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Despite the extensive use of the Internet, printed newspapers remain a primary information source. In this study, reading a newspaper in a relatively confined or poorly ventilated indoor space was simulated to determine the profile of particles released from the newspaper into the air. The consecutive simulated conditions were reading without agitation of the newspaper (NoAg), followed by reading with agitation of the newspaper (Ag) and post-reading absent the newspaper (PostR), repeated with four newspapers. We found that particle number concentration (ΣN) fell during Ag owing to electroadhesion of ultrafine particles (<200 nm) caused by static charges created by friction between the paper surface and the air as a result of newspaper agitation. Conversely, particle surface area concentrations (ΣA) and particle volume concentrations (ΣV) increased significantly during Ag. This was because the larger, fine (1-2.5 μm) and coarse mode (2.5-10 μm), particles were detached from the newspaper during agitation due to inertial detachment - the release of even a small number of these particles contributing greatly to ΣA and ΣV. The critical particle number diameter (CPND) occurred at 207-310 nm. Particles smaller than this were subject to electroadhesion during Ag. The critical particle volume diameter (CPVD) occurred at 130-497 nm. Particles larger than this were subject to inertial detachment during Ag. These observations indicate that the electroadhesion of smaller particles and the inertial detachment of larger particles occur simultaneously. Particle mass concentrations were found to be as high as 168.7-534.3 μg m -3 . However, these findings of high potential concentrations were based on the measurement in relative small micro-environment. The inhalation of such concentrations is a health risk for people who regularly read newspapers in a relatively confined or poorly ventilated indoor space., (Copyright © 2018 Elsevier B.V. All rights reserved.)

The vision of a sustainable and safe use of chemicals to protect human health, preserve the environment and maintain the ecosystem requires innovative and more holistic approaches to risk assessment (RA) in order to better inform decision making. Integrated risk assessment (IRA) has been proposed as a solution to current scientific, societal and policy needs. It is defined as the mutual exploitation of environmental risk assessment (ERA) for human health risk assessment (HHRA) and vice versa in order to coherently and more efficiently characterize an overall risk to humans and the environment for better informing the risk analysis process. Extrapolating between species which are relevant for HHRA and ERA requires a detailed understanding of pathways of toxicity/modes of action (MoA) for the various toxicological endpoints. Significant scientific advances, changes in chemical legislation, and increasing environmental consciousness have created a favourable scientific and regulatory environment to develop and promote the concept and vision of IRA. An initial proof of concept is needed to foster the incorporation of IRA approaches into different chemical sectorial regulations and demonstrate their reliability for regulatory purposes. More familiarity and confidence with IRA will ultimately contribute to an overall reduction in in vivo toxicity testing requirements. However, significant progress will only be made if long-term support for MoA-related research is secured. In the short term, further exchange and harmonization of RA terminology, models and methodologies across chemical categories and regulatory agencies will support these efforts. Since societal values, public perceptions and cultural factors are of increasing importance for the acceptance of risk analysis and successful implementation of risk mitigation measures, the integration of socio-economic analysis and socio-behavioural considerations into the risk analysis process may help to produce a more effective risk evaluation and consideration of the risks and benefits associated with the use of chemicals., (Copyright © 2015 Elsevier B.V. All rights reserved.)

The speciations of EDTA and DTPA in process, waste and river waters are modelled and simulated, specifically to the mode of occurrence in the pulp and paper mill effluents and subsequently in receiving waters. Due to relatively short residence times in bleaching process and waste water treatment and slow exchange kinetics, it is expected that the thermodynamic equilibrium is not necessarily reached. Therefore, the initial speciation plays a key role. As such, the simulations have been extended to the process waters of the pulp and paper industry taking into account estimated average conditions. The results reveal that the main species are; Mn and Ca complexes of EDTA and DTPA in pulp mill process waters; Fe(III) and Mn complexes of EDTA and DTPA in waste waters; Fe(III) and Zn complexes of EDTA and DTPA in receiving waters. It is also shown how the increasing concentration of complexing agents effects the speciation. Alkaline earth metal chelation plays a significant role in the speciation of EDTA and DTPA when there is a noticeable molar excess of complexing agents compared with transition metals.

Environmental DNA (eDNA) is an emerging biological monitoring tool that can aid in assessing the effects of forestry and forest manufacturing activities on biota. Monitoring taxa across broad spatial and temporal scales is necessary to ensure forest management and forest manufacturing activities meet their environmental goals of maintaining biodiversity. Our objectives are to describe potential applications of eDNA across the wood products supply chain extending from regenerating forests, harvesting, and wood transport, to manufacturing facilities, and to review the current state of the science in this context. To meet our second objective, we summarize the taxa examined with targeted (PCR, qPCR or ddPCR) or metagenomic eDNA methods (eDNA metabarcoding), evaluate how estimated species richness compares between traditional field sampling and eDNA metabarcoding approaches, and compare the geographical representation of prior eDNA studies in freshwater ecosystems to global wood baskets. Potential applications of eDNA include evaluating the effects of forestry and forest manufacturing activities on aquatic biota, delineating fish-bearing versus non fish-bearing reaches, evaluating effectiveness of constructed road crossings for freshwater organism passage, and determining the presence of at-risk species. Studies using targeted eDNA approaches focused on fish, amphibians, and invertebrates, while metagenomic studies focused on fish, invertebrates, and microorganisms. Rare, threatened, or endangered species received the least attention in targeted eDNA research, but are arguably of greatest interest to sustainable forestry and forest manufacturing that seek to preserve freshwater biodiversity. Ultimately, using eDNA methods will enable forestry and forest manufacturing managers to have data-driven prioritization for conservation actions for all freshwater species., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Dissolved organic carbon (DOC) components can be highly variable in aquatic ecosystems, and play a pivotal role in the global carbon cycles. To comprehend potential effects of nutrient enrichment on portion of DOC biodegradability (%BDOC), we conducted an extensive investigation on 26 urban lakes in a major metropolitan area in subtropical China in a small gradient of trophic levels from mesotrophic to light and middle eutrophic. In addition to field measurements on lake ambient conditions and laboratory analysis of DOC characteristics, we conducted a 28-day temperature-controlled incubation experiment, in which %BDOC of lake waters was determined. In the mesotrophic waters, %BDOC ranged from 0.6 to 41.4 % (11.2 ± 8.9 %). The %BDOC levels spanned from 5.2 to 20.2 % (10.7 ± 4.0 %) in the light eutrophic waters, and the %BDOC ranged from 2.7 to 35.0 % (13.7 ± 8.4 %) in the middle eutrophic waters. We found a significant change in DOC chemical composition across the study lakes characterized by shifting of trophic levels. Although the experiment found significant changes in the factors that can influence %BDOC, a significant difference was not observed in %BDOC among the three trophic levels. The %BDOC was primarily influenced by the inherent DOC concentration and aromaticity, with eutrophication leading to the varied driving factors of %BDOC in lake systems. We show that most of the lake water DOC was stable. The findings indicate the intricate interplay between biological metabolism and nutrient availability governing %BDOC dynamics in urban lake ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Understanding the current situation and risk of environmental contamination by anti-influenza drugs in aquatic environments is key to prevent the unexpected emergence and spread of drug-resistant viruses. However, few reports have been focused on newer drugs that have recently been introduced in clinical settings. In this study, the behaviour of the prodrug baloxavir marboxil (BALM)-the active ingredient of Xofluza, an increasingly popular anti-influenza drug-and its pharmacologically active metabolite baloxavir (BAL) in the aquatic environment was evaluated. Additionally, their presence in urban rivers and a wastewater treatment plant (WWTP) in the Yodo River basin was investigated and compared with those of the major anti-influenza drugs used to date (favipiravir (FAV), peramivir (PER), laninamivir (LAN), and its active metabolite, laninamivir octanoate (LANO), oseltamivir (OSE), and its active metabolite, oseltamivir carboxylate (OSEC), and zanamivir (ZAN)) to comprehensively assess their environmental fate in the aquatic environment. The results clearly showed that BALM, FAV, and BAL were rapidly degraded through photolysis (2-h, 0.6-h, and 0.4-h half-lives, respectively), followed by LAN, which was gradually biodegraded (7-h half-life). In addition, BALM and BAL decreased by up to 47 % after 4 days and 34 % after 2 days of biodegradation in river water. However, the remaining conventional drugs, except for LANO (<1 % after 10 days), were persistent, being transported from the upstream to downstream sites. The LogK d values for the rates of sorption of BALM (0.5-1.6) and BAL (1.8-3.1) on river sediment were higher than those of conventional drugs (-0.5 to 1.7). Notably, all anti-influenza drugs were effectively removed by ozonation (>90-99.9 % removal) after biological treatment at a WWTP. Thus, these findings suggest the importance of introducing ozonation to reduce pollution loads in rivers and the environmental risks associated with drug-resistant viruses in aquatic environments, thereby promoting safe river environments., Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have influenced the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In this study, we compared a wide range of cell-based bioassays to the use of chemical analysis followed by exposure-activity ratio (EAR) and Toxicological Prioritization index (ToxPi) for prioritizing chemicals, sites, and hazard concerns in water samples. Surface water samples were collected from nine sites in three Central Pennsylvania streams and analyzed for a forty-six contaminants of emerging concern (CECs), including pesticides, personal care products, and pharmaceuticals. Cell-based reporter assays evaluated human and zebrafish molecular initiating events (MIEs) in endocrine and metabolic disruption, altered lipid metabolism, and oxidative stress. Bioassays showed that 12 out of 40 assays had at least one site with activity over the effect-based trigger (EBT) values. The receptors that exhibited the highest number of samples above the EBT that would be expected to cause toxicity were Aryl hydrocarbon receptor (AhR, human and zebrafish), Pregnane X Receptor (PXR), Estrogen Receptor-beta (ERB), and Androgen Receptor (AR). Characterizing the collection sites by their bioactivity aligned closely with the stream in which samples were collected. The sum of all EARs for each chemical indicated that the pharmaceutical Carbamazepine and the pesticides Carbaryl and Atrazine posed the greatest concern. However, predicted activity and site prioritization based on individual chemical analysis and calculated EAR were different than those measured by bioassay, indicating that biologically active chemicals are present in the samples that were not included in the targeted analytes. Taken together, these data show that chemical analysis and EAR analysis are beneficial for prioritization of chemicals, whereas mechanism-based bioassays are more inclusive of known as well as unknown chemical contaminants and thus of more use for overall water quality analysis and site prioritization., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: John P. Vanden Heuvel reports financial support was provided by INDIGO Biosciences Inc. J.P. Vanden Heuvel is a Founder and Chief Scientific Officer of INDIGO Biosciences, Inc. For other authors there are no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Run-of-river (ROR) dams, often perceived as having minimal environmental impact, can induce significant hydrodynamic changes that alter aquatic ecosystems. We investigated the impacts of an ROR dam on the Madeira River, the largest Amazon tributary, focusing on phytoplankton communities, their ecological implications, and related environmental factors. Our study examined changes in biomass and environmental factors (using General Linear Mixed Models - GLMM), species composition (using PERMANOVA) before and after damming, in both the main channel and tributaries (N = 549 samples). We also identified indicator species associated with different damming phases and regions through an indicator value analysis. The results showed that, following dam construction, the phytoplankton community changed in both the main channel and tributaries, with a shift from lotic diatoms to lentic phytoflagellates. This transition was likely facilitated by altered hydrodynamics and possibly influenced by the decomposition of flooded vegetation in the dam's influence zone. The decomposition of this vegetation could explain both the observed increase in oxygen consumption and the subsequent rise in phytoflagellate biomass after damming. However, despite the overall increase in phytoplankton biomass, the values remained within oligotrophic to mesotrophic conditions, consistent with the low nutrient concentrations recorded. However, we caution that the dam-created hydrodynamic conditions are optimal for phytoplankton growth, potentially exacerbating nutrient-related issues in the future. We recommend proactive management strategies to prevent nutrient enrichment from activities such as agriculture and livestock in isolated Amazon areas affected by dams, thereby mitigating potential degradation of water quality linked to increased phytoplankton biomass., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests. Alfonso Pineda reports financial support was provided by National Council for Scientific and Technological Development. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Widespread use of plastics in consumer products, packaging, cosmetics, and industrial and agricultural production has resulted in the ubiquitous occurrence of microplastics in terrestrial environment. Compared to the marine environment, only limited studies have investigated the microplastics pollution and associated risk in terrestrial environment. The present review summarizes the global distribution of microplastics in terrestrial environment, their transport pathways and fate, risk to ecosystem and human health, and abatement strategies. Small particle sizes (<500 μm); fragment, fiber, and film shapes; transparent and white color; polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET) polymers were the major characteristics of the microplastics found in terrestrial environment. Microplastics in soils negatively affect soil organisms, while the impact of microplastics in terrestrial environment on human health is poorly understood, which needs to be explored further as there is clear evidence on their presence in human bodies. The removal of microplastics from soil environment is quite complex and costly, thus prevention of their releases is preferable. Among the existing abatement options, biodegradation, which harnesses bacterial strains to degrade microplastics through enzymatic hydrolysis, hold promise for terrestrial environment. Strengthening global cooperation, implementing timely policies on plastic use and recycle, and developing new technologies for control of microplastics are recommended to reduce the pollution in terrestrial environment. Global effort on reducing plastic wastes and enhancing their management is imperative, while substitution with biodegradable plastics could help minimize future accumulation of microplastics in terrestrial environment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The dramatical increase of sulfur concentration in eutrophic lakes, especially sulfate (SO 4 2- ), has brought attention to the impact on the lake ecosystem; however, the mechanisms driving the intensification of eutrophication and the role of SO₄ 2- concentrations remain poorly understood. To assess the impact of eutrophication on SO 4 2- dynamics in lakes, this study monitored SO 4 2- concentrations in water and sediments across seven lakes with varying trophic statuses on a spatial scale, and in the eutrophic Lake Taihu over one year on a temporal scale, as well as a series of microcosms with different initial SO 4 2- concentrations. Exogenous sulfur input is the primary driver of increased SO 4 2- concentrations in lakes, the highest SO 4 2- concentration in overlying water was 100 mg/L, as well as which reached 310.9 mg/L in sediment. The concurrent input of nutrients such as nitrogen and phosphorus exacerbated eutrophication, resulting in the destabilization of the sulfur cycle. Eutrophication promoted the SO 4 2- concentration on the spatio-temporal scale, especially in sediment, and trophic lake index (TLI) showed a positive correlation with the SO 4 2- in sediments (R 2  = 0.99; 0.88). The SO 4 2- concentration in water and TLI showed a nonlinear correlation on the temporal scale (R 2  = 0.44), and showed a positive correlation on the spatial scale (R 2  = 0.49). Microscopic experiments demonstrate that the anaerobic environment created by cyanobacteria decomposition induced sulfate reduction and significantly reduces SO 4 2- concentrations. Concurrently, the anaerobic environment facilitates the coupling of iron reduction with sulfate reduction, leading to a substantial increase in Acid Volatile Sulfides (AVS) in the sediment. These findings reveal that eutrophication has a dual effect on the dynamic change of SO 4 2- concentrations in overlying water, which is helpful to accurately evaluate and predict the change of SO 4 2- concentrations in lakes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Antibiotics play an essential role in the aquaculture industry, but their overuse and weak degradability inevitably lead to light to severe residues in natural and aquaculture environments. Most studies were interested in the occurrence, distribution, and ecological risks of a limited number of antibiotics in natural environments (rivers, lakes, and coastal regions) with a minor focus on antibiotic presence in either water, sediments, or organisms in aquaculture environments located in specific regions. In this study, we conducted a comprehensive investigation into the occurrence and distribution of up to 32 antibiotics [including 15 quinolones (QNs) and 17 sulfonamides (SAs)] in organisms and their corresponding environmental matrices from 26 freshwater aquaculture ponds in Northeast Zhejiang, China. A total of 13, 9, 7, and 7 antibiotics were detected in pond water, sediments, feeds, and aquaculture organisms, respectively, with concentration ranges of 0.6-92.2 ng/L, 0.4-1169.3 ng/g dw,

Wastewater surveillance for COVID-19 and other pathogens has expanded globally. Rapid development and availability of various assays has facilitated swift adoption of wastewater surveillance in localities with diverse requirements. However, it presents challenges in comparing data due to methodological variations. Using surrogates for recovery control to address quantification biases has limitations as the recovery of surrogates and target pathogens often diverges significantly. Using non-spiked field-obtained wastewater samples as reference samples in an inter-lab study, this article proposes a straightforward, inexpensive, and most representative way of measuring relative quantification biases that occurs in analyzing field wastewater samples. Five labs participated in the study, testing five types of assays, resulting in a total of seven methods of lab-assay combinations. Each method quantified the concentration of SARS-CoV-2 and pepper mild mottle virus (PMMoV) RNAs in two types of reference samples. The results showed significant variations in quantification among methods, but the relative quantification biases were consistent across reference samples. This suggests that relative quantification biases measured with the reference samples are contingent on methods rather than wastewater samples, and that the once-determined method-specific factors can be used to correct for quantification biases in routine wastewater surveillance results. Subsequent data standardization was performed on year-long observational data from seven cities, serving as a preliminary validation of the proposed approach. This process demonstrated the potential for quantitative data comparison through the bias correction factors obtained in this inter-lab study., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Noriko Endo reports a relationship with AdvanSentinel that includes: speaking and lecture fees. Noriko Endo reports a relationship with Shimadzu Corporation that includes: consulting or advisory and employment. Noriko Endo reports a relationship with NJS Co., Ltd. that includes: consulting or advisory. Noriko Endo reports a relationship with JNC Corporation that includes: consulting or advisory. Masaaki Kitajima reports a relationship with Shionogi and Co Ltd that includes: funding grants. Masaaki Kitajima reports a relationship with AdvanSentinel that includes: funding grants. Masaaki Kitajima reports a relationship with Shimadzu Corporation that includes: funding grants. Masaru Ihara reports a relationship with Shimadzu Corporation that includes: funding grants. Masaru Ihara reports a relationship with Shimadzu Techno-Research Inc that includes: consulting or advisory. Masaru Ihara reports a relationship with Hiyoshi Corporation that includes: consulting or advisory. Hiroaki Tanaka reports a relationship with Tokyo Engineering Consultant Ltd. that includes: employment. Hiroaki Tanaka reports a relationship with Osaka Science & Technology Center that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. Hiroaki Tanaka reports a relationship with Water Reuse Promotion Cente that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. Hiroaki Tanaka reports a relationship with Association of Water and Sewage Works Consultant Japan that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. Hiroaki Tanaka reports a relationship with Japan Sewage Works Association that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Japan Wastewater Surveillance Association that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Ministry of Land Infrastructure Transport and Tourism Japan that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Government of Japan Ministry of the Environment that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Government of Japan Ministry of Health, Labor and Welfare that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Environmental Restoration and Conservation Agency that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Japan Institute of Wastewater Engineering and Technology that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Japan Water Agency that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Water Resources Environment Center that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with New Energy and Industrial Technology Development Organization that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Japan Science and Technology Agency that includes: funding grants and paid expert testimony. Hiroaki Tanaka reports a relationship with Japan Society for the Promotion of Science that includes: funding grants and paid expert testimony. Hiroaki Tanaka reports a relationship with Kyoto Prefecture that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Shizuoka Prefecture that includes: consulting or advisory and travel reimbursement. Hiroaki Tanaka reports a relationship with Kurita Water and Environment Foundation that includes: board membership, consulting or advisory, paid expert testimony, and travel reimbursement. Hiroaki Tanaka reports a relationship with Kyoto University that includes: travel reimbursement. Hiroaki Tanaka reports a relationship with The University of Tokyo that includes: paid expert testimony and travel reimbursement. Hiroaki Tanaka reports a relationship with Tsinghua University that includes: paid expert testimony. Hiroaki Tanaka reports a relationship with Kanazawa University that includes: paid expert testimony, speaking and lecture fees, and travel reimbursement. Hiroaki Tanaka reports a relationship with WOTA CORP. that includes: consulting or advisory and funding grants. Hiroaki Tanaka reports a relationship with Public Works Research Institute that includes: paid expert testimony and travel reimbursement. Hiroaki Tanaka reports a relationship with National Institute of Environmental Health Sciences that includes: consulting or advisory and travel reimbursement. Masaaki Kitajima has patent pending to Shionogi and Co Ltd. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Organic additives are incorporated during the manufacturing of plastics, and these additives are gradually released into the environment from plastic debris. Among these, phthalate acid esters (PAEs) are the most prevalent. PAEs can be found in the atmosphere, aquatic ecosystems, terrestrial regions, soil, and within animal and human bodies. They are released from industrial activities and have a significant impact on the natural environment. This study reviews research on PAEs from various regions worldwide, with about 47.8 % of the studies published between 2020 and 2024. The highest concentrations of PAEs were detected in fish samples from rivers in Taiwan, ranging from 13.6 to 70.0 mg/kg dry weight. PAEs tend to accumulate more in benthic organisms and sediments. DEHP was the most prevalent PAE in fish samples, showing the highest levels and detection frequency among the analyzed PAEs. Some studies found a strong correlation (r 2  = 0.85) between PAEs concentrations in fish and water. The findings of this study can help in assessing the fate and behavior of PAEs in the environment and provide a basis for developing future management strategies to control phthalate acid esters pollution in aquatic environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

This study provides the first systematic data on the distribution of short- (SCCPs) and medium chlorinated paraffins (MCCPs) in the atmosphere and surface soils of Lahore, Pakistan. The spatial distribution of SCCPs and MCCPs in air (n = 12) and soil (n = 15) was investigated from industrial, residential, commercial, conventional e-waste burning and background areas of Lahore. The concentrations ranged from below the limit of detection (3 . Higher CPs contaminated soil and air were observed in industrial and commercial areas likely due to the use of CPs as flame retardants/plasticizers in products and metalworking. Total organic carbon (TOC), SCCPs and MCCPs in soil were not correlated with each other however, a significant association between SCCPs and MCCPs was observed in air samples. Among all the samples of soil and air C 10, Cl 7 - 8 and C 14,16 Cl 7 - 8 were the predominant congeners for SCCPs and MCCPs, respectively. The comprehensive investigation of soil-air exchange for SCCPs and MCCPs revealed approximately 67 % of fugacity fractions (ffs) above 0.7, indicating net volatilization for most of the congeners. This study also revealed a notably high emission flux for SCCPs and MCCPs compared to their deposition flux, indicating that soil acted as a major source of CPs. Additionally, the emission flux for SCCPs was higher than for MCCPs, owing to their greater volatility and transport potential. A preliminary risk assessment suggested limited risks (Risk quotient <0.02) to the soil community while, the adult and toddler community had daily intakes of 5.7 and 7.2 ng/kg/day of CPs, respectively via different pathways highlighting the hotspots with high risk areas., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Per- and polyfluoroalkyl substances (PFAS) are persistent chemicals of increasing concern to human health. PFAS contamination in water systems has been linked to a variety of sources including hydrocarbon fire suppression activities, industrial and military land uses, agricultural applications of biosolids, and consumer products. To assess PFAS in California tap water, we collected 60 water samples from inside homes in four different geographic regions, both urban and rural. We selected mostly small water systems with known history of industrial chemical or pesticide contamination and that served socioeconomically disadvantaged communities. Thirty percent of the tap water samples (18) had a detection of at least one of the 32 targeted PFAS and most detections (89 %) occurred in heavily industrialized Southeast Los Angeles (SELA). The residents of SELA are predominately Latino and low-income. Concentrations of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) ranged from 6.8 to 13.6 ng/L and 9.4-17.8 ng/L, respectively in SELA and were higher than State (PFOA: 0.007 ng/L; PFOS: 1.0 ng/L) and national health-based goals (zero). To look for geographic patterns, we mapped potential sources of PFAS contamination, such as chrome plating facilities, airports, landfills, and refineries, located near the SELA water systems; consistent with the multiple potential sources in the area, no clear spatial associations were observed. The results indicate the importance of systematic testing of PFAS in tap water, continued development of PFAS regulatory standards and advisories for a greater number of compounds, improved drinking-water treatments to mitigate potential health threats to communities, especially in socioeconomically disadvantaged and industrialized areas., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

The presence of heavy metals and metalloids (metal(loid)s) in the food chain is a global problem, and thus, metal(loid)s are considered to be Potentially Toxic Elements (PTEs). Arsenic (As), lead (Pb), mercury (Hg), and cadmium (Cd) are identified as prominent hazards related to human health risks throughout the food chain. This study aimed to carry out a source attribution for metal(loid)s in shallow topsoil of north-midlands, northwest, and border counties of the Republic of Ireland, followed by an assessment of the potential ecological and human health risks. The positive Matrix Factorization (PMF) was used for source characterization of PTEs, followed by the Monte Carlo simulation method, used for a probabilistic model to evaluate potential human health risks. The mean concentrations of prioritized metal(loid)s in the topsoil range in the order of Pb (28.83 mg kg -1 ) > As (7.81 mg kg -1 ) > Cd (0.51 mg kg -1 ) > Hg (0.11 mg kg -1 ) based on the open-source Tellus dataset. This research identified three primary sources of metal(loid) pollution: geogenic sources (36 %), mixed sources of historical mining and natural origin (33 %), and anthropogenic activities (31 %). The ecological risk assessment showed that Ireland's soil exhibits low-moderate pollution levels however, concerns remain for Cd and As levels. All metal(loid)s except Cd showed acceptable non-carcinogenic risk, while Cd and As accounted for high to moderate potential cancer risks. Potato consumption (if grown on land with elevated metal(loid) levels), Cd concentration in soil, and bioaccumulation factor of Cd in potatoes were the three most sensitive parameters. In conclusion, metal(loid)s in Ireland present low to moderate ecological and human health risks. It underscores the need for policies and remedial strategies to monitor metal(loid) levels in agricultural soil regularly and the production of crops with low bioaccumulation in regions with elevated metal(loid) levels., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Freshwater ecosystems are being degraded by a wide range of stressors resulting from human activities. Various structural and functional metrics or indices are used to assess the 'health' or condition of riverine ecosystems. It is uncertain if structural or functional metrics or indices respond to different stressors and whether some are more responsive to stressors in general. Here we conducted a multi-study synthesis, similar to a meta-analysis, across four independent outdoor mesocosm experiments involving the manipulation of various chemical stressors - two types of salinity (synthetic marine salts (SMS) and sodium bicarbonate), two insecticides (malathion and sulfoxaflor), increased nutrients (N and P), increased sedimentation and two combinations of stressors (1: malathion, nutrients and sedimentation, 2: sulfoxaflor, nutrients and sedimentation). We compare the effects of these singular or multiple stressors on stream macroinvertebrate community structure, and Eucalyptus camaldulensis leaf litter breakdown rates by microbes and total (microbes and invertebrates). Macroinvertebrate communities were adversely affected by the two sets of multiple stressors, SMS, and both insecticides yet, and in contrast to several published studies, both microbial and total leaf litter was unaffected. Nutrients and sodium bicarbonate, increased breakdown rates or had a unimodal 'Ո' shaped response, with maxima at intermediate levels. Sedimentation by fine sand, however, decreased total leaf litter breakdown, while not affecting microbial leaf litter breakdown. Divergent responses between the effects of stressors on leaf litter breakdown rates that we observed and those in the literature may be caused by multiple mechanisms, including differences between communities, functional redundancy and differences in stressor magnitude and interactions with other (unknown) variables., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

China has made enormous strides to achieve high-quality development and biodiversity conservation, and the establishment of nature-protected areas is one of the essential initiatives. Caizi Lake involves a natural reserve and two national wetland parks, accommodating winter migratory waterfowl over the middle and lower Yangtze River basin in China. However, the water transfer from the Yangtze River to the Huai River (YR-HR water transfer) has modified the winter hydrological conditions of Caizi Lake, negatively affecting wintertime waterfowl habitats. Hence, conserving wintertime waterfowl habitats necessitates knowledge of the dynamical mechanisms behind the impacts of YR-HR water transfer on wintertime waterfowl habitats and adaptive measures. Here we developed a machine learning model, the normalized difference vegetation index, and on-spot observatory datasets such as the spatial distribution of waterfowl species and underwater topography of Caizi Lake. We found that the rising winter water level of Caizi Lake encroaches on winter waterfowl habitat with extremely high suitability. Meanwhile, rising water levels reduced waterfowl food sources. Thus, rising water levels due to YR-HR water transfer deteriorated waterfowl living conditions over Caizi Lake. Therefore, we proposed adaptive measures to alleviate these negative effects, such as water level regulation, artificial feeding of waterfowls, restoration and reconstruction of contiguous mudflats, grass flats. This study highlights human interferences with waterfowl habitats, necessitating biodiversity conservation at regional scales., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

To date, in many countries the only legally valid method for evaporative cooling system (ECS) monitoring is the culture method. However, a duration of up to 14 days and a risk of underestimation of Legionella concentrations are seen as limitations of cultivation methods. Rapid cultivation-independent methods are an important step towards a more practicable monitoring of ECS to quickly control interventions if elevated concentrations of Legionella are found. Two commercial kits for quantitative polymerase chain reaction (qPCR) and viability-qPCR (v-qPCR) were studied, comprising sample filtration and DNA extraction. Cryopreserved Legionella pneumophila were established as calibration standard with intact (ILC) and total Legionella count (TLC) determined by flow cytometry before conducting spiking experiments in commercial mineral water and artificial process water. Final assessment was carried out using real ECS samples. Recovery and robustness ranged from 86 to 108 % for qPCR with a drop to 40-60 % for v-qPCR when compared to direct extraction, possibly attributable to cell damage during sample concentration. All methods including culture did perform well regarding linearity with R 2  ≥ 0.95 for most trials. Detected concentrations in comparison to spiked Legionella counts differed with culture averaging 25 ± 7 % of spiked ILC and v-qPCR being closest to spiked concentrations with 65-144 %. In comparison, qPCR was several fold above spiked TLC concentrations. For real ECS samples Legionella spp. were detected in concentrations above 10 3 GU/100 mL by v-qPCR in 70-92 % of samples, depending on the kit used. Most of these samples were either culture-negative or not evaluable on agar plates. This study showed that a cryopreserved bacterial standard based examination is applicable and can be used for future v-qPCR verification. For assessment of differences in results between culture and v-qPCR/qPCR in ECS samples expert knowledge about the operating mode and used analytical methods is required. Guidelines addressing this issue could be a solution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Sewer overflows are an environmental concern due to their potential to introduce contaminants that can adversely affect downstream aquatic ecosystems. As these overflows can occur during rainfall events, the influence of rainwater ingress from inflow and infiltration on raw untreated wastewater (influent) within the sewer is a critical factor influencing the dilution and toxicity of the contaminants. The Vineyard sewer carrier in the greater city of Sydney, Australia, was selected for an ecotoxicological investigation of a sanitary (separate from stormwater) sewerage system and a wet-weather overflow (WWO). Three influent samples were collected representing dry-weather (DW), intermediate wet-weather (IWW) and wet-weather (WW). In addition, a receiving water sample was also collected downstream in Vineyard Creek (WW-DS) coinciding with a WWO. We employed direct toxicity assessment (DTA) and toxicity identification evaluation (TIE) approaches to gain comprehensive insights into the nature and magnitude of the impact on influent from rainwater ingress into the sewer. Three standard ecotoxicological model species, a microalga, Chlorella vulgaris, the water flea, Ceriodaphnia dubia and the midge larva, Chironomus tepperi were used for both acute and chronic tests. The study revealed variable toxicity responses, with the sample of influent collected in wet-weather displaying lower toxicity compared to the dry-weather sample of influent. Ammonia, and metals, were identified in dry weather as contributors to the observed toxicity, however, this risk was alleviated through rainwater ingress in wet-weather with further dilution within the receiving water. Based on toxicity data, dilutions of influent to minimise effects on C. vulgaris and C. dubia ranged from 1 in 12 in DW to 1 in 2.8 in WW, and further diminished in the receiving water to 1 in 1.8. The successful application of ecotoxicological approaches enabled the assessment of cumulative effects of contaminants in influent, offering valuable insights into the sanitary sewer system under rainwater ingress., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Extreme precipitation can significantly influence the water quality of surface waters. However, the total amount of bacteria carried by rainfall runoff is poorly understood. Here, thirty rainfall scenarios were simulated by artificial rainfall simulators, with designed rainfall intensity ranging from 19.3 to 250 mm/h. The instantaneous concentration ranges of R2A, nutrient agar (NA) culturable bacteria, and viable bacteria in runoff depended on the types of underlying surfaces. The instantaneous bacterial concentrations in runoff generated by forest lands, grasslands and bare soil were: R2A culturable bacteria = 10 4.5-6.3 , 10 4.5-6.1 , 10 4.0-5.3 colony-forming units (CFU)/mL, NA culturable bacteria = 10 4.0-6.0 , 10 3.9-5.8 , 10 3.2-4.9 CFU/mL, and viable bacteria = 10 6.4-8.0 , 10 7.0-8.9 , 10 6.4-7.6 cells/mL. Based on the measured bacterial instantaneous concentration in runoff, cumulative dynamic models were established, and the maximum amount of culturable bacteria and viable bacteria entering water sources were estimated to be 10 9.38-11.31 CFU/m 2 and 10 11.84-13.25 cells/m 2 , respectively. The model fitting and the bacterial accumulation dynamics were influenced by the rainfall types (p < 0.01). Surface runoff from the underlying surface of forest lands and grasslands had a high microbial risk that persisted even during the "Drought-to-Deluge Transition". Bacterial accumulation models provide valuable insight for predicting microbial risks in catchments during precipitation and can serve as theoretical support for further ensuring the safety of drinking water under the challenge of climate change., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Outdoor environments extend living spaces as venues for various activities. Comfortable open public spaces can positively impact citizens' health and well-being, thereby improving the livability and resilience of cities. Considering the visitors' perception of these environments in comfort studies is crucial for ensuring their well-being and promoting the use of these spaces. However, traditional survey methods may be time- and resource-consuming to gather significant sample sizes, usually focusing on selected homogeneous samples. Crowdsourced data, then, has emerged as an alternative for assessing human perception, as it eases the collection of subjective feedback and potentially amplifies impact and inclusivity. This study presents a strategic approach for analyzing publicly available and willingly reported crowdsourced data from a digital mapping platform in outdoor comfort evaluations, aiming to verify whether these data are informative regarding environmental quality perception and to identify the environmental factors that people are most sensitive to. Urban parks located in New York City served as a case study. A multi-source, interdisciplinary information framework combined crowdsourced reviews with environmental data used to determine prevailing thermal conditions. Overall perception of parks was well-rated, revealing that their attractions and activities are probably the most appealing characteristics for park attendance. Regarding environmental perception, acoustic and thermal factors are clearly the most influential. Acoustics were well-rated, while the main aspect regarding the thermal domain is the recognition of shading as a mitigator for hot conditions. Environmental data provided complementary insights, particularly concerning the range of thermal sensations experienced in urban parks. The findings confirm that willingly reported crowdsourced data can provide valuable insights into urban crowd environmental perception, presenting a potentially suitable and effective method to include the human perspective in environmental quality assessments, as well as to evaluate and predict environmental-related risks., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)

Anthropogenic activity has disturbed the natural distribution and circulation of trace elements in the environment. This has led to increased background levels of numerous elements, causing global pollution. In this context, seabirds are relevant bioindicators of environmental contamination. This study focuses on the ecological factors that influence the concentrations of 14 trace elements in the blood of the chicks of three sympatric gull species from the French coast of the English Channel. Between 2015 and 2017, 174 birds were sampled in the industrialised Seine Estuary (in the city of Le Havre and on Ratier Island) and in the remote Chausey Islands, 200 km to the west. We also considered the Se:Hg molar ratio using Hg concentrations in those birds. Ag and V concentrations were below the quantification limit in all cases, while the fraction of non-quantified samples was higher than 30 % for Cd, Cr and Ni. Among the elements quantified in the samples, the lowest concentrations were noted for Co and the highest for Fe, building the following order: Co < Cd < Ni < Mn ≤ Pb < Cr < Hg < Cu < Se < As < Zn < Fe. No unanimous scheme of concentrations among elements, species and sites existed. Similarly, different models were fitted and different factors were significant for different species and elements. We observed the biomagnification of As and the biodilution of Pb. Pb concentrations were also highest in the industrial site in the city of Le Havre. Despite the high proportion of non-quantified samples for Cd, Cr and Ni, we continued to notice higher concentrations in the marine environment of the Chausey Islands. Concentrations of some elements clearly revealed habitat dependence. In some cases the Se:Hg molar ratio was lower than 4, a threshold for diminishing Hg toxicity by Se., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Drug consumption estimates are traditionally based on surveys or information from police seizures. Alternatively, residues of illicit drugs in untreated wastewater (influent) can be used to calculate mass loads and subsequently estimate drug consumption in the community throughout the week. For this purpose, wastewater is commonly sampled for seven consecutive days within the Sewage analysis CORe group Europe (SCORE), while other sampling schemes may be implemented in long-term studies outside this consortium. The current study demonstrates how sampling frequency of illicit drug residues in the influent of wastewater treatment plants (WWTPs) affects the derived weekly average. Thirty WWTPs were sampled over the course of 12 years and influents were analyzed for five drugs (metabolites): 3,4-methylenedioxymethamphetamine (MDMA), methamphetamine, amphetamine, benzoylecgonine (a metabolite of cocaine), and 11-nor-9-Carboxy tetrahydrocannabinol (THC-COOH). Subsequently, small and large WWTPs were grouped with a threshold of 100,000 inhabitants. After data curation, standardized loads were calculated (mg/d per 1000 inhabitants). Weekly averages of loads of the drug residues were calculated based on six scenarios (sampling one to six weekdays) and compared to the weekly average in the control situation (sampling seven weekdays) in a Monte Carlo simulation. Results indicate that drug residues with more dynamic loads over a week require more frequent sampling. The analysis illustrates that a decreased sampling frequency (4 or 5 days per week) still leads to a representative weekly average for all drugs tested when a deviation up to a factor of 1.25 is deemed acceptable. However, knowledge on typical levels is necessary to define outliers. We therefore recommend to study dynamics in drug residue loads for WWTPs before reducing sampling frequency in long term monitoring programs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

The lead‑zinc smelter at Trail (British Columbia, Canada) has operated continuously for ∼125 years, with long-standing concerns that transboundary metal(loid) and sulphur emissions have contaminated water bodies in both western Canada and Washington (WA), USA. To assess aquatic ecosystems affected by over a century of industrial contamination requires an understanding of pre-smelting conditions. Here, we use a dated sediment core from Williams Lake (WA), downwind of both the Trail and the short-lived LeRoi (Northport, WA) smelters, to track regional contaminant history and other environmental stressors. Specifically, we examine a selection of chemical elements, cladoceran assemblages, visible range spectroscopy-inferred chlorophyll a (VRS-Chl a) and visible near-infrared spectroscopy-inferred lake-water total organic carbon (VNIRS-TOC). Sedimentary proxies recorded the onset of smelting in 1896 CE, peak periods of aerial emissions in the early to mid-20th century, and the history of emission controls. With a few exceptions, sedimentary metal(loid)s exceeded Canadian Interim Sediment Quality Guidelines during the height of the smelting era and have declined substantially since ca. 2000 CE. The loss of metal-sensitive Cladocera and declines in primary production (VRS-Chl a) at the onset of the regional smelting era indicate a strong biological response to airborne industrial contamination. The largest cladoceran change in the sediment record was concurrent with accelerated mitigation efforts at the Trail facilities following the 1960s; however, this compositional shift was between ecologically similar daphniid taxa. Steep declines in VNIRS-TOC concentrations during the period of peak emissions at Trail suggested an increase in sulphur deposition on the landscape that reduced terrestrial carbon supply. However, the persistence of calcium-sensitive daphniids throughout the record indicates that alkaline Williams Lake had not acidified. Current cladoceran assemblages remain substantially distinct from pre-industrial communities, demonstrating how paleoecotoxicological approaches can be used to track the effects of multiple stressors in a temporally appropriate context., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Rivers are undergoing significant changes under the pressures of natural processes and human activities. However, characterizing and understanding these changes over the long term and from a spatial perspective have proven challenging. This paper presents a novel framework featuring twelve indicators that combine geometric and spatial structures for evaluating changes in river network patterns. Through global principal component analysis, these indicators were integrated into a comprehensive river network pattern index (RNP). Employing Pearson correlation analysis, geographically weighted regression, geographic detector models, and the Shapley Value, the study quantitatively analyzed various stressors' impacts and relative contributions on river network changes from the 1960s to 2015s. The results showed a clear trend of degradation over time, particularly with frequency and density declining by 57 % and 48 %, respectively. The changes across subbasins varied temporally and spatially, with the 1980s emerging as a significant temporal hotspot and six spatial hotspots identified among twenty subbasins. The analysis showed that agriculture was significantly negatively associated with RNP, while the relationship between urbanization and RNP was inverted N-shaped. To address the negative effects of human activities, a shift from uniform management approaches is crucial. In agricultural areas, adopting more intensive farming practices could help mitigate negative impacts on RNP. For highly urbanized regions, city planning should consider the interactions between urbanization and other factors affecting RNP. Overall, incorporating an understanding of RNP's spatial-temporal dynamics and driving factors into spatial planning is critical for creating effective and sustainable management strategies for human-river interactions., Competing Interests: Declaration of competing interest The author declares that there are no known competing financial interests or personal relationships that influenced the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Solving the challenges faced during the measurement of the cross-interface transfer of perfluoroalkyl acids (PFAAs) in lakes is crucial for clarifying environmental behaviours of these chemicals and their efficient governance. This study developed a multimedia fugacity model based on the quantitative water-air-sediment interaction (QWASI) covering abiotic/biotic matrices to investigate the cross-interface transfer and fate of PFAAs in Luoma Lake, a typical PFAA-contaminated shallow lake in eastern China. The accuracy and reliability of the established model were confirmed using Percent bias and Monte Carlo simulation, respectively. Using the QWASI model, the multimedia transfer of the PFAAs and their accumulation and persistence in different sub-compartments were described and measured, and the differences among individual PFAAs were explored. The simulation results showed that the sedimentation and resuspension of PFAAs were the most intense cross-interfacial transfers, and the sediments served as a chemical sink in the long term. A significant negative correlation of N C-F (the number of CF bonds) with the relative outflow flux (T W · out-ct ) but a positive correlation with the relative net transfer across the interface between water and aquatic plants (T p-ct ) was detected, indicating that the PFAA migration capacity decreased but the bioaccumulation potential increased with the CF bond number. The persistence in water (P w ) of individual PFAAs ranged from 19.65d (PFOA) to 32.22d (PFOS), with an average of 26.15d; their persistence in sediment (P s ) ranged from 432d (PFBA) to 3216d (PFOS), with an average of 1524d, increasing linearly with an increase in N C-F . The water advection flows into and out of the lake (Q W · in and Q W · out ), the PFAA concentration of water inflow (C W · in ), and bioconcentration factor of aquatic plants (BCF p ) were the primary parameters sensitive to PFAAs in all sub-compartments, which are essential indexes for exploring promising remediation pathways for lacustrine PFAA contamination based on the fugacity model simulation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In degraded urban habitats, nature-based solutions aim to enhance ecosystem functioning and service provision. Bivalves are increasingly reintroduced to urban environments to enhance water quality through biofiltration, yet their long-term sustainability remains uncertain. Following the restoration of the disused South Docks in Liverpool in the 1980s, natural colonization of mussels rapidly improved dock-basin water quality and supported diverse taxa, including other filter feeders. While the initial colonization phase has been well documented, there has been limited published research since the mid-1990s, despite ongoing routine water quality monitoring. Here, we assessed the long-term persistence of mussel populations, their associated biodiversity, and physico-chemical parameters of the water in Queens and Albert Docks by comparing historical (1980s to 1990s) and contemporary data from follow-up surveys (2012,2022). Following an initial period of poor water quality (high contamination and turbidity, low oxygen), the natural colonization of mussels from Albert Dock in 1988 extended throughout the South Docks. By the mid-1990s, the environment of the South Docks and its mussel populations had stabilized. The dock walls were dominated by mussels which provided important complex secondary substrate for invertebrates and macroalgae. Surveys conducted in 2012 and 2022 confirmed the continued dominance of mussels and estimates of mussel biofiltration rates confirm that mussels are continuing to contribute to maintaining water quality. A decline in salinity was observed in both docks in 2022, with evidence of recovery. While these ecosystems appear relatively stable, careful management of the hydrological regime is crucial to ensuring the persistence of mussels and resilient ecosystem service provision through biofiltration., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

China is not only the first reported place of the COVID-19 pandemic but also is the biggest microplastic emitter in the world. Nevertheless, the impact of the COVID-19 pandemic on microplastic pollution in the watersheds of China remains poorly understood. To address this, the present study conducted a data mining and multivariate statistical analysis based on 8898 microplastic samples from 23 Chinese watershed systems before and during the COVID-19 pandemic. The results showed that the COVID-19 pandemic extensively affected the abundance, colors, shapes, polymer types, and particle sizes of microplastic in Chinese watershed systems. Before and during the COVID-19 pandemic, 77.27 % of the Chinese watershed systems observed increased microplastic abundance. Moreover, the COVID-19 pandemic itself, natural conditions (such as altitude and weather), and anthropogenic factors (such as civil aviation throughput) are highly intertwined, jointly impacting the microplastic in the watersheds of China. From the perspective of ecological risks, the COVID-19 pandemic was more likely to aggravate the microplastic pollution in the middle and down reaches of the Yangtze River Watersheds. Overall, whether before or during the COVID-19 pandemic, the main watershed systems of China still stayed at a high pollution level, which rang the alarm bell that watershed systems of China had been at serious ecological risk accused of microplastic contamination., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Coastal marine ecosystems, such as coral reefs, are severely threatened by climate changes, overexploitation, and marine pollution. Particularly, environmental pollution caused by petroleum-derived substances is poorly studied in coral reefs in tropical developing countries, with a total absence of data about these contaminants in some regions. In this work, we determined the levels of conventional and unconventional PAHs in the tissue and skeleton of the coral Montastraea cavernosa in a seascape scale of the Southwest Atlantic. We sampled in 12 coral reefs adjacent to the coast along approximately 200 km. We found 14 PAHs, 2 Oxy-PAHs, and 15 Nitro-PAHs in the samples, and among them, benzo[a]pyrene, chrysene, benzo[a]anthracene, benzo[k]fluoranthene, indeno[1,2,3-c,d]pyrene and dibenz[a,h]anthracene, which are mutagenic, teratogenic and carcinogenic substances. Skeletons presented predominantly lower quantities of ∑PAHs than the respective tissue, except for the skeletons from one reef severely impacted by oil spills. The ∑PAHs levels were lower in a bay near an urbanized region than in open sea reefs. Diagnostic ratios indicate mixogenic sources, with the predominance of petrogenic origin. Our study provides the first occurrence of PAHs, Nitro-PAHs, and Oxy-PAHs distribution in corals from the Southwest Atlantic Ocean, and we expect that these data will help to evaluate any future impacts and management of this ecosystem., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Specific campaigns to detect microplastics (MPs) in the urban water cycle were carried out in three drinking water plants and two wastewater treatment plants. A self-designed sampler for MPs detection in water matrices was in this study preliminary validated and then tested in long term campaigns sampling up to 1000 L. Raw drinking water and wastewater show microplastics (MPs) concentrations of 2-11 and of 480-801 MPs/m 3 , respectively, and MPs removals of 47-78 % and of 84-98 %, correspondingly. Specific roles of chemical and physical conventional processes in microplastics removals were investigated. Solid-liquid separation, flotation and filtration are the main processes for achieving high microplastics removal. Regarding concentrated matrices, MPs concentrations in sludge samples varied in the range of 5000-500,000 MPs/m 3 . Finally, shapes, size classes and polymers' typologies were investigated in the extracted MPs. The detected sizes are mainly 0.5-0.1 mm in drinking waters while 5-1 mm in wastewaters. Wastewaters were predominated by synthetic fibers (polyester type), while drinking waters were mainly characterized by fragments and the fibers were mostly of natural origin. Finally, the results of this study supported best practices and guidelines for a representative assessment of MPs in water (sampling methods, extraction procedures, characterization and quantification)., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Bisphenols, bisphenol A diglycidyl ether (BADGE), and bisphenol F diglycidyl ether (BFDGE) are commonly used as raw materials or additives in the production of several industrial and consumer products. However, information regarding the occurrence and distribution of these industrial chemicals in freshwater ecosystem is limited. In this study, four bisphenols, six BADGEs, and three BFDGEs were determined in abiotic and biotic samples collected from the Dongjiang River basin in southern China. Among the four bisphenols, BPA was widely present in all samples analyzed including surface water (median: 1.81 ng/L), sediment (3.1 ng/g dw), aquatic plants (3.69 ng/g dw), algae (7.57 ng/g dw), zooplankton (6.17 ng/g dw), and fish muscle (5.28 ng/g dw). Among the nine BADGEs and BFDGEs analyzed, BADGE, BADGE•H 2 O, BADGE·HCl·H 2 O and BADGE•2H 2 O was found in all sample types. Although the median concentration of BADGE•2H 2 O in surface water was below LOQ, this compound was found at median concentrations of 2.61, 3.59, 1.03, 1.69, and 49.8 ng/g dw in sediment, plants, algae, zooplankton, and fish muscle, respectively. Significant positive linear correlations were found among logarithmic transformed concentrations of BPA, BADGE, BADGE•H 2 O, BADGE•HCl•H 2 O, and BADGE•2H 2 O in sediment. The bioconcentration factor (logBCF) values of BADGE, BADGE•H 2 O, BADGE•HCl, BADGE•HCl•H 2 O, BADGE•2H 2 O, and BADGE•2HCl in fish, plants, algae, and zooplankton were > 3.3 L/kg (wet weight), indicating that these chemicals possess moderate bioaccumulation potential. The estimated daily total intake of bisphenols and BADGEs through fish consumption was 75.1 ng/kg bw/day for urban adult residents. The study provides baseline information on the occurrence of bisphenols, BADGEs, and BFDGEs in a freshwater ecosystem., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Novel brominated flame retardants (NBFRs) have emerged as an alternative to traditional brominated flame retardants (BFRs) and may pose risks to the environment and human health. However, the distribution pattern of NBFRs in urbanized zones and their association with multiple socioeconomic variables have not been adequately explored. Herein, seven NBFRs were investigated in surface soil samples from Tianjin, China, a typical urbanized area. The ∑ 7 NBFRs ranged from n.d. to 101 ng/g, dry weight (dw) (mean: 12.6 ± 17.6 ng/g dw), which exhibited a relatively elevated level compared to NBFRs in soils from other regions worldwide. Decabromodiphenylethane (DBDPE) was the main contaminant, and its concentration ranged from 0.378 to 99 ng/g, dry weight (dw) (mean: 11.4 ± 17.0 ng/g dw), accounting for 81 % of the ∑ 7 NBFRs. Notably, NBFRs exhibited peak concentrations within residential zones, significantly surpassing those recorded in the remaining four regions (green, farmland, water environment and other) (p < 0.05). Furthermore, the concentration of NBFRs in the soil of the Binhai New District within Tianjin was the highest, significantly exceeding that of other administrative areas, which was closely related to the intensive industrial activities in this region. The above results indicate that human activities are a key factor affecting the concentration of NBFRs in the soil. Moreover, a variety of statistical methods were employed to investigate the correlation between socioeconomic variables and the distribution of NBFRs. The concentration of NBFRs showed a significant correlation with population density and the gross domestic product (GDP) (p < 0.05), and the incorporation of administrative regional planning into structural equation models demonstrated an indirect influence on the spatial distribution of NBFRs concentration, mediated by its impact on population density. These results emphasize the association between NBFRs contamination and the degree of urbanization, thereby providing valuable insights for assessing the exposure risk of NBFRs among urban residents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Mercury (Hg) is a naturally occurring highly toxic element which circulation in ecosystems has been intensified by human activities. Hg is widely distributed, and marine environments act as its main final sink. Seabirds are relevant bioindicators of marine pollution and chicks are particularly suitable for biomonitoring pollutants as they reflect contamination at short spatiotemporal scales. This study aims to quantify blood Hg contamination and identify its drivers (trophic ecology inferred from stable isotopes of carbon (δ 13 C) and nitrogen (δ 15 N), geographical location, chick age and species) in chicks of eight seabird species from 32 French sites representing four marine subregions: the English Channel and the North Sea, the Celtic Sea, the Bay of Biscay and the Western Mediterranean. Hg concentrations in blood ranged from 0.04 μg g -1 dry weight (dw) in herring gulls to 6.15 μg g -1 dw in great black-backed gulls. Trophic position (δ 15 N values) was the main driver of interspecific differences, with species at higher trophic positions showing higher Hg concentrations. Feeding habitat (δ 13 C values) also contributed to variation in Hg contamination, with higher concentrations in generalist species relying on pelagic habitats. Conversely, colony location was a weak contributor, suggesting a relatively uniform Hg contamination along the French coastline. Most seabirds exhibited low Hg concentrations, with 74% of individuals categorized as no risk, and < 0.5% at moderate risk, according to toxicity thresholds. However, recent work has shown physiological and fitness impairments in seabirds bearing Hg burdens considered to be safe, calling for precautional use of toxicity thresholds, and for studies that evaluate the impact of Hg on chick development., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Highly urbanized coastal ecosystems are vital in the global carbon budget. However, there are limited researches on carbon flux gradients in these nearshore areas, considering both natural and anthropogenic influences. Through on-site measurements and field samplings during wet-to-dry season in 2023, this study investigated spatial variations and factors affecting carbon fluxes, focusing on the impacts of salinity and eutrophic status in five geographically connected coastal waters of the Guangdong-Hong Kong-Macau Greater Bay Area (GBA). By estimating carbon exchange at land-sea-air interface, dominant processes in carbon dynamics were identified as well. Results showed that partial pressure of CO 2 (pCO 2 ) varied from 391 to 2290 μatm, and sea-air CO 2 exchange fluxes (FCO 2 ) ranged from -3.07 to 70.07 mmol m -2 d -1 , indicating significant geographical distinctions among five coastal waters of the GBA. The total carbon transport from rivers to these nearshore waters was approximated at 6.44 Tg C yr -1 , with the Pearl River (PR) contributing 99.7%, primarily in dissolved forms. Atmospheric CO 2 release was calculated at 0.29 Tg C yr -1 for studied five coastal waters, primarily as carbon sources, except for Dapeng Bay (DPB) as a sink. CO 2 emissions inversely correlated with salinity, yet positively with eutrophication status, particularly in river-dominated estuaries. Moreover, CO 2 flux decreased 23 times as eco-status shift from eutrophic to non-eutrophic. River plumes, terrestrial pollutant inputs, and economic structure were underlying drivers, influencing carbon species concentrations and fluxes. Elevated CO 2 concentrations in eutrophic coastal waters were mainly attributed to terrestrial carbon and nutrients inputs, supporting active biological respiration and microbial decomposition. Conversely, carbon dynamics potentially depend on the balance of respiration and photosynthesis in non-eutrophic coastal waters. This study offers high geographic precision and specificity of carbon species, and provides land-sea integration insight to understand carbon dynamic mechanisms, promoting advancements in water quality management and climate mitigation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Nowadays, two of the endocrine disrupting compounds (EDCs) in the group of alkylphenols (APs), nonylphenol (4-NP) and octylphenol (4-t-OP), have attracted great scientific and regulatory attention mainly due to concerns about their aquatic toxicity and endocrine disrupting activity. This paper investigated the occurrence, distribution behavior, fate, and removal of 4-NP and 4-t-OP in liquid and solid phases of three full-scale wastewater treatment plants (WWTPs) with different treatment technologies comparatively. In this context, (i) advanced biological WWTP, (ii) wastewater stabilization pond (WSP), and (iii) constructed wetland (CW) were utilized. In all three investigated WWTPs, the concentrations of 4-NP (219.9-19,354.4 ng/L) in raw wastewater were higher than those of 4-t-OP (13.9-2822.4 ng/L). Within the scope of annual average removal efficiencies, 4-NP was treated highly in advanced biological WWTP (93.5 %), while it was almost not treated in WSP (3.1 %) and treated with negative removal (<0 %) in CW. While 4-t-OP was treated at a similar removal rate (93.5 %) to 4-NP in advanced biological WWTP, it was treated moderately in WSP (52.5 %) and very poorly in CW (12.4 %). It has been determined that the most important removal mechanism of both 4-NP and 4-t-OP in WWTPs is biodegradation, followed by sorption onto sewage sludge. According to the mass balance performed in advanced biological WWTP, the biodegradation rates for 4-NP and 4-t-OP were found to be 70.4 % and 86.6 %, respectively, while the sorption onto sewage sludge were determined to be 23.3 % and 6.8 %. One of the critical findings obtained within the scope of the study is that while the concentrations of both metabolites, especially 4-NP, in wastewater and sewage sludge, decreased considerably under aerobic conditions, on the contrary, their concentrations increased significantly under anaerobic conditions. Both compounds were detected at higher concentrations in primary sludge compared to secondary sludge in advanced biological WWTP, while in WSP, they were determined at higher concentrations in anaerobic stabilization pond sludge compared to facultative stabilization pond sludge. Besides, it was also determined that the sorption behavior of these alkylphenols is much more dominant than desorption., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Groundwater pollution has attracted widespread attention as a threat to human health and aquatic ecosystems. However, the mechanisms of pollutant enrichment and migration are unclear, and the spatiotemporal distributions of human health risks are poorly understood, indicating insufficient groundwater management and monitoring. This study assessed groundwater quality, human health risks, and pollutant sources in the Fen River Basin(FRB). Groundwater quality in the FRB is good, with approximately 87 % of groundwater samples rated as "excellent" or "good" in both the dry and rainy seasons. Significant precipitation elevates groundwater levels, making it more susceptible to human activities during the rainy season, slightly deteriorating water quality. Some sampling points in the southern of Taiyuan Basin are severely contaminated by mine drainage, with water quality index values up to 533.80, over twice the limit. Human health risks are mainly from As, F, NO 3 - , and Cr. Drinking water is the primary pathway of risk. From 2019 to 2020, the average non-carcinogenic risk of As, F, and NO 3 - increased by approximately 28 %, 170 % and 8.5 %, respectively. The average carcinogenic risk of As and Cr increased by 28 % and 786 %, the overall trend of human health risks is increasing. Source tracing indicates As and F mainly originate from geological factors, while NO 3 - and Cr are significantly influenced by human activities. Various natural factors, such as hydrogeochemical conditions and aquifer environments, and processes like evaporation, cation exchange, and nitrification/denitrification, affect pollutant concentrations. A multi-tracer approach, integrating hydrochemical and isotopic tracers, was employed to identify the groundwater pollution in the FRB, and the response of groundwater environment to pollutant enrichment. This study provides a scientific basis for the effective control of groundwater pollution at the watershed scale, which is very important in the Loess Plateau., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

We assessed the concentrations of metals and other trace elements in two of the most common seabird species breeding on Svalbard, the black-legged kittiwake (Rissa tridactyla) and the Brünnich's guillemot (Uria lomvia). Both of these species feed mostly on fish and crustaceans but have different foraging strategies, kittiwakes being surface feeders while guillemots are divers. We investigated the concentrations of arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), mercury (Hg), lead (Pb), selenium (Se) and zinc (Zn) in the plasma and body feathers of black-legged kittiwakes (n = 17), as well as in the body feathers of Brünnich's guillemots (n = 13). Samples were collected from adult birds at two time points, one week apart during July 2017 in Kongsfjorden, Svalbard. Of the non-essential trace elements, As was found at the highest median concentration at both the first (56.23 ng/g ww) and second (39.99 ng/g ww) sampling timepoints in the kittiwake plasma. When separating for the sexes, as well as sampling time, males sampled at the first sampling time point had significantly higher concentrations of As (median at 0.087 ng/g versus 0.039 ng/g) and Se (median 0.26 ng/g versus 0.16 ng/g) compared to males sampled at the second time point. There was no significant difference in plasma concentrations between females at first and second sampling time points. Kittiwake feathers contained significantly higher concentrations of As, Cd and Hg than guillemot feathers, while guillemot feathers had significantly higher concentrations of Cu, Pb and Zn. However, of the non-essential elements in both kittiwake and guillemot feathers Hg was found with the highest median concentrations at 5160 and 1080 ng/g, respectively, thus in kittiwakes exceeding the level of 5000 ng/g associated with adverse effect (e.g., impaired reproduction). Levels of Hg and Se found in the kittiwake feathers were higher than previous studies on seabirds in the Arctic., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Sewage surveillance is widely applied to track valid human excretion information and identify public health conditions during corona virus disease 2019 (COVID-19) pandemic. This approach can be applied to monitor the antibiotic resistance level in sewers and to assess the risk of spreading antibiotic resistance in municipal wastewater systems. However, there is still little information about human antibiotic resistance occurrence characteristics in sewer system. This study conducted a field trial for whole year to advance understanding on spatial and temporal occurrence of antibiotic resistance genes (ARGs) in gravity sewerage. The spatial distribution of ARGs along the drainage pipe line (from human settlements to wastewater treatement pant (WWTP)) was insignificant, which may be affected by irregular human emission alongside the pipeline. The correlation between ARGs and antibiotics in sewage was insignificant. The temporal distribution showed that the effect of temperature on ARGs abundance was evident, the ARGs abundance in sewage was generally higher during the cold season. Metagenomic analysis revealed that the detected ARGs were mainly distributed in Proteobacteria (47.51 %) and Antinobacteria (20.11 %). Potential hosts of ARGs in sewage were mainly identified as human gut microorganisms, including human pathogenic bacteria, such as Prevotella, Kocuria, and Propionibacterium, etc. This study provides a new insight into the sewerage surveillance tracking characteristics of human ARGs in sewer system, and suggesting that the sewage-carried ARGs surveillance is a promising method for assessment and management of antibiotic resistance level on population size., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Seabirds have become biovectors of plastic pollutants between marine and terrestrial ecosystems, and transport of plastics to their nesting sites becomes relevant due to increasing levels of pollution. To determine the pathways by which plastic reaches their colonies, we analysed the abundance of plastics at the nesting sites of five seabird species (Humboldt penguin Spheniscus humboldti, Peruvian booby Sula variegata, kelp gull Larus dominicanus, grey gull Leucophaeus modestus, Markham's storm-petrel Hydrobates markhami) nesting in northern Chile. Seabirds were primarily grouped according to their nesting behaviour, but two species foraging in contrasting habitats (kelp gull and Markham's storm-petrel) were also compared directly. The abundance, type, and polymer of macro-, meso- and microplastics were analysed in the soil of colonies and control sites, and microplastic ingestion was evaluated for selected species. Densities of plastics in colonies of surface-nesting seabirds ranged from 0 to 21.4 items m -2 (mainly plastic bags and thin films), and 0.002 to 19.7 items m -2 (mainly hard fragments) in colonies of burrow-nesting seabirds. Mean microplastic loads in the stomachs of seabirds were between 3.7 ± 4.2 plastic items individual -1 . Overall, the abundances of plastic items in all seabird colonies were low, suggesting a limited transfer of plastics from sea to land. For kelp gulls, the results indicate transfer of macroplastic items to colonies, reaching the colony via regurgitates, with landfills considered as the main plastic source. Our results suggest that contrasting nesting behaviour and foraging habitats among species can explain differential plastic accumulation in seabird colonies, but also other factors, such as wind, contribute to the accumulation of plastic debris in colonies. Proper management of sanitary landfills are key to reduce plastic contamination of coastal seabirds and their colonies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The state of Florida contains over 1000 freshwater springs, fed by groundwater that provides 90 % of the drinking water for inhabitants. Freshwater springs are regarded as some of the cleanest water sources left on Earth, but recent studies regarding the extreme pervasiveness of per- and polyfluoroalkyl substances (PFAS) across the globe have called into question whether PFAS have infiltrated these vital water sources. In this study, 90 water samples (43 vents/40 runs/plus 7 additional surface samples) from 50 freshwater Florida springs were analyzed for the presence of 29 PFAS via ultra-high-performance liquid chromatography-tandem mass spectrometry. PFAS were detected in 63 % of the vent samples and 68 % of the run samples, with a total of 13 different quantifiable PFAS (>LOQ) present in at least one sample. Concentrations across samples ranged from 0.205 to 64.6 ng/L, with the most detected PFAS being perfluorobutanesulfonic acid (PFBS), perfluorooctanoic acid (PFOA), and perfluorooctanesulfonic acid (PFOS). This data highlights the presence of PFAS in Florida springs, representing a potential health concern for spring water users and drinking water consumers, and suggests the need for further research regarding the possible contamination pathways of Florida's freshwater springs., Competing Interests: Declaration of competing interest The authors declare that they have no known financial competing interests or personal relationships that would appear to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Restoration measures have been widely implemented in wetland ecosystems globally to bend the curve of biodiversity loss and restore associated ecological functions. However, assessments of the effectiveness of wetland restoration have predominantly focused on the recovery of taxonomic composition, while few studies have assessed the effectiveness of these efforts from a food web perspective. Here, we incorporated stable isotope approach to investigate trophic structure in natural and restored wetlands in Northeast China. The investigated consumers, including zooplankton, macroinvertebrates, and fish, exhibited lower δ 15 N and higher δ 13 C values in restored wetlands than in natural wetlands. Natural wetlands exhibited higher trophic positions and a wider range of trophic levels compared to restored wetlands. Primary consumers in natural wetlands relied more on particulate organic matter (POM, 42.9 % ± 24.1 %), while those in restored wetlands were more dependent on substrate organic matter (SOM, 42.3 % ± 23.9 %). Compared to natural wetlands, isotopic richness was significantly lower in restored wetlands, with smaller isotopic variation (SEAs) in basal resources, aquatic invertebrates, and fish. Our findings reveal that the recovery of trophic structures in restored wetlands lags behind that of taxonomic composition. Future restoration efforts should prioritize enhancing habitat heterogeneity and resource availability to support a diverse range of trophic levels. Monitoring trophic dynamics is essential for assessing the progress of wetland restoration and should be integrated into monitoring schemes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Anthropogenic pressures such as climate change and nutrient pollution are causing rapid changes in the marine environment. The relative influence of drivers of change on the plankton community remains uncertain, and this uncertainty is limiting our understanding of sustainable levels of human pressures. Plankton are the primary energy resource in marine food webs and respond rapidly to environmental changes, representing useful indicators of shifts in ecosystem structure and function. Categorising plankton into broad groups with similar characteristics, known as "lifeforms", can be useful for understanding ecological patterns related to environmental change and for assessing the state of pelagic habitats in accordance with the EU Marine Strategy Framework Directive and the OSPAR Commission, which mandates protection of the North-East Atlantic. We analysed 29 years of Continuous Plankton Recorder data (1993-2021) from the North-East Atlantic to examine how trends in plankton lifeform abundance changed in relation to one another and across gradients of environmental change associated with human pressures. Random forest models predicted between 57 % and 80 % of the variability in lifeform abundance, based on data not used to train the models. Observed variability was mainly explained by trends in other lifeforms, with mainly positively correlated trends, indicating bottom-up control and/or shared responses to environmental variability were prevalent. Longitude, bathymetry, mixed layer depth, the nitrogen-to‑phosphorus ratio, and temperature were also significant predictors. However, contrasting influences of environmental drivers were detected. For example, small copepod abundance increased in warmer conditions whereas meroplankton, large copepods and fish larvae either decreased or were unchanged. Our findings highlight recent changes in stratification, reflected by variation in mixed layer depth, and imbalanced nutrient ratios are affecting multiple lifeforms, impacting the North-East Atlantic plankton community. To achieve environmental improvements in North-East Atlantic pelagic habitats, it is crucial that we continue to address climate change and reduce nutrient pollution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Diverse enteric pathogens, transmitted through human and animal feces, can cause gastroenteritis. Enteric viruses, such as human Aichi virus, specifically genotype A (AiV-A), are emerging pathogens that cause illnesses even at low doses and are spreading globally. This research developed a reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay targeting the 3CD junction and a reverse transcription colorimetric loop-mediated isothermal amplification (RT-cLAMP) duplex assay targeting junctions 2BC and 3CD of the AiV-A genome for rapid and sensitive detection of this virus in metropolitan and regional wastewater samples in Queensland, Australia. The performance of these assays was evaluated using control materials and by analyzing wastewater samples. In serially diluted control materials, RT-qPCR provided quantifiable data (mean 1.51 log 10 GC/2 μL of nucleic acid) down to a dilution of 1 × 10 -5  pg/μL. In comparison, the duplex RT-cLAMP assay detected down to 1 × 10 -4  pg/μL, indicating that its sensitivity was one order of magnitude less than that of RT-qPCR. Of the 38 wastewater samples from 38 metropolitan and regional wastewater treatment plants (WWTPs) in Queensland, Australia, 21 (55.3 %) tested positive by RT-qPCR with concentrations ranging from 3.60 to 6.23 log 10 GC/L. In contrast, only 15 (39.5 %) of 38 wastewater samples were positive using the duplex RT-cLAMP assay. The methods demonstrated substantial qualitative agreement (κ = 0.730), with a concordance of 86.5 %, demonstrating the reliability of RT-cLAMP for detecting AiV-A in wastewater samples. The duplex RT-cLAMP assay, despite demonstrating reduced detection sensitivity, has proven effective and holds promise as a supplementary approach, especially in settings with limited resources where rapid and affordable testing is crucial., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Although seagrass meadows are intense carbon sinks, information on the regional variability in seagrass blue carbon stocks and carbon sequestration remains limited. We estimated the organic carbon (C org ) stocks and carbon accumulation rates (CAR) of seven seagrass meadows along the subtropical coast of China's Zhanjiang City and analyzed the driving factors of variability in sediment C org stocks in three seagrass meadows. Results showed that most C org (99.83 %) was stored in the sediments, and the contribution of living biomass was minor. The average C org stocks of living biomass and sediments across all sites were 0.04 ± 0.01 and 42.03 ± 25.07 Mg C ha -1 , respectively, which were significantly lower than the world average (2.52 ± 0.48 and 194.2 Mg C ha -1 ). The sediment C org stocks of the upper 1 m ranged from 24.26 to 157.12 Mg C ha -1 with substantial variability among sites: Liusha Bay (64.93 ± 22.31 Mg C ha -1 ) > Donghai Island (33.8 ± 10.65 Mg C ha -1 ) > Dongshen Ferry (27.35 ± 4.15 Mg C ha -1 ). The average sediment CAR was 53.47 g C m -2  yr -1 , and the total CAR of 864.18 ha seagrass meadows was 260.76 ± 4.86 Mg C yr -1 in these studied sites. Physicochemical factors, such as high moisture content, salinity, CaCO 3 content, and low dry bulk density, jointly inhibited the mineralization rate of C org in sediments. Our study provides data from understudied regions to a growing dataset on seagrass carbon stocks and sequestration rates and highlights the significance of local and regional differences in seagrass blue carbon storage to accurately assess the climate change mitigation potential of seagrass ecosystems., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Xiaomei Shen reports financial support was provided by Guangdong Provincial Key Laboratory of Water Quality Improvement and Ecological Restoration for Watersheds, School of Ecology, Environment and Resources, Guangdong University of Technology, Guangzhou, 510,006, China. All of the authors declare that they have no conflicts of interest. This manuscript is original research, has not been previously published, and has not been submitted for publication elsewhere. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

The impact of atmospheric pollution on the growth of European forest tree species, particularly European beech, Silver fir and Norway spruce, is examined in five mesic forests in the Czech Republic. Analyzing of basal area increment (BAI) patterns using linear mixed effect models reveals a complex interplay between atmospheric nitrogen (N) and sulphur (S) deposition, climatic variables and changing CO 2 concentrations. Beech BAI responds positively to N deposition (in tandem with air CO 2 concentration), with soil phosphorus (P) availability emerging as a significant factor influencing overall growth rates. Fir BAI, on the other hand, was particularly negatively influenced by S deposition, although recent growth acceleration suggests growth resilience in post-pollution period. This fir growth surge likely coincides with stimulation of P acquisition following the decline of acidic pollution. The consequence is the current highest productivity among the studied tree species. The growth dynamics of both conifers were closely linked to the stoichiometric imbalance of phosphorus in needles, indicating the possible sensitivity of exogenous controls on nutrient uptake. Furthermore, spruce BAI was positively linked to calcium availability across sites. Despite enhanced water-use efficiency under elevated CO 2 , spruce growth is constrained by precipitation deficit and demonstrates weakening resilience to increasing growing season air temperatures. Overall, these findings underscore the intricate relationships between atmospheric pollution, nutrient availability, and climatic factors in shaping the growth dynamics of European forest ecosystems. Thus, incorporating biogeochemical context of nutrient availability is essential for realistic modelling of tree growth in a changing climate., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)