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The delimitation of nodal regions belongs among the fundamental methods of studying spatial organisation. Nodal regions are defined as regions in which intraregional interactions are closed. With respect to the available information on spatial interactions, nodal regions are defined especially on the basis of data on commuting to work, migration flows, etc. Much less attention, however, has been paid to the theoretical and methodological questions of delimitation of nodal regions based on transport flows. In this case, transport flows that rank among the bearers of basic spatial interactions have high explanatory power, as they reflect not only commuting relations (commuting to work), but also service, business, recreational and other relations. The goal of this paper is to delimit nodal regions based on car transport flows in two regions in the Czech Republic and to evaluate their relevance in studying complex socio-economic relations within the regions. [ABSTRACT FROM AUTHOR]