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In the Southwest of Hubei Province, there are four national nature reserves, namely Houhe, Mulinzi, Qizimeishan and Xingdoushan. Together they form a interconnecting protected area in which rare animals and plants are similar and complementing each other. At the same time, its rich landform types and water resources created an excellent living environment for bryophytes. This paper used the method of combining field investigations and published records to analyze the species richness and composition of bryophytes in these national nature reserves of Southwest Hubei, and to compare the diversity of bryophytes with those in Southeast Chongqing and Northwest Hunan. The results were as follows:(1)There were 601 species of bryophytes of 197 genera and 77 families in the national nature reserve group of Southwest Hubei, accounting for 19.89% and 71.46% of the total number of bryophytes in China and Hubei, respectively. Among them, 27 species are endemic to China, 15 families and 91 genera have only one species each.(2)There are some similarities and complementarities among bryophytes in the four national nature reserves within the national nature reserve group, it conforms to the principle of biodiversity conservation in China.(3)The bryophytes flora compositions in the national nature reserve group in Southwest Hubei are diverse, and the number of bryophytes species is significantly higher than that in Southeast Chongqing and Northwest Hunan. Therefore, the national nature reserve group in Southwest Hubei not only effectively protects large-sized rare and endangered plants and animals, but also nurtures a rich and diverse bryophytes flora, thus an important area for of the conservation bryophytes diversity. [ABSTRACT FROM AUTHOR]