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Start Over Topic electrospray ionization mass spectrometry Topic liquid chromatography Publication Year Range Last 10 years Journal journal of separation science

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1. Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time‐of‐flight‐mass spectrometry.

Author

Sadowski, Radosław, Gadzała‐Kopciuch, Renata, and Buszewski, Bogusław

Subjects
*QUALITATIVE chemical analysis, *ELECTROSPRAY ionization mass spectrometry, *MOLECULAR weights, *QUADRUPOLES, *SPECTROMETRY, *OLIGOSACCHARIDE analysis
Abstract

Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3‐O‐sulfo group‐containing glucosamine residues at their reducing ends. Because these tetrasaccharides are derived from antithrombin III‐binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin. Therefore, this paper presents a new low molecular weight heparin sample preparation method–chemical depolymerization. Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far. Qualitative on‐line liquid chromatography–mass spectrometric analysis of these resistant oligosaccharides is also described in this paper. [ABSTRACT FROM AUTHOR]

Published
2020
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