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Objective: To improve the detection sensitivity of COVID-19 and reduce the clinical false negative results, this paper constructed a SARS-CoV-2 RNA capture method which can extract low copy RNA. Methods: The biotin probe in this study was designed based on the conserved region of the SARS-CoV-2 genome sequence. Streptavidin magnetic beads-biotin probe capture method was used to capture SARS-CoV-2 pseudovirus RNA by optimizing capture conditions such as magnetic beads concentration, biotin probes concentration and the number of RNA elution times. SARS-CoV-2 pseudovirus was detected by reverse transcription real-time fluorescence quantitative PCR with reference to the SARS-Co V-2 ORF1ab and N gene primers and TaqMan probes published by WHO. This study used one step and step-by-step real-time fluorescence quantitative PCR detection, single and multiple real-time fluorescence quantitative PCR, and the detection results were compared respectively. Results: The biotin probe can capture the RNA sequence at least1.8K away from the probe binding site in this study. When the biotin probe concentration was 12.5 μM and the concentration of magnetic beads was 333.33 μg/m L, combined with the twice eluent of RNA, the capture efficiency was the highest for low copy SARS-CoV-2pseudovirus RNA. The study results showed that the step-by-step method was more sensitive than one-step method and two TaqMan probes were more sensitive than one TaqMan probe for real-time fluorescence quantitative PCR, and the minimum detection limit of the step-by-step and multiple real-time fluorescence quantitative PCR was 10 copies/μL. The intra-group and repeated experimental groups coefficient of variation was less than 1%. All negative control samples were negative in the study. Conclusions: The streptavidin magnetic beads-biotin probe capture method optimized in this study was an effective method to capture SARS-CoV-2 low copy pseudovirus RNA, the results of RNA extraction were better than some commercial kits, step-by-step and multiple real-time fluorescence quantitative PCR have achieved efficient detection of SARS-CoV-2 low copy pseudovirus RNA, which provides a reference solution for clinical detection of low copy RNA virus. [ABSTRACT FROM AUTHOR]

Biological germplasm resources are strategic resources to ensure national food security, ecological security and human health security, etc. This paper briefly introduces the development of the construction layout, sample preservation, integration and confluence submission of biological germplasm resource banks in China, and the national technology deployment supported by the construction of information platforms. The challenges faced by the biological germplasm resource banks and construction of their information platforms in China were analyzed, and some suggestions for the future development were put forward. [ABSTRACT FROM AUTHOR]

In recent years, with the in-depth development of information technology, its role in people's production and life has become increasingly prominent, and it has played a significant role in improving work efficiency. At the beginning of the new year in 2020, the novel coronavirus pneumonia (COVID-19) outbreak broke out in Wuhan City, Hubei Province and spread rapidly across the country. The country attaches great importance to it, and the medical staff acted quickly and actively participated in this war against the virus without the smoke of gunpowder. After arduous efforts from all over the country, our country's COVID-19 epidemic prevention and control situation has been further consolidated, and the prevention and control work has changed from an emergency state to a normal state of prevention and control. Hospital informatization construction is an objective requirement for hospital modernization and the prevention and control of the COVID-19, and it is of great significance in the context of the normalization of the COVID-19 epidemic prevention and control. This paper elaborates on the four aspects of hospital information construction in the background of COVID-19, the existing problems in hospital information construction, the practice of hospital information construction and the exploration of hospital informatization construction, and is committed to exploring the overall thinking of hospital information construction in special period. [ABSTRACT FROM AUTHOR]

Bladder cancer is a common malignant tumor in the urinary system, the incidence of the disease is increasing year by year, and the recurrence rate is relatively high. Early diagnosis and regular follow-up are the keys to ensure long-term survival of bladder cancer patients. The diagnosis of bladder cancer and follow-up of patients are usually performed by cystoscopy or urine exfoliation cytology. However, the former is more expensive and invasive. The latter has the characteristic of relatively low sensitivity and was limited by the subjective factors of the doctor, there is no urine biomarker that can replace traditional diagnostic methods. Bladder tumors have extensive heterogeneity, and different disease phenotypes have different molecular differences. Therefore, the introduction of urine biomarkers to diagnose the disease and assess the aggressiveness, risk of progression, likelihood of recurrence and prognosis of the disease has important clinical value. In this paper, the current status of urinary biomarkers in the diagnosis of bladder cancer is summarized, and the main research progress in this field is reviewed. [ABSTRACT FROM AUTHOR]

Objective: To investigate clinical efficacy of pediatric massage combined with Xiaoer Xiaoji Zhike oral liquid and azithromycin in the treatment of children of mycoplasma pneumoniae pneumonia (MPP) and its effect on immune function and inflammatory factors. Methods: 80 cases of children with MPP who were treated in our hospital from March 2018 to June 2019 were selected. According to the different treatment methods, they were divided into the control group (azithromycin and Xiaoer Xiaoji Zhike oral liquid treatment, n=40) and the study group (pediatric massage, Xiaoer Xiaoji Zhike oral liquid, azithromycin treatment, n=40). The clinical efficacy of the two groups and the improvement of children's symptoms during the treatment were observed, peripheral blood T lymphocyte subsets, adverse reactions and serum inflammatory factors between the two groups were compared. Results: Compared with the control group, the clinical total effective rate of the study group was higher (P<0.05). Compared with the control group, the study group had a shorter of lung rales, cough and X-ray film of lung lesions to disappear time and antipyretic time (P<0.05). Compared with the control group, CD3+, CD4+ and CD4+/CD8+ in the study group was higher at 14 d after treatment, CD8+ was lower (P<0.05). Compared with the control group, interleukin-1β (IL-1β), C-reactive protein (CRP) and interleukin-6 (IL-6) in the study group were lower (P<0.05). There was no significant difference in incidence of adverse reactions in two groups (P>0.05). Conclusion: Pediatric massage combined with Xiaoer Xiaoji Zhike oral liquid and azithromycin in the treatment of children with MPP can promote the improvement of symptoms, optimize the clinical treatment effect, and effectively regulate the immune function and inflammatory factor levels of children. [ABSTRACT FROM AUTHOR]

Biological aging results in progressive attrition of telomeres, which are the repetitive DNA sequences at the end of chromosomes. The aging-related telomere damage and repair of vascular endothelial cells and smooth muscle cells is considered to be one of the molecular mechanisms of the pathogenesis of degenerative vascular diseases. The aging-related degeneration plays an important role in thoracic aortic aneurysm (TAA), one of the most important fatal diseases in the elderly. This paper reviewed telomeres/telomerase in formation and progression of TAA, and summarizes the regulatory mechanisms of telomere/telomerase in vascular pathology. [ABSTRACT FROM AUTHOR]

Objective: To analyze the clinical characteristics of patients with chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis (PF), and the detection significance of serum transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF) and tumor necrosis factor-α (TNF-α). A total of 80 patients with COPD admitted to the hospital from January 2020 to January 2022 were selected as the study subjects. They were divided into COPD with PF group (n=35) and COPD group (n=45) according to the presence or absence of PF. The clinical manifestations, pulmonary function, blood gas analysis, chest X-ray film, chest CT and HRCT results were analyzed. Serum TGF-β1, CTGF and TNF-α levels were detected. ROC curve was used to analyze the predictive performance of serum TGF-β1, CTGF and TNF-α levels for PF in COPD. The clinical symptoms of patients with COPD and PF were between the two diseases. Pulmonary function test showed mixed ventilation dysfunction, while DIco diffusing capacity showed moderate to severe reduction. Blood gas analysis showed obvious hypoxemia. Chest X-ray film and chest CT showed characteristics of the two diseases. Serum TGF-β1, CTGF and TNF-α levels in the COPD with PF group were significantly higher than those in the COPD group (P<0.05). ROC curve analysis showed that the sensitivity and AUC of serum TGF-β1, CTGF and TNF-α levels in combined diagnosis of COPD complicated with PF were 96.00% and 0.894, higher than those of single diagnosis (P<0.05). Patients with COPD and PF have clinical manifestations of both COPD and PF, which makes the manifestation of each disease atypical, forming unique clinical manifestation, thereby providing a basis for the diagnosis and treatment of the disease. Serum TGF-β1, CTGF and TNF-α levels are high in patients with COPD and PF, suggesting that these indicators can be used to predict COPD complicated with PF. [ABSTRACT FROM AUTHOR]

Objective: To explore and analysis the clinical efficacy of Guizhi Fuzi Decoction combined with Tai Chi in the treatment of cold-damp type sciatica and its influence on the levels of related physical and chemical indicators. Methods: From December 2017 to December 2020, A total of 88 patients with cold-damp sciatica who were diagnosed and treated in our hospital were selected as the research objects. All the patients were divided into the joint group and the matched group with 44 patients in each group accorded to the random 1:1 allocation method. The matched group were given Tai Chi treatment, and the joint group were given Guizhi Fuzi Decoction on the basis of the treatment of the matched group. The clinical curative effect and the changes of related physical and chemical indexes were recorded in the two groups. Results: The total effective rates after treatment in the combined group were 97.7 %, which were higher than 84.1 % in the matched group (P<0.05). The JOA scores of the two groups after treatment were higher than those before treatment, and the joint group were different with that of the matched group (P<0.05). The motor nerve conduction velocity (MCV) and sensory nerve conduction velocity (SCV) of sural nerve were higher in the combined group than in the matched group after treatment (P<0. 05). The serum levels of 5-hydroxytryptamine (5-HT) and Substance P (SP) in the two groups after treatment were different with before treatment, and those in the joint group were lower than those in the matched group (P<0.05). The incidence in the joint group were 6.8 %, which were lower than 31.8 % in the matched group (P<0.05). Conclusion: Guizhi Fuzi Decoction combined with Taijiquan in the treatment of cold-damp type sciatica can inhibit the release of serum 5-HT and SP, improve the nerve conduction velocity of patients, improve the lumbar spine function and overall curative effect, and reduce the occurrence of adverse reactions. [ABSTRACT FROM AUTHOR]