then applied to extract the signal of interest. We then validated the performance of the algorithm on 515 digital ECGs, of which 45 were printed, scanned and redigitised. The automated digitisation tool achieved 99.0% correlation between the digitised signals and the ground truth ECG (n = 515 standard 3-by-4 ECGs) after excluding ECGs with overlap of lead signals. Without exclusion, the performance of average correlation was from 90 to 97% across the leads on all 3-by-4 ECGs. There was a 97% correlation for 12-by-1 and 3-by-1 ECG formats after excluding ECGs with overlap of lead signals. Without exclusion, the average correlation of some leads in 12-by-1 ECGs was 60–70% and the average correlation of 3-by-1 ECGs achieved 80–90%. ECGs that were printed, scanned, and redigitised, our tool achieved 96% correlation with the original signals. We have developed and validated a fully-automated, user-friendly, online ECG digitisation tool. Unlike other available tools, this does not require any manual segmentation of ECG signals. Our tool can facilitate the rapid and automated digitisation of large repositories of paper ECGs to allow them to be used for deep learning projects. [ABSTRACT FROM AUTHOR]

Uropathogenic Escherichia coli (UPEC) causes up to 90% of urinary tract infections (UTI) which is more prevalent among females than males. In urine, patients with symptomatic UTI usually have a high concentration of bacterial infection, ≥ 105 colony-forming units (CFU) per mL, in which the culture method is regularly the gold standard diagnosis. In this study, a simple and inexpensive distance-based paper device (dPAD) combined with the fluorescent closed tube LAMP assay was validated for simultaneously screening and semi-quantifying the infection level of E. coli in 440 urine samples of patients with UTI. The dPAD could measure the LAMP amplicons and semi-quantify the levels of E. coli infection in heavy (≥ 104 CFU/mL), light (≤ 103 CFU/mL) and no infection. The sensitivity and specificity had reliable performances, achieving as high as 100 and 92.7%, respectively. The one step LAMP assay could be performed within 3 h, which was 7.5 times faster than the culture method. To empower early UTI diagnosis and fast treatment, this inexpensive dPAD tool combined with the fluorescent closed tube LAMP assay is simple, reliably fast and practically portable for point-of-care settings, particularly in resource-limited areas, which can be set up in all levels of healthcare facilities. [ABSTRACT FROM AUTHOR]

In this work heat transfer papers were loaded with a new core–shell pigment based on precipitating thin shell of titanium dioxide on a core of rice husk silica ash TiO2/RHSA to be applied in dye sublimation printing of textile fabrics. Besides, 0.1% (w/w) cationic polyacrylamide (CPAM) and 1% (w/w) bentonite (Bt) were also added sequentially to improve drainage and filler retention of the paper hand-sheets made from bleached kraft bagasse pulps. The effect of the new core–shell pigment on the mechanical and barrier properties, thermal stability and surface morphology of modified paper sheets were investigated. In addition, the study of transfer printability and ease of dye release from paper to fabric in this heat transfer printing of polyester fabrics using silk-screen printing under different transfer parameters were studied. Also, fastness measurements including washing, light and perspiration of printing polyester fabric were also estimated. [ABSTRACT FROM AUTHOR]

To achieve integrated resource utilization of graphite tailings to improve their water-holding capacity, river silt and cow dung powder were added to graphite tailings as organic matter improvers. Improver ratios were designed using 4 g cow dung powder and 20, 30, and 50 g river silt. Soil–water characterization tests were performed using a combined tensiometer and filter paper method based on optimum density measurements. Analysis of the influence of river silt dosing on the soil–water characteristic curves of improved graphite tailing specimens was performed with data fitting using the Van Genuchten model. Here, we investigated the effect of river silt dosing on the internal pore structure and water-holding capacity of modified graphite tailing samples and verified the applicability of the model to graphite tailings. Our results demonstrate that the organic matter improver incorporated into graphite tailings can improve the internal structural compactness of graphite tailings, improving the water holding capacity. With an increase in river silt dosage, the saturated water content is larger, and the residual water content increases and then decreases. When river silt dosage is 30 g, the residual water content is the highest at a value of 3.32%. The van Genuchten model was highly accurate for assessing the graphite tailings. With an increase in river silt doping, the internal pore space first decreased and then increased, and the internal structure gradually became compact and loosened. The internal structure was in the optimal state in the experimental study when the dosage of cow dung powder was 4 g and the dosage of river silt was 30 g. The water holding capacity was optimal at this time. The results of this study provide a theoretical foundation for graphite-tailing-based mine reclamation and play a guiding role in exploring the value of the hydraulic characteristic index parameters when applying graphite tailings engineering. [ABSTRACT FROM AUTHOR]

Metagenomics has revolutionized access to genomic information of microorganisms inhabiting the gut of herbivorous animals, circumventing the need for their isolation and cultivation. Exploring these microorganisms for novel hydrolytic enzymes becomes unattainable without utilizing metagenome sequencing. In this study, we harnessed a suite of bioinformatic analyses to discover a novel cellulase-degrading enzyme from the camel rumen metagenome. Among the protein-coding sequences containing cellulase-encoding domains, we identified and subsequently cloned and purified a promising candidate cellulase enzyme, Celcm05-2, to a state of homogeneity. The enzyme belonged to GH5 subfamily 4 and exhibited robust enzymatic activity under acidic pH conditions. It maintained hydrolytic activity under various environmental conditions, including the presence of metal ions, non-ionic surfactant Triton X-100, organic solvents, and varying temperatures. With an optimal temperature of 40 °C, Celcm05-2 showcased remarkable efficiency when deployed on crystalline cellulose (> 3.6 IU/mL), specifically Avicel, thereby positioning it as an attractive candidate for a myriad of biotechnological applications spanning biofuel production, paper and pulp processing, and textile manufacturing. Efficient biodegradation of waste paper pulp residues and the evidence of biopolishing suggested that Celcm05-2 can be used in the bioprocessing of cellulosic craft fabrics in the textile industry. Our findings suggest that the camel rumen microbiome can be mined for novel cellulase enzymes that can find potential applications across diverse biotechnological processes. [ABSTRACT FROM AUTHOR]

For shaped charge blasting projects in mining, civil engineering, and similar fields, it is proposed to modify the charge structure by combining slotted tubes and shaped charge liners to obtain a new type of charge structure. This aims to achieve directional rock breaking through the focused action of the shaped charge. The influence of different slotted pipe materials on the directional rock-breaking effect of concentrated energy using a new charge structure is explored through theoretical analysis combined with model test study, high-speed camera, stress–strain gauge, and other equipment. A comparison is made between slotted pipes made of aluminum, kraft paper, and PVC, with the cutting width of 2 mm. Based on the characteristics of the cracks formed after blasting, the new charge structure made of aluminum slotted pipe produces a penetrating crack that is almost consistent with the pre-cracking direction. Based on the corresponding characteristics of successively released blasting energy, the guiding and convergence effect of the new charge structure made of aluminum slotted pipe on the explosion energy is greater than that of the new charge structure made of the other two types of slotted pipe material. According to the strain data measured after blasting, the peak arrival time of the strain peak in the direction of the slotted pipe on one side of the shaped hood is shorter than that in the other two directions, and the peak strain is greater than that in the other two directions while having a better energy gathering effect. Based on the findings, the new charge structure with directional energy concentration has a damage reduction effect. Furthermore, the material of aluminum slotted pipe is found to be better than PVC slotted pipe, whereas the material of PVC slotted pipe is better than kraft paper slotted pipe in achieving directional rock breaking. [ABSTRACT FROM AUTHOR]

A one-step analysis method was developed for four types of amino acids using a microfluidic paper-based analytical device fabricated from chromatography filtration paper and laminate films. Aminoacyl-tRNA synthetase was used to detect each amino acid. The obtained laminated paper-based analytical device (LPAD) contained four enzymatic reaction areas. Colorimetric detection was performed based on the molybdenum blue reaction. A model method for the simple, easy, and simultaneous detection of several amino acid concentrations was suggested, in contrast to the conventional methods such as HPLC or LC–MS. The method provided a selective quantification at the ranges of 3.6–100 μM for tryptophan, 10.1–100 μM for glycine, 5.9–100 μM for histidine and 5.6–100 μM for lysine with a detection limit of 1.1 μM, 3.3 μM, 1.9 μM and 1.8 μM, respectively. LPAD fabrication was considerably simple, and the subsequent detection process was easy and required a short period of time (within 15 min). [ABSTRACT FROM AUTHOR]

Polymers are often combined with magnetostrictive materials to enhance their toughness. This study reports a cellulose nanofibril (CNF)-based composite paper containing dispersed CoFe2O4 particles (CNF–CoFe2O4). Besides imparting magnetization and magnetostriction, the incorporation of CoFe2O4 particles decreased the ultimate tensile strength and increased the fracture elongation of the CNF–CoFe2O4 composite paper. CNF was responsible for the tensile properties of CNF–CoFe2O4 composite paper. Consequently, the magnetic and magnetostrictive properties and tensile properties of CNF–CoFe2O4 composite paper can be controlled by changing the mixture ratio of CNF and CoFe2O4 particles. [ABSTRACT FROM AUTHOR]

In the present work, the composite cross-linked were used to consolidate the dyed paper manuscripts. Nanocomposites of mesoporous silica nanoparticle (MPSNP)/polyvinyl alcohol (PVA) and cellulose nanofiber (CNF)/PVA, which have never been used before, have been evaluated for the consolidation process of the dyed paper manuscripts with madder extract. Three concentrations 1%, 3%, and 5% have been prepared. Analysis and investigation methods like scanning electron microscope (SEM), transmission electron microscope (TEM), dynamic light scattering analysis (DLS), X-Ray diffraction Analysis (XRD), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR) and total color difference (ΔE) by spectrophotometer have been used in order to characterize the prepared nano-sized composites and evaluate the treated dyed paper samples before and after the aging process. The results of surface morphology by SEM revealed the effectiveness of MPSNP/PVA core–shell nanocomposite at 5% in the consolidation process, where the improvement of properties of the aged dyed paper samples. The fibers of the treated paper became strong and appeared clearly. The result of ΔE measurements showed that the treated sample with MPSNP/PVA nanocomposite at 5% gave the lowest ΔE (5.22), while, the treated sample with CNF/PVA nanocomposite at 5% gave the highest ΔE value (11.66). Mechanical measurements (tensile strength and elongation) revealed the efficiency of MPSNP/PVA nanocomposite at 5% in the treatment of the aged dyed paper samples. The treated sample with the mentioned material gave tensile strength and elongation values of 84.8 N/nm2 and 1.736%, respectively. In contrast, the treated sample with CNF/PVA nanocomposite at 1% gave the lowest tensile strength and elongation values 38.2 N/nm2, and 1.166%, respectively. FTIR analysis revealed an increase was noticed in the CH2 stretching band (refers to the crystallinity of cellulose), where the intensity of the treated sample with MPSNP/PVA nanocomposite was at a 5% increase compared to the control sample. The FTIR results supported the results of mechanical measurements. The intensity of the CH2 stretching band, which refers to the crystallinity index of cellulose, was increased with the use of MPSNP/PVA nanocomposite at 3% and 5%, which explains the improvement in mechanical properties. This may be due to the nano-mineral particles, which improve the mechanical properties. Additionally, they reduce the effect of accelerated thermal aging on the cellulosic fibers and give them stability. The detailed analysis of analytical methods used for evaluation revealed the novelty of MPSNP/PVA nanocomposite, especially at 5%. It has a potential role in strengthening and improving different properties of the dyed paper manuscripts with madder extract. [ABSTRACT FROM AUTHOR]

While traditional techniques have long allowed forensic investigators to positively identify fingermarks on documents of interest, understanding the chronological sequence of events that led to their deposition is still seen as a 'holy grail' for forensic examinations. By way of example, the question of whether a mark is above or below printed text is crucial. The work herein reveals that a novel application of a recently established fingermark development technique readily allows such differentiation. The process in question allies forensic gelatin lifters with RECOVER, a development system that hinges on the polymerisation of disulfur dinitride. While the latter was specifically developed in its current form for the retrieval of prints from metal surfaces exposed to extreme conditions or washing, its ability to target surface effects allows for visualisation of surface interactions on forensic gelatin lifts. Crucially, in doing so the order in which the lifted material was originally deposited is also revealed. This, therefore, permits clear elucidation of the order of deposition of printed text and fingermarks—and does so both rapidly and in a non-invasive way. This long sought-after capability has the potential to revolutionise forensic document examinations. [ABSTRACT FROM AUTHOR]

We aimed to investigate differences in patients' anxiety and satisfaction between patients undergoing paper-based patient decision aid (PDA) for shared decision-making (SDM) and those receiving computer-based PDA. We retrospectively collected questionnaires before and after SDM. Basic demographic data as well as anxiety, satisfaction, knowledge acquisition, and participation in SDM were recorded. We divided our population into subgroups according to use of paper-based or computer-based PDA. In addition, Pearson correlation analysis was applied to assess the relationships among variables. In total, 304 patients who visited our Division of Nephrology were included in the final analysis. Overall, over half of the patients felt anxiety (n = 217, 71.4%). Near half of the patients felt a reduction in anxiety after SDM (n = 143, 47.0%) and 281 patients (92.4%) were satisfied with the whole process of SDM. When we divided all the patients based on use of paper-based or computer-based PDA, the reduction of anxiety level was greater in the patients who underwent paper-based PDA when compared with that of those who underwent computer-based PDA. However, there was no significant difference in satisfaction between the two groups. Paper-based PDA was as effective as computer-based PDA. Further studies comparing different types of PDA are warranted to fill the knowledge gaps in the literature. [ABSTRACT FROM AUTHOR]

Although often regarded a childhood toy, the design of paper airplanes is subtly complex. The design space and mapping from geometry to distance flown is highly nonlinear and probabilistic where a single airplane design exhibits a multitude of trajectory forms and flight distances. This makes optimization and understanding of their behavior challenging for humans. By understanding the behavior of paper airplanes and predicting flight behavior, there is a potential to improve the design of aerial vehicles that operate at low Reynolds numbers. By developing a robotic system that can fabricate, test, analyze, and model the flight behavior in an unsupervised fashion, a wide design space can be reliably characterized. We find there are discrete behavioral groups that result in different trajectories: nose dive, glide, and recovery glide. Informed by this characterization we propose a method of using Gaussian mixture models to extract the clusters of the design space that map to these different behaviors. This allows us to solve both the forward and reverse design problem for paper airplanes, and also to perform efficient optimization of the geometry for a given target flight distance. [ABSTRACT FROM AUTHOR]

This study introduces a novel geotechnical composite material comprising two types of fill material sourced from the paper industry—deinking paper sludge ash (DPSA) and deinking paper sludge (DPS). Five composites with different DPSA and DPS contents were investigated. Two composites were selected for further analyses. The technology and procedure for composite installation were implemented in field tests. The composites with 80% and 70% DPSA exhibited the elasticity required to withstand minor landslide slip deformations, in addition to achieving sufficiently high values of uniaxial compressive strength. The composites had a low maximum dry density value, which led to fewer settlements in the entire support structure. The enhanced shear characteristics can enable the construction of a thinner retaining wall. The delay between preparation and installation of the composites was further investigated. The field tests confirmed that the composites with 80% and 70% DPSA can be installed on the construction site 4 h and even 24 h after mixing. In 2018, a retaining wall structure with 70% DPSA and 30% DPS was successfully implemented near a railway line using conventional technology as followed-up research to the herein presented study. Results have been derived from work performed in the scope of the H2020 Paperchain project in which novel circular economy models centered on the valorization of the waste streams generated by the pulp and paper industry as secondary raw material for several resource-intensive sectors, including the construction sector, have been developed. Environmental benefits are savings in natural raw materials, reduction of landfill disposal as well as CO2 emission reduction. [ABSTRACT FROM AUTHOR]

The properties of paper products depend on the structure of the cellulose fibres therein. Although fibre properties in virgin pulps can be modified by a refining process, this is more difficult in pulp from recovered fibre, particularly waste from office shredders that tend to shorten fibres during shredding. The shorter fibres in shredded paper make it difficult to easily reconstitute them into high-quality paper products. Moreover, because of high energy usage during the recycling process and transportation inefficiencies, there is a need to determine how to responsibly shred paper to alleviate this environmental burden. With this in mind, the influence of initial fibre length on the tensile properties of paper was investigated. Changes in initial fibre length significantly influenced many pulp and paper properties. It was found that cutting the paper into pieces with an area less than 25 mm2 caused significant changes in the important morphological parameters of the fibres and a sharp decrease in the tensile properties of the reconstituted paper. [ABSTRACT FROM AUTHOR]

This study performs a chemical investigation of blood plasma samples from patients with and without fibromyalgia, combined with some of the symptoms and their levels of intensity used in the diagnosis of this disease. The symptoms evaluated were: visual analogue pain scale (VAS); fibromyalgia impact questionnaire (FIQ); Hamilton anxiety rating scale (HAM); Tampa Scale for Kinesiophobia (TAMPA); quality of life Questionnaire—physical and mental health (QL); and Pain Catastrophizing Scale (CAT). Plasma samples were analyzed by paper spray ionization mass spectrometry (PSI-MS). Spectral data were organized into datasets and related to each of the symptoms measured. The datasets were submitted to multivariate classification using supervised models such as principal component analysis with linear discriminant analysis (PCA-LDA), successive projections algorithm with linear discriminant analysis (SPA-LDA), genetic algorithm with linear discriminant analysis (GA-LDA) and their versions with quadratic discriminant analysis (PCA/SPA/GA-QDA) and support vector machines (PCA/SPA/GA-SVM). These algorithm combinations were performed aiming the best class separation. Good discrimination between the controls and fibromyalgia samples were observed using PCA-LDA, where the spectral data associated with the CAT symptom achieved 100% classification sensitivity, and associated with the VAS symptom achieved 100% classification specificity, with both symptoms at the moderate level of intensity. The spectral variable at 579 m/z was found to be substantially significant for classification according to the PCA loadings. According to the human metabolites database, this variable can be associated with a LysoPC compound, which comprises a class of metabolites already evidenced in other studies for fibromyalgia diagnosis. This study proposed an investigation of spectral data combined with clinical data to compare the classification ability of different datasets. The good classification results obtained confirm this technique is as a good analytical tool for the detection of fibromyalgia, and provides theoretical support for other studies about fibromyalgia diagnosis. [ABSTRACT FROM AUTHOR]

In medical institutions, radiation shielding is an effective strategy to protect medical personnel and patients from exposure. Reducing the weight of the shield worn by medical personnel in the radiation generating area plays a key role in improving their productivity and mobility. In this study, a new lightweight radiation shield was developed by electrospinning a polymer-tungsten composite material to produce nanofibers with a multi-layered thin-film structure similar to that of a morpho butterfly wing. The fabricated shield was in the form of 0.1 mm thick flexible shielding paper. The multi-layer structure of the thin shielding paper was obtained through nanofiber pattern formation via electrospinning a dispersion of tungsten particles. At 0.1 mm thickness, the paper's shielding rate was 64.88% at 60 keV. Furthermore, at 0.3 mm thick and arranged in a laminated structure, the shielding rate was 90.10% and the lead equivalent was 0.296 mmPb. When used as an apron material, the weight can be reduced by 45% compared to existing lead products. In addition, the material is highly processable and can be used to manufacture various flexible products, such as hats, gloves, underwear, and scarves used in medical institutions. [ABSTRACT FROM AUTHOR]

The study's goal was to develop a spore-based paper strip biosensor for detecting ß-lactam antibiotics in milk using the enzyme induction principle. A new spore-based paper strip biosensor has been developed after important operating parameters such as spore volume, substrate volume, exposure time and temperature, and incubation time and temperature were optimised. The limit of detection for various ß-lactam antibiotics, including amoxicillin, penicillin, ampicillin, carbenicillin, cloxacillin, nafcillin, oxacillin, cephalothin, cefalexin, cefoxitin, cefazolin, and cefuroxime, was determined in milk with detection sensitivity of 1 ppb, 2 ppb, 2 ppb, 10 ppb, 10 ppb, 10 ppb, 20 ppb, 10 ppb 1000 ppb, 10 ppb 300 ppb and 100 ppb, respectively. It was also tested with other contaminants such non-ß-lactam antibiotics, pesticides, aflatoxin, heavy metals, and other chemical contaminants, and no interference was found, indicating that the created biosensor had a low rate of false positive and negative results. In comparison to the AOAC-approved CHARM-ROSA ß-lactam strip test, which identified 7 raw milk and zero pasteurised milk samples positive for ß-lactam antibiotics, the sensor was further analysed and verified using 200 raw milk and 105 pasteurised milk samples. This indicates a perfect match between our biosensor and the AOAC-approved CHARM-ROSA ß-lactam strip test. The developed spore-based paper strip biosensors are expected to be useful in the rapid and cost-effective detection of ß-lactam antibiotic residues in milk samples at the dairy farm, reception dock, and production units, respectively. [ABSTRACT FROM AUTHOR]

Non-Hermitian topology is a recent hot topic in condensed matters. In this paper, we propose a novel platform drawing interdisciplinary attention: rock–paper–scissors (RPS) cycles described by the evolutionary game theory. Specifically, we demonstrate the emergence of an exceptional point and a skin effect by analyzing topological properties of their payoff matrix. Furthermore, we discover striking dynamical properties in an RPS chain: the directive propagation of the population density in the bulk and the enhancement of the population density only around the right edge. Our results open new avenues of the non-Hermitian topology and the evolutionary game theory. [ABSTRACT FROM AUTHOR]

The simplest point-of-care assays are usually paper and plastic devices that detect proteins or nucleic acids at low cost and minimal user steps, albeit with poor limits of detection. Digital assays improve limits of detection and analyte quantification by splitting a sample across many wells (or droplets), preventing diffusion, and performing analyte amplification and detection in multiple small wells. However, truly digital nucleic acid amplification tests (NAATs) require costly consumable cartridges that are precisely manufactured, aligned, and operated to enable low detection limits. In this study, we demonstrate how to implement near-digital NAATs in low-cost porous media while approaching the low limits of detection of digital assays. The near-digital NAAT was enabled by a paper membrane containing lyophilized amplification reagents that automatically, passively meters and distributes a sample over a wide area. Performing a NAAT in the paper membrane while allowing diffusion captures many of the benefits of digital NAATs if the pad is imaged at a high spatial resolution during amplification. We show that the near-digital NAAT is compatible with a low-cost paper and plastic disposable cartridge coupled to a 2-layer rigid printed circuit board heater (the MD NAAT platform). We also demonstrate compatibility with biplexing and imaging with mobile phones with different camera sensors. We show that the near-digital NAAT increased signal-to-noise ratios by ~ 10×, improved limits of detection from above 103 copies of methicillin-resistant Staphylococcus aureus genomic DNA to between 100 and 316 copies in a biplexed reaction containing 105 copies of co-amplifying internal amplification control DNA, and reduced time-to-result from 45 min of amplification to 15–20 min for the positive samples. [ABSTRACT FROM AUTHOR]

Accurate quantification of blood creatinine is important to estimate the glomerular filtration rate. Existing techniques using liquid chromatography tandem mass spectrometry (LC–MS/MS) have a high accuracy and eliminate most interferences encountered in routine enzymatic and Jaffé methods. However, they require laborious and time-consuming sample treatment and data acquisition. The aim of this study is to develop a fast and simple method to enable a direct analysis of whole blood creatinine with performance measures that are comparable to conventional LC–MS/MS. 5μL whole blood is formed as a three-dimensional spheroid on hydrophobic silanized paper substrates which then undergoes paper-spray ionization—tandem mass spectrometry (PSI–MS/MS). The method is validated using real human samples and compared with LC–MS/MS. PSI–MS/MS whole blood analysis exhibited a lower limit of quantification of 2.5 μg/mL, precision ≤ 6.3%, recovery in the range of 88–94% and excellent linearity (R2 > 0.99; 2.5—20 μg/mL) covering the normal range for creatinine levels. Creatinine levels were comparable to those measured by LC–MS/MS with small deviations of less than 0.3 μg/mL. This simple, fast and accurate microsampling technique for direct analysis of creatinine from whole blood shows promise for routine clinical screening and monitoring. This approach can be readily extended for other analytes of interest and, due to inherent advantages relating to cost, storability, speed, and simplicity, it can be especially advantageous for use in resource-limited settings. [ABSTRACT FROM AUTHOR]

Various agents, including ethylenediaminetetraacetic acid, oxalic acid, citric acid, and HCl, were applied to remove heavy metals from raw paper incineration ash and render the ash recyclable. Among these prepared agent solutions, ethylenediaminetetraacetic acid showed the highest efficiency for Pb removal, while oxalic acid showed the highest efficiencies for Cu, Cd, and As removal. Additionally, three modes of an advanced removal method, which involved the use of both ethylenediaminetetraacetic acid and oxalic acid, were considered for use at the end of the rendering process. Among these three modes of the advanced removal method, that which involved the simultaneous use of ethylenediaminetetraacetic acid and oxalic acid, i.e., a mixture of both solutions, showed the best heavy metal removal efficiencies. In detail, 11.9% of Cd, 10% of Hg, 28.42% of As, 31.29% of Cu, and 49.19% of Pb were removed when this method was used. Furthermore, the application of these three modes of the advanced removal method resulted in a decrease in the amounts of heavy metals eluted and brought about an increase in the CaO content of the treated incineration ash, while decreasing its Cl content. These combined results enhanced the solidification effect of the treated incineration ash. Thus, it was confirmed that the advanced removal method is a promising strategy by which recyclable paper incineration ash can be obtained. [ABSTRACT FROM AUTHOR]

Layered metal chalcogenide materials are exceptionally appealing in optoelectronic devices thanks to their extraordinary optical properties. Recently, their application as flexible and wearable photodetectors have received a lot of attention. Herein, broadband and high-performance paper-based PDs were established in a very facile and inexpensive method by rubbing molybdenum disulfide and titanium trisulfide crystals on papers. Transferred layers were characterized by SEM, EDX mapping, and Raman analyses, and their optoelectronic properties were evaluated in a wavelength range of 405–810 nm. Although the highest and lowest photoresponsivities were respectively measured for TiS3 (1.50 mA/W) and MoS2 (1.13 μA/W) PDs, the TiS3–MoS2 heterostructure not only had a significant photoresponsivity but also showed the highest on/off ratio (1.82) and fast response time (0.96 s) compared with two other PDs. This advantage is due to the band offset formation at the heterojunction, which efficiently separates the photogenerated electron–hole pairs within the heterostructure. Numerical simulation of the introduced PDs also confirmed the superiority of TiS3–MoS2 heterostructure over the other two PDs and exhibited a good agreement with the experimental results. Finally, MoS2 PD demonstrated very high flexibility under applied strain, but TiS3 based PDs suffered from its fragility and experience a remarkable drain current reduction at strain larger than ± 0.33%. However, at lower strains, all PDs displayed acceptable performances. [ABSTRACT FROM AUTHOR]

The increasing prevalence of antibiotic resistance threatens to make currently treatable bacterial diseases deadly again. As drug resistance rises, antibiotic susceptibility testing needs to adapt to allow for widespread, individualized testing. Paper-based diagnostics offer low-cost, disposable alternatives to traditional time consuming and costly in-house methods. Here, we describe a paper-based microfluidic device, called the Bac-PAC, capable of categorizing the antibiotic susceptibly of individual strains of Carbapenem-resistant Enterobacterales. Each chip provides a colored readout with actionable susceptibility classification of three antibiotics, thus maximizing the chances of identifying a viable therapy. We verified the technology on thirty bacterial strains with two dyes using six clinically relevant antibiotics. We demonstrated that the dried tests are stable for one month and can be incubated in a rechargeable coffee mug that reduces the need for external infrastructure. [ABSTRACT FROM AUTHOR]

In this study, we introduce a uricase-immobilized paper (UOx--paper) integrated electrochemical sensor for detection of uric acid (UA) in saliva. The UOx was immobilized on the detection zone in the wax-patterned paper substrate. This UOx-paper was integrated with a Prussian blue--modified, screen-printed carbon electrode after electropolymerization of o-phenylenediamine to construct an electrochemical cell for small-volume (20 μL) of samples. First, we optimized the fabrication conditions of UOx-paper. Next, the amperometric response of the UOx-paper-based electrochemical UA sensor was analyzed using a known concentration of UA standard solution in artificial saliva at an applied potential of − 0.1 V (versus Ag pseudo-reference electrode). The UOx--paper based electrochemical UA sensor showed a sensitivity of 4.9 μA·mM−1 in a linear range of 50 to 1000 μM (R2 = 0.998), high selectivity and good reproducibility, as well as a limit of detection of 18.7 μM (0.31 mg/dL) UA. Finally, we quantified the UA levels in undiluted saliva samples of healthy controls (n = 20) and gout patients (n = 8). The levels were correlated with those measured with conventional salivary UA enzymatic assays as well as serum UA levels. The UOx-paper-based electrochemical UA sensor is a user-friendly and convenient tool to assess salivary UA levels. [ABSTRACT FROM AUTHOR]

Pharmacists are expected to demonstrate their expertise in clinical practice and conduct research activities to generate new evidence. However, the factors promoting research activities among pharmacists remain unclear. Therefore, we investigated the research activities of Japanese pharmacists through a questionnaire survey and examined the factors contributing to the promotion of research activities. A web-based questionnaire using Google Forms was disseminated across pharmacists working in community pharmacies, drugstores, hospitals, and clinics. The questionnaire included respondents' backgrounds, research activities, and research environments. Logistic regression analysis was used to examine the factors promoting pharmacists' research activities, with experience in research paper acceptance as the objective variable. In total, 401 responses were included in the analysis. Of the respondents, 54.1% were hospital pharmacists, and 77.1% were pharmacists with > 5 years of pharmacist experience. Furthermore, 50.4% of the pharmacists had presented at conferences, and 22.2% had experience in research paper acceptance. The influential factors were "master's degree or higher," "number of affiliated academic societies," "acquisition of specialists/certified pharmacists," and "daily availability of a consultant for writing research papers." This study revealed the factors contributing to the promotion of research activities among pharmacists. We believe that our findings will help promote research among pharmacists. [ABSTRACT FROM AUTHOR]

In this study, the performance of a paper-based, screen-printed biofuel cell with mesoporous MgO-templated carbon (MgOC) electrodes was improved in two steps. First, a small amount of carboxymethyl cellulose (CMC) was added to the MgOC ink. Next, the cathode was modified with bilirubin prior to immobilizing the bilirubin oxidase (BOD). The CMC increased the accessibility of the mesopores of the MgOC, and subsequently, the performance of both the bioanode and biocathode. CMC also likely increased the stability of the electrodes. The pre-modification with bilirubin improved the orientation of the BOD, which facilitated direct electron transfer. With these two steps, an open circuit potential of 0.65 V, a maximal current density of 1.94 mA cm−2, and a maximal power density of 465 μW cm−2 was achieved with lactate oxidase as bioanode enzyme and lactate as fuel. This is one of the highest reported performances for a biofuel cell. [ABSTRACT FROM AUTHOR]

Asymptomatic visceral leishmaniasis cases increase continuously, particularly among patients with HIV who are at risk to develop further symptoms of leishmaniasis. A simple, sensitive and reliable diagnosis is crucially needed due to risk populations mostly residing in rural communities with limited resources of laboratory equipment. In this study, a highly sensitive and selective determination of Leishmania among asymptomatic patients with Leishmania/HIV co-infection was achieved to simultaneously interpret and semi-quantify using colorimetric precipitates (gold-nanoparticle probe; AuNP-probe) and fluorescence (SYBR safe dye and distance-based paper device; dPAD) in one-step loop-mediated isothermal amplification (LAMP) assay. The sensitivities and specificities of 3 detection methods were equivalent and had reliable performances achieving as high as 95.5%. Detection limits were 102 parasites/mL (0.0147 ng/µL) which were 10 times more sensitive than other related studies. To empower leishmaniasis surveillance as well as prevention and control, this dPAD combined with SYBR safe and gold nanoparticle probe LAMP assay is reliably fast, simple, inexpensive and practical for field diagnostics to point-of-care settings in resource-limited areas which can be set up in all levels of healthcare facilities, especially in low to middle income countries. [ABSTRACT FROM AUTHOR]

Milk adulteration is a common problem in developing countries, and it can lead to fatal diseases in humans. Despite several studies to identify different adulterants in milk samples, the effects of multiple adulterants remain unexplored. In this work, a three-dimensional (3D) paper-based microfluidic device is designed and fabricated to simultaneously detect multiple chemical adulterants in milk. This device comprises a top cover, a bottom cover, and a middle layer composed of transportation and a detection zone. By making cuts on the middle layer's support, the device's flow path is characterised by optimum and uniform velocity. For the first time, seven adulterants (urea, detergents, soap, starch, hydrogen peroxide, sodium-hydrogen-carbonate, and salt) are detected in the milk sample simultaneously with specificity evaluation and detailed color interference analysis. Only 1–2 mL of sample volume is required to detect 7 adulterants at one time. We have used only 10 μ L of the reagent's volume for the colorimetric reaction and found the results within a few seconds. Observation reveals that the limit of detection (LOD) of the adulterants lies in the range between 0.05 % (vol./vol.) to 0.2 % (vol./vol.) using the colorimetric detection technique. The unknown quantity of the added adulterants is measured using the calibration curves obtained from the experiments results. The repeatability and reproducibility of the process, sensitivity, and the linear range of detection of the calibration curves and the statistical study of the color intensity data are thoroughly analysed herein. In any resource-limited setting, this simple, portable, and user-friendly 3D microfluidic device is expected to be used for testing liquid foods before consumption. [ABSTRACT FROM AUTHOR]

As members of a social species, we spend most of our time interacting with others. In interactions, we tend to mutually align our behavior and brain responses to communicate more effectively. In a semi-computerized version of the Rock-Paper-Scissors game, we investigated whether people show enhanced interpersonal neural synchronization when making explicit predictions about others' actions. Across four experimental conditions, we measured the dynamic brain activity using the functional near-infrared spectroscopy (fNIRS) hyperscanning method. Results showed that interpersonal neural synchrony was enhanced when participants played the game together as they would do in real life in comparison to when they played the game on their own. We found no evidence of increased neural synchrony when participants made explicit predictions about others' actions. Hence, neural synchrony may depend on mutual natural interaction rather than an explicit prediction strategy. This study is important, as it examines one of the presumed functions of neural synchronization namely facilitating predictions. [ABSTRACT FROM AUTHOR]

Social insects are highly diverse in their social structures, aside from the consistent presence of reproductive castes. Among social insects, the Australian paper wasp Ropalidia plebeiana constructs extremely dense colony aggregations consisting of hundreds of colonies within a few square meters; however, little is known about the aggregation structures. We genetically analyzed the colony and population structure of R. plebeiana, and concomitant variations in colony sex ratios. In spring, the foundress (candidate queen) group started their colonies on a single old comb from the previous season, subsequently dividing these old combs via relatedness-based comb-cutting. Female philopatry, a prerequisite condition of Local Resource Competition (LRC), was confirmed. The colony sex ratio of reproductive individuals (male and female offspring for the next generation) became slightly male-biased in larger colonies, as predicted under LRC. However, the number of foundresses was positively associated with the number of reproductive individuals, suggesting that Local Resource Enhancement (LRE) also operates. Although the population structure appears to meet the prerequisites of LRC, the sex ratio appears to be modulated by factors other than LRC. Rather, through LRE, the availability of female helpers at the founding stage is likely to mitigate the sex ratios predicted under LRC. [ABSTRACT FROM AUTHOR]

Oxazolidine is a new category of stimuli-chromic organic compounds with unique characteristics in response to polarity, pH changes, water, light, and metal ions that were well-known as solvatochromism, acidochromism, hydrochromism, photochromism, and ionochromism, respectively. Therefore, oxazolidine derivatives have been developed for their potential applications in chemosensors, anticounterfeiting, and rewritable hydrochromic papers. In this study, various oxazolidine derivatives containing hydroxyl and naphthalene substituted groups were synthesized by using two different indolenine compounds. The synthesized oxazolidine derivatives were used for investigation of solvatochromism in different solvents, and also acidochromism in various pHs by using UV–Vis and fluorescence spectroscopies. In addition, the oxazolidine derivatives were coated on cellulosic papers using a layer-by-layer strategy to develop rewritable acidochromic papers for printing of security tags on cellulosic papers by using acidic and alkaline solutions as water-based inks. Therefore, the developed rewritable acidochromic papers could be used as security papers. [ABSTRACT FROM AUTHOR]