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Abstract The pulp and paper industry generates vast quantities of paper sludge, posing significant environmental challenges due to its disposal in landfills or incineration. This study explores the potential of valorizing paper sludge by incorporating it into particleboard production. It aims to optimize sludge content and particle size to enhance board properties—a novel approach to waste management in the wood composites industry. Through systematic variation of sludge content (0–25%) and particle size ( 2 mm), we assessed the mechanical and physical properties such as internal bond strength (IB), modulus of rupture (MOR), modulus of elasticity (MOE), water absorption (WA), and thickness swelling (TS). The findings indicate that incorporating paper sludge at moderate levels (5–15%) with optimized particle sizes (

Abstract Nucleic acid amplification testing has great potential for point-of-need diagnostic testing with high detection sensitivity and specificity. Current sample preparation is limited by a tedious workflow requiring multiple steps, reagents and instrumentation, hampering nucleic acid testing at point of need. In this study, we present the use of mixed cellulose ester (MCE) paper for DNA binding by ionic interaction under molecular crowding conditions and fluid transport by wicking. The poly(ethylene) glycol-based (PEG) reagent simultaneously provides the high pH for alkaline lysis and crowding effects for ionic binding of the DNA under high salt conditions. In this study, we introduce Paper-based Abridged Solid-Phase Extraction with Alkaline Poly(ethylene) Glycol Lysis (PASAP). The anionic mixed cellulose ester (MCE) paper is used as solid phase and allows for fluid transport by wicking, eliminating the need for pipetting skills and the use of a magnet to retain beads. Following the release of DNA from the cells due to the lytic activity of the PASAP solution, the DNA binds to the anionic surface of the MCE paper, concentrating at the bottom while the sample matrix is transported towards the top by wicking. The paper was washed by dipping it in 40% isopropanol for 10 s. After air-drying for 30 s, the bottom section of the paper (3 mm × 4 mm) was snapped off using the cap of a PCR tube and immersed in the colourimetric loop-mediated isothermal amplification (cLAMP) solution for direct amplification and colourimetric detection. The total sample processing was completed in 15 min and ready for amplification. cLAMP enabled the detection of 102 CFU/mL of Escherichia coli (E. coli) from culture media and the detection of E. coli in milk

Abstract The paper industry is a major environmental polluter due to paper waste sludge (PWS), often disposed of in hazardous ways. The techniques are employed to disposing of PWS are posing significant environmental hazards and risks to well-being. This study aims to evaluate PWS as a potential replacement for commercial adsorbents like AC and ZEO in treating stabilized leachate. Contact angle analysis of PWS was 92.60°, reveals that PWS to be hydrophobic. Batch adsorption experiments were conducted with parameters set at 200 rpm stirring speed, 120 min contact time, and pH 7. Optimal conditions for COD and NH3–N removal were identified at 120 min contact time, 200 rpm stirring speed, pH 7, and 2.0 g PWS ratio. Removal percentages for COD and NH3–N were 62% and 52%, respectively. Based on the results of the isotherm and kinetic studies, it was observed that the Langmuir and Pseudo second order (PSO) model exhibited greater suitability compared to the Freundlich and Pseudo first order (PFO) model, as indicated by higher values of R-squared (R2). The R-squared of Langmuir for COD and NH3–N were 0.9949 and 0.9919 and for Freundlich model were 0.9855 and 0.9828 respectively. Whereas the R-squared of PFO for COD and NH3–N were 0.9875 and 0.8883 and for PSO were 0.9987 and 0.9909 respectively.

This research was designed to assess the yield and quality of ash prepared from different types of waste papers. Waste newspapers, writing papers, and cartons were selected for combustion to obtain the ashes and coded as WNPA, WWPA, and WCPA, respectively. The waste papers were incinerated simultaneously in separate but identical incinerators at 850 ℃. An experiment on the yield of the ashes was performed on two days before the ashes obtained were mixed based on the type of waste papers from which they were derived. The results showed that irrespective of the day and time, the WCPA was the most yielded (14.1%), followed by the WWPA (12.3%), and then the WNPA (11.9%). Also, it was found that all the ashes possessed flow properties acceptable for engineering applications. However, the WCPA would ensure the best performance if applied, especially as a partial replacement material for cement. While the WWPA was found to be richer than the WNPA, the WCPA was the richest in terms of the proportions of key oxides for strength development. In general, the percentages of various chemical components of each ash complied with the standard specifications for cement except in the case of losses on ignition of the WNPA and WWPA, which were about 0.53% and 0.02%, respectively, beyond the maximum stipulated value.

Abstract Laser‐induced graphene (LIG) technology has provided a new manufacturing strategy for the rapid and scalable assembling of triboelectric nanogenerators (TENG). However, current LIG‐based TENG commonly rely on polymer films, e.g., polyimide (PI) as both friction material and carbon precursor of electrodes, which limit the structural diversity and performance escalation due to its incapability of folding and creasing. Using specialized PI paper composed of randomly distributed PI fibers to substantially enhance its foldability, this work creates a new type of TENG, which are structurally foldable and stackable, and performance tailorable. First, by systematically investigating the laser power‐regulated performance of single‐unit TENG, the open‐circuit voltage can be effectively improved. By further exploiting the folding process, multiple TENG units can be assembled together to form multi‐layered structures to continuously expand the open‐circuit voltage from 5.3 to 34.4 V cm−2, as the increase of friction units from 1 to 16. Last, by fully utilizing the unique structure and performance, representative energy‐harvesting and smart‐sensing applications are demonstrated, including a smart shoe to recognize running motions and power LEDs, a smart leaf to power a thermometer by wind, a matrix sensor to recognize writing trajectories, as well as a smart glove to recognize different objects.

Large amounts of waste paper are generated annually worldwide. Although some of it is recycled, up to 50% is landfilled or incinerated. The remanufacturing of waste paper to produce pencils is proposed as a novel, sustainable business solution. A sustainability analysis of this process was performed to quantify indexes of technical, environmental, economic, and social sustainability. Small-to-medium business models were evaluated, in which 15 000 pencils/shift/day can be produced from 135 kg of waste paper, with a maximum productivity of 64 800 pencils/day. Productivity, operating costs, power consumption, land footprint, machine delivery cost, and number of workers were used to analyze the technical feasibility. The cost-to-profit ratio, cost and profit per pencil, and daily profit were used to evaluate economic sustainability. The amounts of municipal solid waste and recovered paper waste, saved embodied energy, and prevented CO2 emissions were used to analyze environmental sustainability. The number of workers and labor costs were used to evaluate human development and social sustainability. The machines required for the remanufacturing line are considered sufficiently mature, remanufactured pencils are less expensive to produce than wooden pencils, and the proposed process minimizes the amount of waste paper sent to landfills and avoids the use of new wood for producing pencils, thereby satisfying technical, economic, and environmental sustainability, respectively. The final sustainability index of 0.9 is considered very high and sufficient for operating a profitable, sustainable business with a profit of 252–583 USD/day.

At present, blockchain technology has been applied in different fields such as finance, Internet of Things, logistics, digital copyright, and public services, but it is less used in the field of paper-cut animation. Therefore, the use of blockchain to design a copyright protection model for paper-cut animation design based on blockchain is crucial to the progress of paper-cut animation design. This work combines various technologies such as blockchain technology, InterPlanetary File System (IPFS) technology, and Node.js technology for integrated design and construction. In the construction of the system model, the relevant characteristics of the blockchain technology show that the blockchain can provide a new and effective method for digital copyright protection; IPFS technology provides distributed and secure storage for massive amounts of oil and gas knowledge and achievement data; and the relevant development framework and technical characteristics of Node.js technology provide a reliable programming foundation for the realization of the system. Through the construction of the digital copyright protection system model based on the blockchain, the blockchain technology is used to realize the protection of the digital copyright of the knowledge achievement data of paper-cut animation. The results show that the model has high security and is more economical and practical than other existing models. In a word, the copyright protection model of paper-cut animation design based on blockchain can fully protect the rights and interests of creative talents of paper-cut animation, thereby promoting the progress of paper-cut animation industry.

Abstract Mechanochromic photonic crystals are attractive due to their force-dependent structural colors; however, showing unrecordable color and unsatisfied performances, which significantly limits their development and expansion toward advanced applications. Here, a thermal-responsive mechanochromic photonic crystal with a multicolor recordability-erasability was fabricated by combining non-close-packing mechanochromic photonic crystals and phase-change materials. Multicolor recordability is realized by pressing thermal-responsive mechanochromic photonic crystals to obtain target colors over the phase-change temperature followed by fixing the target colors and deformed configuration at room temperature. The stable recorded color can be erased and reconfigured by simply heating and similar color-recording procedures respectively due to the thermoswitchable on-off mechanochromism of thermal-responsive mechanochromic photonic crystals along with solid-gel phase transition. These thermal-responsive mechanochromic photonic crystals are ideal rewritable papers for ink-freely achieving multicolor patterns with high resolution, difficult for conventional photonic papers. This work offers a perspective for designing color-recordable/erasable and other stimulus-switchable materials with advanced applications.

Since the last two decades, the use of natural fiber reinforced polymer composites has garnered significant application considerations over the synthetic fiber reinforced composites due to their numerous advantages and unique properties. Likewise, epoxy resin has attracted the interest of many researchers for composite synthesis, basically because of its chemical stability, thermal and mechanical characteristics. Hence, the primary aim of this study was to investigate the influence of dombeya fiber and paper particulate on the physical and mechanical properties of dombeya fiber and paper particulate-reinforced polymer composites for structural applications. Dombeya fiber and paper particles are renewable and biodegradable materials, thereby making them environmentally friendly materials to replace synthetic materials. Hand lay-up technique was utilized to fabricate the hybrid-reinforced biocomposites after which they were subjected to mechanical, wear, density, and moisture absorption properties. The surface morphology of the fractured surface was also analyzed to investigate its microstructural features. It was discovered from the results that hybrid biocomposites demonstrated improved properties over the unreinforced composite, with composites from 9-wt% dombeya fiber-paper particles reinforced biocomposite exhibiting the most suitable properties with commensurate density with the unreinforced epoxy matrix. These obtained characteristics support the material as a suitable material for biomedical apparatus application such as orthopedic implants, surgical instruments, and bone fixation devices.

Abstract Personalized healthcare management is an emerging field that requires the development of environment‐friendly, integrated, and electrochemical multimodal devices. In this study, the concept of integrated paper‐based biosensors (IFP−Multi) for personalized healthcare management is introduced. By leveraging ink printing technology and a ChatGPT–bioelectronic interface, these biosensors offer ultrahigh areal‐specific capacitance (74633 mF cm−2), excellent mechanical properties, and multifunctional sensing and humidity power generation capabilities. More importantly, the IFP−Multi devices have the potential to simulate deaf‐mute vocalization and can be integrated into wearable sensors to detect muscle contractions and bending motions. Moreover, they also enable monitoring of physiological signals from various body parts, such as the throat, nape, elbow, wrist, and knee, and successfully record sharp and repeatable signals generated by muscle contractions. In addition, the IFP−Multi devices demonstrate self‐powered handwriting sensing and moisture power generation for sweat‐sensing applications. As a proof‐of‐concept, a GPT 3.5 model‐based fine‐tuning and prediction pipeline that utilizes recorded physiological signals through IFP−Multi is showcased, enabling artificial intelligence with multimodal sensing capabilities for personalized healthcare management. This work presents a promising and ecofriendly approach to developing paper‐based electrochemical multimodal devices, paving the way for a new era of healthcare advancements.

Abstract Microfluidic paper-based analytical devices often are combined with scanners as detectors. In this work, different scanning options offered by scanners: resolution, scanning mode, exposure to radiation, colour restoration, and saving format were tested. Moreover, different attempts to mathematical data treatment based on intensities of three channels—Red, Green and Blue, were studied. All measurements presented in this article were conducted for a model dye—bromothymol blue and a model analyte—zinc(II) ion (complexed with xylenol orange in a paper matrix). The article summarizes the scanning options and possibilities of mathematical calculations. Nevertheless, it is suggested that the best option is to use the prior prepared calculation file to paste obtained intensities and compare all presented in this article (and the most frequently used) equations to process intensities and decide which one should be used in the particular analysis.

Abstract Anger suppression is important in our daily life, as its failure can sometimes lead to the breaking down of relationships in families. Thus, effective strategies to suppress or neutralise anger have been examined. This study shows that physical disposal of a piece of paper containing one’s written thoughts on the cause of a provocative event neutralises anger, while holding the paper did not. In this study, participants wrote brief opinions about social problems and received a handwritten, insulting comment consisting of low evaluations about their composition from a confederate. Then, the participants wrote the cause and their thoughts about the provocative event. Half of the participants (disposal group) disposed of the paper in the trash can (Experiment 1) or in the shredder (Experiment 2), while the other half (retention group) kept it in a file on the desk. All the participants showed an increased subjective rating of anger after receiving the insulting feedback. However, the subjective anger for the disposal group decreased as low as the baseline period, while that of the retention group was still higher than that in the baseline period in both experiments. We propose this method as a powerful and simple way to eliminate anger.

Abstract The erratic, intermittent, and unpredictable nature of sweat production, resulting from physiological or psychological fluctuations, poses intricacies to consistently and accurately sample and evaluate sweat biomarkers. Skin‐interfaced microfluidic devices that rely on colorimetric mechanisms for semi‐quantitative detection are particularly susceptible to these inaccuracies due to variations in sweat secretion rate or instantaneous volume. This work introduces a skin‐interfaced colorimetric bifluidic sweat device with two synchronous channels to quantify sweat rate and biomarkers in real‐time, even during uncertain sweat activities. In the proposed bifluidic‐distance metric approach, with one channel to measure sweat rate and quantify collected sweat volume, the other channel can provide an accurate analysis of the biomarkers based on the collected sweat volume. The closed channel design also reduces evaporation and resists contamination from the external environment. The feasibility of the device is highlighted in a proof‐of‐the‐concept demonstration to analyze sweat chloride for evaluating hydration status and sweat glucose for assessing glucose levels. The low‐cost yet highly accurate device provides opportunities for clinical sweat analysis and disease screening in remote and low‐resource settings. The developed device platform can be facilely adapted for the other biomarkers when corresponding colorimetric reagents are exploited.

The exponential increase in the number of submissions, further accelerated by generative AI, and the decline in the availability of experts are burdening the peer review process. This has led to high unethical desk rejection rates, a growing appeal for the publication of unreviewed preprints, and a worrying proliferation of predatory journals. The idea of monetarily compensating peer reviewers has been around for many years; maybe, it is time to take it seriously as one way to save the peer review process. Here, I argue that paying reviewers, when done in a fair and transparent way, is a viable solution. Like the case of professional language editors, part-time or full-time professional reviewers, managed by universities or for-profit companies, can be an integral part of modern peer review. Being a professional reviewer could be financially attractive to retired senior researchers and to researchers who enjoy evaluating papers but are not motivated to do so for free. Moreover, not all produced research needs to go through peer review, and thus persuading researchers to limit submissions to their most novel and useful research could also help bring submission volumes to manageable levels. Overall, this paper reckons that the problem is not the peer review process per se but rather its function within an academic ecosystem dominated by an unhealthy culture of ‘publish or perish’. Instead of reforming the peer review process, academia has to look for better science dissemination schemes that promote collaboration over competition, engagement over judgement, and research quality and sustainability over quantity.

The exponential increase in the number of submissions, further accelerated by generative AI, and the decline in the availability of experts are burdening the peer review process. This has led to high unethical desk rejection rates, a growing appeal for the publication of unreviewed preprints, and a worrying proliferation of predatory journals. The idea of monetarily compensating peer reviewers has been around for many years; maybe, it is time to take it seriously as one way to save the peer review process. Here, I argue that paying reviewers, when done in a fair and transparent way, is a viable solution. Like the case of professional language editors, part-time or full-time professional reviewers, managed by universities or for-profit companies, can be an integral part of modern peer review. Being a professional reviewer could be financially attractive to retired senior researchers and to researchers who enjoy evaluating papers but are not motivated to do so for free. Moreover, not all produced research needs to go through peer review, and thus persuading researchers to limit submissions to their most novel and useful research could also help bring submission volumes to manageable levels. Overall, this paper reckons that the problem is not the peer review process per se but rather its function within an academic ecosystem dominated by an unhealthy culture of ‘publish or perish’. Instead of reforming the peer review process, academia has to look for better science dissemination schemes that promote collaboration over competition, engagement over judgement, and research quality and sustainability over quantity.

ABSTRACTThe effect of bamboo virgin pulp addition to the recycled pulp in enhancing the recycled paper’s mechanical properties was evaluated. Malaysian bamboo virgin pulp used in this research were Bambusa vulgaris (Aur bamboo), Gigantochloa levis (Beting bamboo), and Gigantochloa scortechinii (Semantan bamboo). The addition of 2.5, 5, 7.5, and 10 wt% of bamboo virgin pulp improved the burst index, folding endurance, tearing index, and tensile index of the recycled paper from 2.27 to 3.17 kPa.m2/g, 7 to 15 double folds, 5.98 to 8.27 mN.m2/g, and 24.86 to 32.40 Nm/g, respectively. These were equivalent to the increment percentage for burst index, folding endurance, tearing index, and tensile index at the range of 6.61 to 39.65%, 28.57 to 114.29%, 15.72 to 38.59%, and 10.50 to 33.87%, respectively. The application of bamboo virgin pulp is very promising in improving the recycled paper’s mechanical properties and this could be further extended in enhancing the other properties such as physical and optical paper properties.

Abstract The research presented in this paper introduces a novel environmental energy‐harvesting technology that harnesses electricity from the evaporation of water using porous structural materials. Specifically, a strategy employing paper‐based hydroelectric generators (p‐HEGs) is proposed to capture the energy produced during water evaporation and convert it into usable electricity. The p‐HEGs offer several advantages, including simplicity in fabrication, low cost, and reusability. To evaluate their effectiveness, the water evaporation‐induced electrical output performance of four different p‐HEGs are compared. Among the variants tested, the p‐HEG combining wood pulp and polyester fiber exhibits the best output performance. At room temperature, this particular p‐HEG generates a short‐circuit current and open‐circuit voltage of ≈0.4 µA and 0.3 V, respectively, thereby demonstrating excellent electrical stability. Furthermore, the electrical current and voltage generated by the p‐HEG through water evaporation are able to power an LED light, both individually and in series and parallel connections. This study delves into the potential of electricity harvesting from water evaporation and establishes it as a viable method for renewable energy applications.

Cellulose paper-based sensing devices have shown promise in addressing the accuracy, sensitivity, selectivity, analysis time and cost of current disease diagnostic tools owing to their excellent physical and physiochemical properties, high surface-area-to-volume ratio, strong adsorption capabilities, ease of chemical functionalization for immobilization, biodegradability, biocompatibility and liquid transport by simple capillary action. This review provides a comprehensive overview of recent advancements in the field of electrochemical immunosensing for various diseases, particularly in underdeveloped regions and globally. It highlights the significant progress in fabrication techniques, fluid control, signal transduction and paper substrates, shedding light on their respective advantages and disadvantages. The primary objective of this review article is to compile recent advances in the field of electrochemical immunosensing for the early detection of diseases prevalent in underdeveloped regions and globally, including cancer biomarkers, bacteria, proteins and viruses. Herein, the critical need for new, simplistic early detection strategies to combat future disease outbreaks and prevent global pandemics is addressed. Moreover, recent advancements in fabrication techniques, including lithography, printing and electrodeposition as well as device orientation, substrate type and electrode modification, have highlighted their potential for enhancing sensitivity and accuracy.

Abstract We report an innovative and facile approach to fabricating an ultrasensitive plasmonic paper substrate for surface-enhanced Raman spectroscopy (SERS). The approach exploits the self-assembling capability of poly(styrene-b-2-vinyl pyridine) block copolymers to form a thin film at the air-liquid interface within the single microdroplet scale for the first time and the subsequent in situ growth of silver nanoparticles (AgNPs). The concentration of the block copolymer was found to play an essential role in stabilizing the droplets during the mass transfer phase and formation of silver nanoparticles, thus influencing the SERS signals. SEM analysis of the morphology of the plasmonic paper substrates revealed the formation of spherical AgNPs evenly distributed across the surface of the formed copolymer film with a size distribution of 47.5 nm. The resultant enhancement factor was calculated to be 1.2 × 107, and the detection limit of rhodamine 6G was as low as 48.9 pM. The nanohybridized plasmonic paper was successfully applied to detect two emerging pollutants—sildenafil and flibanserin—with LODs as low as 1.48 nM and 3.45 nM, respectively. Thus, this study offers new prospects for designing an affordable and readily available, yet highly sensitive, paper-based SERS substrate with the potential for development as a lab-on-a-chip device.

Abstract There has been a growing effort to replace manual extraction of data from research papers with automated data extraction based on natural language processing, language models, and recently, large language models (LLMs). Although these methods enable efficient extraction of data from large sets of research papers, they require a significant amount of up-front effort, expertise, and coding. In this work, we propose the ChatExtract method that can fully automate very accurate data extraction with minimal initial effort and background, using an advanced conversational LLM. ChatExtract consists of a set of engineered prompts applied to a conversational LLM that both identify sentences with data, extract that data, and assure the data’s correctness through a series of follow-up questions. These follow-up questions largely overcome known issues with LLMs providing factually inaccurate responses. ChatExtract can be applied with any conversational LLMs and yields very high quality data extraction. In tests on materials data, we find precision and recall both close to 90% from the best conversational LLMs, like GPT-4. We demonstrate that the exceptional performance is enabled by the information retention in a conversational model combined with purposeful redundancy and introducing uncertainty through follow-up prompts. These results suggest that approaches similar to ChatExtract, due to their simplicity, transferability, and accuracy are likely to become powerful tools for data extraction in the near future. Finally, databases for critical cooling rates of metallic glasses and yield strengths of high entropy alloys are developed using ChatExtract.

Abstract Energy storage devices (ESD) which are intended to power electronic devices, used in close contact of human skin, are desirable to be safe and non-toxic. In light of this requirement, Zn based energy storage devices seem to provide a viable pathway as they mostly employ aqueous based electrolytes which are safe and non-toxic in their functioning. Additionally, having a flexible ESD will play a crucial role as it will enable the ESD to conform to the varying shapes and sizes of wearable electronics which they energize. In this work, we have developed an inkjet-printed Zinc ion battery (IPZIB) with planar electrode configuration over bond paper substrate. Zn has been used as the negative electrode, MnO2 is used as the positive electrode with Poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) as the active binder. Conducting tracks of reduced graphene oxide (rGO) are used to construct the current collector on the paper substrate. The fabricated IPZIB delivered a high discharge capacity of 300.14 mAh g−1 at a current density of 200 mA g−1. The energy density of the IPZIB is observed as 330.15 Wh kg−1 at a power density of 220 W kg−1 and retains an energy density of 94.36 Wh kg−1 at a high power density of 1650 W kg−1. Finally, we have demonstrated the capability of the IPZIB to power a LED at various bending and folding conditions which indicates its potential to be used in the next generation flexible and wearable electronic devices.

When deciding which submissions should be peer reviewed, eLife editors consider whether they will be able to find high-quality reviewers, and whether the reviews will be valuable to the scientific community.

Abstract Oil-immersed transformers are expensive equipment in the electrical system, and their failure would lead to widespread blackouts and catastrophic economic losses. In this work, an elaborate diagnostic approach is proposed to evaluate twenty-six different transformers in-service to determine their operative status as per the IEC 60599:2022 standard and CIGRE brochure. The approach integrates dissolved gas analysis (DGA), transformer oil integrity analysis, visual inspections, and two Back Propagation Neural Network (BPNN) algorithms to predict the loss of life (LOL) of the transformers through condition monitoring of the cellulose paper. The first BPNN algorithm proposed is based on forecasting the degree of polymerization (DP) using 2-Furaldehyde (2FAL) concentration measured from oil samples using DGA, and the second BPNN algorithm proposed is based on forecasting transformer LOL using the 2FAL and DP data obtained from the first BPNN algorithm. The first algorithm produced a correlation coefficient of 0.970 when the DP was predicted using the 2FAL measured in oil and the second algorithm produced a correlation coefficient of 0.999 when the LOL was predicted using the 2FAL and DP output data obtained from the first algorithm. The results show that the BPNN can be utilized to forecast the DP and LOL of transformers in-service. Lastly, the results are used for hazard analysis and lifespan prediction based on the health index (HI) for each transformer to predict the expected years of service.

Abstract As the social organism, Polistes wasps build a communal nest using woody fibers with saliva for sustaining brood and adult population throughout the season. Limited information exists regarding the identification specific plant materials employed in wasp nest building. Thus, we firstly tested if the DNA metabarcoding approach utilizing rbcL and trnL molecular markers could identify the plant species quantitatively and qualitatively inform the mixed-origin woody samples. A threshold of 0.01 proportion of reads was applied for rbcL and trnL molecular markers, while this threshold for median proportion was 0.0025. In assessing taxa richness, the median proportion demonstrated superior performance, exhibiting higher taxa detection power, however, rbcL marker outperformed in quantitative analysis. Subsequently, we applied DNA metabarcoding to identify the plant materials from the nests of two Polistes species, P. mandarinus and P. rothneyi. The results showed that higher preference of Quercus and Robinia as the major nest building materials regardless of the surrounding plant communities, by two wasp species. Material diversity was higher for P. rothneyi than P. mandarinus, which may explain the abundance of this species possibly with heightened adaptive capacities in their nesting behavior. This study demonstrated that DNA metabarcoding could identify the complex nest-building plant materials of paper wasps and provide insights into their ecological interactions in the natural ecosystem.

Abstract Pronoun usage’s psychological underpinning and behavioral consequence have fascinated researchers, with much research attention paid to second-person pronouns like “you,” “your,” and “yours.” While these pronouns’ effects are understood in many contexts, their role in bilateral, dynamic conversations (especially those outside of close relationships) remains less explored. This research attempts to bridge this gap by examining 25,679 instances of peer review correspondence with Nature Communications using the difference-in-differences method. Here we show that authors addressing reviewers using second-person pronouns receive fewer questions, shorter responses, and more positive feedback. Further analyses suggest that this shift in the review process occurs because “you” (vs. non-“you”) usage creates a more personal and engaging conversation. Employing the peer review process of scientific papers as a backdrop, this research reveals the behavioral and psychological effects that second-person pronouns have in interactive written communications.

Abstract Traditionally, cellulose nanofiber (CNF) production has primarily relied on virgin cellulose sources. Yet, the shift to using paper mill sludge (PMS) as a source for CNF underscores the significance of reusing and recycling industrial byproducts. PMS contains significant amounts of cellulose that can be extracted as a raw material. The purpose of present study is to provide a sustainable approach to PMS utilization as a paper coating additive in the cellulose nanofibrils (CNFPMS) form via simply scalable wire-wound rod coating method. The effect of CNFPMS additive amounts at two coating layers on microstructure and surface properties of coatings such as porosity, air permeability surface roughness and optical properties such as brightness, gloss and CIE L*a*b* is studied, which they can also provide insight for the eventual print performance. Results indicated that the obtained CNFPMS in paper coating shows 52% decrease in porosity, presenting significant improvement in the coating microstructure. The marginal increase in permeability coefficient and surface roughness, 54% and 10%, respectively, suggests improving color reproduction and preventing color density losses. Optical analysis showed slight decrease in brightness and gloss, as was expected. Notably, the lightness was improved, which also indicates increasing color gamut volume in printing applications. As a result, the current work offers a sustainable approach to manage PMS for use in paper coatings as a high-value-added material.

Strength properties of paper are of great significance in terms of its mechanical performance and are attributed to cellulosic fibers. When filler is used in papermaking, the cost and strength properties get reduced but optical properties are improved. So far, modification of fillers using chitosan (CH) is not explored in such a way that all aspects of wet-end of papermaking get covered. The novelty lies in showing the effect of CH as filler modifier at various doses on different fillers and the wet-end application of modified fillers at different doses along with other additives used in papermaking. The impact of addition of modified fillers on end paper properties is also analyzed. Here, CH was applied to modify fillers characteristics so that the ash in paper can be increased without compromising the strength properties. Three different doses (0.25%, 0.50% and 1.00%) of CH were added to the fillers. The addition of modified fillers to bleached mixed hardwood (BMHW) pulp increased the filler retention in paper while the reduction in strength properties was found to be less and in some properties even an increase was observed. The particle size distributions of fillers were found to be affected after modification of fillers.

Abstract Cellulose paper has emerged as an ideal sensing element for wearable pressure sensors owing to its inherent flexibility, high porosity, and light weight. However, traditional paper‐based pressure sensors use metal‐based materials as electrodes, which significantly limits the unique advantages of paper, particularly in terms of degradability. In this study, a degradable pressure sensor is designed by combining the highly conductive poly(3,4‐ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) Xuan paper electrode with a low‐conductivity PEDOT:PSS tissue paper sensitive layer. Notably, Xuan paper, also called rice paper, has been a prominent substrate owing to its softness and good durability. By introducing a perforated structure in the sensitive layer, a novel sensing mechanism, conductivity conversion under pressure, is realized to improve the sensitivity. The obtained sensor exhibits a high sensitivity (13.9 kPa−1 at < 8.3 kPa, 151 kPa−1 at 8.3–20.8 kPa), ensuring that it can precisely monitor the full‐range human activities. Additionally, as the sensor does not rely on metal materials, it can degrade in water or fire without causing any negative environmental impacts. These findings establish a new approach to producing highly sensitive degradable sensors, which hold significant potential for application in green electronics, paper‐based sensing matrices, new prosthetics, and other fields.

In this study, hemp-based barrier papers were produced by a facile film coating route. The hemp plant was mechanically separated by crushing and breaking down the stalks. The woody hurd and fibers were separated manually. Then, cellulosic pulp was prepared by the Kraft method. Alkali treated hemp fibers were used for the paper preparation, and papers were coated with poly(lactic acid) (PLA) films. Physical, morphological, structural, wetting, mechanical properties, thermal dimensional stability, and water vapor permeability (WVP) properties were analyzed. Average hemp fiber diameter decreased by around 50% after alkali treatment. It was also found that dry and wet tensile strength values for PLA-coated hemp papers increased 3- and 2-orders of magnitudes, respectively. Also, PLA-coated hemp papers showed 0% shrinkage in the temperature range from 100°C to 200°C. WVP of PLA-coated hemp papers decreased by around 50% compared to hemp paper at the end of 300 h.

Abstract This article presents the results of an experiment designed to study the impact of temperature on the characteristic parameters of creeping discharges. The insulating interfaces consist of a thermally enhanced cellulose surface immersed in mineral oil, palm kernel oil methyl ester (PKOME) and castor oil methyl ester (COME). The study was carried out under a standard negative lightning impulse voltage (1.2/50 μs). The article also presents the complete algorithms for calculating the maximum extension of the discharges, the ionisation rate and the charge produced by them. The results of the study show that temperature favors the propagation of discharges and the ionisation rate. It was observed that liquids with a higher dielectric constant and high electrical conductivity were more exposed to the impact of temperature. The results show ionisation increments of 0.973%/°C, 1.093%/°C and 1.076%/°C in mineral oil (MO), COME and PKOME respectively. The maximum extension of the discharges shows a linear evolution with the applied voltage and temperature but a non-linear increment with the temperature. As for the charge produced, it shows a constant increment with temperature and voltage in each liquid. These values are (5.839%/°C, 1.977%/kV), (6.047%/°C, 2.082%/kV) and (6.177%/°C, 2.113%/kV) respectively in MO, COME and PKOME.

Hypothesis Descriptions are a type of manuscript dedicated to the formal description of a hypothesis, as introduced in an accompanying editorial and an examplary Hypothesis Description for the Enemy Release Hypothesis that is used in invasion biology. This questionnaire provides a template for such a Hypothesis Description manuscript. The template's format was designed for simplicity to facilitate adoption, and it can be easily extended to capture additional information, e.g. instructions for falsification or generalization, taxonomic or geographic scope, etymology, or relevant information in other research fields or other languages. The template reflects the recommended structure for a Hypothesis Description manuscript in that each of its sections provides the title for a section in a Hypothesis Description manuscript and indicates whether that section is mandatory or optional. Four sections - Keywords (mandatory), Conflicts of interest (optional), Acknowledgments (optional) and References (mandatory) - are in this template filled in for the template itself but should otherwise be adjusted for the hypothesis at hand. Comments to guide authors who work on a Hypothesis Description manuscript are provided as well.

Waste papers, which are a major constituent of municipal wastes, can be repurposed into the production of bioethanol, a renewable and ecofriendly fuel. Many studies have produced ethanol from print-grade papers, but literature on conversion of waste cartons is sparce. In this study, waste corrugated cartons and print-grade papers were reduced into slurries and were subjected to physical and chemical tests to determine their suitability for biosynthesis. Cellulose fibers in the slurries were directly hydrolyzed into glucose without prior pretreatment by dilute hydrochloric acid, and fermented into ethanol using Saccaromyces cerevisae. Density, flammability, color, odor, non-volatile residue and other qualitative tests were conducted on the ethanol produced. Glucose yield and the extraction efficiency in cellulose hydrolysis were significantly higher for waste cartons than print paper slurries. Qualitative tests reveal that the ethanol produced from both slurries has physical and chemical characteristics that are comparable to laboratory grade ethanol, contains no toxic impurities, and is suitable for use as a biofuel.

Basalt fiber is a famous sound and thermal insulating material. In this study, a novel basalt fiber-based electrical insulating paper was successfully prepared, and its physical strength, dielectric properties, thermal stability, and glue permeability of the composite paper were systematically investigated. The results showed that the introduction of mica could make basalt fiber/mica composite paper (B-M paper). Meanwhile, the introduction of aramid fibrids into B-M paper could greatly increase the physical strength and dielectric performance (1–26 times). In addition, silicone adhesive bonding could further enhance physical strength and electrical properties, which were 1–45 times higher than those of pure composite paper. Thus, basalt fiber-based paper is a potential electrical insulating material.

The high production of paper, followed by a more significant concern with environmental and economic issues, has led to greater relevance to paper recycling and its raw uses as composite filler. Composites of polypropylene with 10, 20, and 30 wt.% of recycled office waste paper were produced by injection molding and characterized by mechanical and thermal properties. All composites presented a slight reduction of 15°C in thermal stability compared to the polymeric matrix. Moreover, as the filler amount increased, the degree of crystallinity was reduced proportionally. The mechanical test showed similar tensile strength values among neat polymer andcomposites but also showed an increase in the composite’s tensile modulus, related to the stiffness of the natural filler. The composite with a higher percentage of waste paper (30 wt.%) maintained equivalent properties as neatpolypropylene and other composites (10 and 20 wt.%). Thus, 30 wt.% composites proved to be an excellent material with less synthetic polymer percentage, keeping thermal and mechanical properties comparable to the neat PP.Furthermore, this composite does not need chemical treatment or additives harmful to the environment. It is suitable for future applications in various plastic products such as packaging and domestic utensils.

The alarming global deforestation rate has great impacts on the output of the Pulp and paper Industry since wood is the principal papermaking fiber material. Although, generally, bamboo is an alternative cellulolytic fiber source, the species and culm age have great effects on its papermaking potential. Based on the methods by the International Association of Wood Anatomists (IAWA), this work assessed the characteristics of fibers from the axial culm sections of six-month plantation-grown Oxythenantera abyssinica and Beema bamboo (which was cloned from Bambusa balcoaa) for paper production. The lengths of the fibers ranged from 1.89 to 2.39 mm while the diameters were 0.025–0.031 mm. The fiber lumen diameter and wall thickness were 0.014–0.018 mm and 0.004–0.006 mm, respectively. Fibers from the species had their runkle ratios below 1, slenderness ratios above 70 and flexibility ratios above 50. These characteristics compare well with the recommendations for fibers often used for the production of packaging, writing and printing papers. Establishment of plantations of Beema bamboo and O. abyssinica is encouraged as they will serve as good raw material base for the paper industry within six months of planting. Additionally, their planting will contribute to meeting Sustainable Development Goals 1 and 13.

The microstructure of fibrous paper plays an important role in its property investigation. In this study, an approach is proposed to extrapolate a 2D image into a virtual 3D microstructure. Five types of handsheets made of different pulps were prepared. Then, a hybrid function of two-point correlation and lineal-path function (S2&L2) and co-occurrence correlation functions (CCFs) was used in the simulated annealing reconstruction method. Thus, microstructures of two-phase fiber-pore handsheets were reconstructed using 2D scanning electron microscopy images. Finally, penetration simulations and calculations of the absolute permeability of handsheets were conducted. The statistical values of two-point correlation function (S2) and lineal-path function (L2) extracted from the reconstructed images were used to characterize the reconstruction accuracy, and the comparisons of reconstruction accuracy and time were made. The study results showed that the 3D microstructures of fibrous handsheets could be reconstructed effectively by S2&L2 and CCFs, identifying with the targets. The accuracies were around 10−5, and the reconstruction times by CCFs were shortened by 30–60% compared with S2&L2. Moreover, the visual permeability simulation results could reflect the structural difference of handsheets, according to the calculated absolute permeability. These findings provide a guidance for 3D reconstruction of natural fiber paper.

Non-wood fibers are projected as fast-growing resources and sustainable alternate materials to reduce deforestation and enhance efficiency in the pulp and paper industry. This paper addressed the paucity of data on the fiber morphological characteristics (Fiber Length, Diameter, Lumen Diameter, and Wall Thickness) and pulping properties (Slenderness Ratio, Rigidity Coefficient, Flexibility Coefficient, Runkel Ratio, Solids Factor, and Luce’s Shape Factor) for Silk Cotton, leaves and stalk of Elephant Grass and Sugarcane Bagasse found in Ghana, especially, as environmental variations may cause differences in regional data. Observed values for the studied parameters were within the suitable range for paper production and differed from those reported for other regions. Elephant Grass (stalk) and Bagasse that had higher values of Fiber Length (4101.42 ± 197.89 and 3960.20 ± 194.35 µm), Diameter (60.03 ± 1.82 and 59.85 ± 1.72 µm), Lumen Diameter (33.33 ± 1.76 and 35.97 ± 1.38 µm), Wall Thickness (13.35 ± 0.83 and 11.94 ± 0.55 µm), Slenderness Ratio (70.8 ± 4.2 and 67.2 ± 3.4), and Flexibility Coefficient (55.7 ± 2.3 and 55.7 ± 2.3) would perform better during paper production. They would exhibit good physico-mechanical properties suitable for manufacturing flexible, high tearing, elastic, dense, smooth, and well-formed papers with high tensile and bursting strengths. Silk Cotton may also produce good papers with low tearing index, well-bonded, rigid, bulky, and coarse textured.

The objective of this study was to better understand the effect of micro/nanofibrils added as reinforcement on paper handsheets, their physical-mechanical performance and barrier properties, and to determine the ideal cellulose nanofibrils (CNF) proportion to increase such paper properties. Mechanically produced CNF were added at increasing amounts (0%, 2%, 5%, 8% and 10%) to commercial Eucalyptus pulp to produce paper handsheet samples. Morphology, crystallinity, physical-mechanical and air barrier properties of the paper handsheets were evaluated. The results suggested that adding CNF has decreased the presence of empty spaces inside and on the surface of the paper handsheets by up to 61% due to the interaction between fibers and nanofibrils. The paper handsheets became denser, more compact and resistant to passage of air, as well as with greater mechanical performance with higher CNF content (10%). The bursting index is approximately 5 times higher on paper with addition of 10% of CNF compared to control paper handsheets. There were significant gains in the studied properties without any change in CNF/fibers surface charge or the use of any cationic polymer to assist the retention of nanofibrils and fibers. This study highlights the potential of CNF as additives in papermaking process, increasing its properties.

We study a three-species cyclic model whose organisms are vulnerable to contamination with an infectious disease which propagates person-to-person. We consider that individuals of one species perform a self-preservation strategy by reducing the mobility rate to minimise infection risk whenever an epidemic outbreak reaches the neighbourhood. Running stochastic simulations, we quantify the changes in spatial patterns induced by unevenness in the cyclic game introduced by the mobility restriction strategy of organisms of one out of the species. Our findings show that variations in disease virulence impact the benefits of dispersal limitation reaction, with the relative reduction of the organisms’ infection risk accentuating in surges of less contagious or deadlier diseases. The effectiveness of the mobility restriction tactic depends on the deceleration level and the fraction of infected neighbours which is considered too dangerous, thus triggering the defensive strategy. If each organism promptly reacts to the arrival of the first viral vectors in its surroundings with strict mobility reduction, contamination risk decreases significantly. Our conclusions may help biologists understand the impact of defensive strategies in ecosystems during an epidemic.

Abstract The surface modification of filters with large pore sizes for the development of low-cost gravity-driven point-of-use (POU) technologies for water disinfection can be an effective strategy to empower people to access safe water instantly, especially in low- and middle-income countries. In this study, the surface of commercial cellulose filter papers, as cheap and bio-based filters, was modified with polydopamine (PDA), polyethyleneimine (PEI) and ZnO/Ag/GO nanocomposite (ZnO/Ag/GO@PDA/PEI papers) for bacterial removal from water. PDA/PEI incorporation introduced a cationic functional layer, which can entrap negative bacteria and make a stable chemical bond with the nanocomposite. ZnO/Ag/GO exhibited promising synergistic antibacterial activities (30 times stronger than ZnO). As a result, 3 sheets of ZnO/Ag/GO@PDA/PEI papers showed a 99.98% bacterial reduction (E. coli), which met the WHO standards. Moreover, the leached zinc and silver in the filtrate were far below the WHO’s limits (380 and 10 ppb, respectively). The results showed that the modified papers could be reused multiple times. After six times of reuse, the flow rate dropped slightly (below 20%) and the bacterial removal efficiency was more than 99.9%. This study is valuable for developing filters for treating bacterial-contaminated water on-site with no need for energy, which is a demand in many countries.

Abstract Trimethylsilyl cellulose (TMSC) was employed as the coating matrix for the application of zinc oxide nanoparticles (ZnO) onto paper surfaces and the protections of ZnO/TMSC coating against UV-induced damages and fungal spoilage were evaluated. Filter papers were immersed in 2% w/v TMSC solution loaded with ZnO and air-dried. Three ZnO/TMSC suspensions were prepared with 0.1, 0.5, and 1% w/v ZnO NPs. The presences of ZnO/TMSC protective layers were confirmed with ATR-IR spectroscopy. The coated papers exhibited high surface hydrophobicities. After the coated papers were subject to 365-nm UV irradiation at 400 W for 3 h, the contact angles dramatically dropped. The trimethylsilyl (TMS) groups exposed on the surface formed a moisture barrier and were partially removed on UV exposure. ATR-IR revealed that more TMS groups were removed in the protective layer with no ZnO. UV-irradiated papers turned yellow and papers protected with 1% ZnO/TMSC exhibited significantly lower color changes than that of the uncoated one. Compared to the TMSC-coated paper, the addition of ZnO resulted in a significant reduction in tensile strength at maximum. However, after UV irradiation, significant increases in both the strain at break and strength at maximum were only observed in 1% ZnO/TMSC-protected papers. Regarding their anti-fungal properties, the 1% ZnO/TMSC films were effective in growth inhibitions of Aspergillus sp. and Penicillium sp. on the nonirradiated papers. Despite being hydrophilic after UV-irradiation, growths of the molds were severely suppressed on the UV-irradiated paper.

Increasing temperature can enhance the geographical spread and behavior of disease vector mosquitoes, exposing vulnerable populations to Aedes-borne viruses and infections. To address this risk, cost-effective and sustained intervention vector control tools are required, such as volatile pyrethroid spatial repellents. This study used a high-throughput screening system toxicity bioassay to determine the discriminating concentrations of transfluthrin-treated filter papers with variable air-drying times exposed to pyrethroid-susceptible Aedes aegypti mosquitoes. At the highest transfluthrin concentration (0.01706%), a significant reduction in mosquito mortality was observed in filter papers air-dried for 24 h compared to those air-dried for 1 h (odds ratio = 0.390, p < 0.001, 95% confidence interval: 0.23–0.66). Conversely, no significant difference in mortality was found between filter papers air-dried for 1 h and those air-dried for 12 h (odds ratio = 0.646, p = 0.107, 95% confidence interval: 0.38–1.10). The discriminating concentration was 2.8-fold higher for transfluthrin-treated filter papers air-dried for 24 h than it was for papers air-dried for 1 h, and it increased 5-fold from 1 h to 336 h of air-drying. These results show that the optimal air-drying period of transfluthrin-treated filter paper is critical, as higher discriminating concentration values may lead to underestimations of insecticide resistance. The instability of transfluthrin-treated papers necessitates the use of the World Health Organization (WHO) bottle bioassay, which is the preferred method for determining mosquito susceptibility to volatile insecticides.

This paper deals with the study of the chemical composition of cow dung and the paper-making from cow dung. With the insight into such data, we considered the effect of cooking temperatures and the potassium hydroxide concentration on paper performance. The relationship between cow dung fiber, such as morphology, chemical composition, crystallization, and chemical state structure, and paper performance were studied. Meanwhile, the mechanical performance of cow dung paper and the degradation of cow dung paper in soil were investigated in this work as well. At 100°C, the residue from cooking cow dung decreased and stabilized. Fiber yield in cow dung peaked at 10% KOH concentration. The lignin-hemicellulose C=O bonds were completely broken making the material softer and looser. At 42 °SR Schopper-Riegler’s Degree (SR) pulping, the polymerization was 1126, and the average fiber length and width were 1245 μm and 18.3 μm. Cow dung paper had the best mechanical performance at 42 °SR of 2.48 KPam2/g burst index, 4.83 mNm2/g tear index, and 26.72 Nm/g tensile index, respectively. This work demonstrates that cellulose fiber, extracted from cow dung, is suitable for paper applications and provides new insight into cow dung recycling.

A mini-review is presented on handmade papers regarding their origin from papyrus to modern paper mill start-ups in the USA. The overview succinctly covers the process of making handmade papers, value addition, and product design. The scientific investigations on obtaining HMP from innovative raw materials such as coffee beans, agro-waste, seaweeds, hyacinth flowers, and others are detailed. The role of handmade paper in conservation along with the environmental and economic benefits of recycled handmade paper are contained within. The mini-review concludes with a promising future for handmade papers that would encourage sustainability.

Access to scientific data can enable independent reuse and verification; however, most data are not available and become increasingly irrecoverable over time. This study aimed to retrieve and preserve important datasets from 160 of the most highly-cited social science articles published between 2008–2013 and 2015–2018. We asked authors if they would share data in a public repository—the Data Ark—or provide reasons if data could not be shared. Of the 160 articles, data for 117 (73%, 95% CI [67%–80%]) were not available and data for 7 (4%, 95% CI [0%–12%]) were available with restrictions. Data for 36 (22%, 95% CI [16%–30%]) articles were available in unrestricted form: 29 of these datasets were already available and 7 datasets were made available in the Data Ark. Most authors did not respond to our data requests and a minority shared reasons for not sharing, such as legal or ethical constraints. These findings highlight an unresolved need to preserve important scientific datasets and increase their accessibility to the scientific community.

The effect of calcium carbonate nanoparticles (CaCO3) on the mechanical and thermal properties of various polymers was investigated in this review. The results were compared to scholarly research published between 2002-2022. Different polymers were evaluated, including Polypropylene (PP), High-density polyethylene (HDPE), Polyvinyl chloride (PVC), Low-density polyethylene (LDPE), Polyethylene (PE), and natural rubber (NR). Through this work, the effect of CaCO3 nanoparticles that act as fillers in polymeric materials has been reviewed. It can be concluded that mechanical and thermal properties can be decreased, increased, or unchanged by increasing and decreasing the fillers to obtain optimal results. It is reasonable to conclude that most papers with nano-CaCO3 showed improvements in appreciable mechanical and thermal properties. In general, the term "surface modification of inorganic fillers" refers to the coating of the fillers with organic materials, which can be done by physical and chemical interactions between the modifiers and the fillers. The reviewed articles revealed that modification of CaCO3 nanoparticles with surface pre-treatment fillers caused enhancement of the mechanical properties of the polymeric matrix twice and prevented the agglomeration of particles in the matrix. Various mixing methods have been used, the most significant being a twin screw extruder, mechanical stirrer, and two-roll mill.

Abstract Osteoclasts are the primary target for osteoporosis drug development. Recent animal studies revealed the crucial roles of osteoblasts in regulating osteoclastogenesis and the longer lifespans of osteoclasts than previously thought with fission and recycling. However, existing culture platforms are limited to replicating these newly identified cellular processes. We report a demineralized bone paper (DBP)-based osteoblast culture and osteoclast assay platform that replicates osteoclast fusion, fission, resorption, and apoptosis with high fidelity and analytical power. An osteoid-inspired DBP supports rapid and structural mineral deposition by osteoblasts. Coculture osteoblasts and bone marrow monocytes under biochemical stimulation recapitulate osteoclast differentiation and function. The DBP-based bone model allows longitudinal quantitative fluorescent monitoring of osteoclast responses to bisphosphonate drug, substantiating significantly reducing their number and lifespan. Finally, we demonstrate the feasibility of humanizing the bone model. The DBP-based osteo assay platforms are expected to advance bone remodeling-targeting drug development with improved prediction of clinical outcomes.

Abstract Uropathogenic Escherichia coli (UPEC) causes up to 90% of urinary tract infections (UTI) which is more prevalent among females than males. In urine, patients with symptomatic UTI usually have a high concentration of bacterial infection, ≥ 105 colony-forming units (CFU) per mL, in which the culture method is regularly the gold standard diagnosis. In this study, a simple and inexpensive distance-based paper device (dPAD) combined with the fluorescent closed tube LAMP assay was validated for simultaneously screening and semi-quantifying the infection level of E. coli in 440 urine samples of patients with UTI. The dPAD could measure the LAMP amplicons and semi-quantify the levels of E. coli infection in heavy (≥ 104 CFU/mL), light (≤ 103 CFU/mL) and no infection. The sensitivity and specificity had reliable performances, achieving as high as 100 and 92.7%, respectively. The one step LAMP assay could be performed within 3 h, which was 7.5 times faster than the culture method. To empower early UTI diagnosis and fast treatment, this inexpensive dPAD tool combined with the fluorescent closed tube LAMP assay is simple, reliably fast and practically portable for point-of-care settings, particularly in resource-limited areas, which can be set up in all levels of healthcare facilities.

Abstract Fluorescence analysis is a fast and sensitive method, and has great potential application in trace detection of environmental toxins. However, many important environmental toxins are non-fluorescent substances, and it is still a challenge to construct a fluorescence detection method for non-fluorescent substances. Here, by means of charge transfer effect and smart molecular imprinting technology, we report a sensitive indirect fluorescent sensing mechanism (IFSM) and microcystin (MC-RR) is selected as a model target. A molecular imprinted thin film is immobilized on the surface of zinc ferrite nanoparticles (ZnFe2O4 NPs) by using arginine, a dummy fragment of MC-RR. By implementation of IFSM on the paper-based microfluidic chip, a versatile platform for the quantitative assay of MC-RR is developed at trace level (the limit of detection of 0.43 μg/L and time of 20 min) in real water samples without any pretreatment. Importantly, the proposed IFSM can be easily modified and extended for the wide variety of species which lack direct interaction with the fluorescent substrate. This work offers the potential possibility to meet the requirements for the on-site analysis and may explore potential applications of molecularly imprinted fluorescent sensors.

Sponsored and organized by the International Open Benchmark Council (BenchCouncil), the IC conference is to provide a pioneering technology map through searching and advancing state-of-the-art and state-of-the-practice in processors, systems, algorithms, and applications for machine learning, deep learning, spiking neural network and other AI techniques across multidisciplinary and interdisciplinary areas. IC 2023 invites manuscripts describing original work in the above areas and topics. All accepted papers will be presented at the IC 2023 conference and published by Springer CCIS (Indexed by EI). The IC conferences have been successfully held for two series from 2019 to 2022 and attracted plenty of paper submissions and participants. IC 2023 will be held on December 4-6, 2023 in Sanya and invites manuscripts describing original work in processors, systems, algorithms, and applications for AI techniques across multidisciplinary and interdisciplinary areas. The conference website is https://www.benchcouncil.org/ic2023/.Important Dates: Paper Submission: July 31, 2023, at 11:59 PM AoE Notification: September 30, 2023, at 11:59 PM AoE Final Papers Due: October 31, 2023, at 11:59 PM AoE Conference Date: December 4-6, 2023 Submission Site: https://ic2023.hotcrp.com/